Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia
The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers ampl...
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| Format: | Online |
| Language: | eng |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2019
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/10177 |
| _version_ | 1801706284997672960 |
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| author | Duque-Zapata, Juan Diego Muñoz, Jaime Eduardo Checa-Coral, Oscar |
| author_facet | Duque-Zapata, Juan Diego Muñoz, Jaime Eduardo Checa-Coral, Oscar |
| author_sort | Duque-Zapata, Juan Diego |
| collection | OJS |
| description | The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similarity index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs.The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similarity index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-10177 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | eng |
| publishDate | 2019 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-101772020-08-04T02:23:06Z Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia Caracterización molecular con marcadores SSR para 50 genotipos de arveja arbustiva (Pisum sativum L.) de la Colección GRICAND, Colombia Duque-Zapata, Juan Diego Muñoz, Jaime Eduardo Checa-Coral, Oscar Genetic diversity Genetic structure Pre-breeding SSR markers Grain legumes Plant habit Phytobreeding Diversidad genética Estructura genética Marcadores SSR Pre-mejoramiento Leguminosas de grano Hábito de crecimiento Fitomejoramiento The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similarity index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs.The pea (Pisum sativum L.) is one of the more important legume crops produced globally. We studied the structure and genetic diversity in a collection of 50 pea accessions with 16 simple sequence repeat (SSR) markers, whose average polymorphic information content (PIC) was 0.62. The SSR markers amplified a total of 28 alleles with an average of 4 alleles per locus, with locus AB71 and D21 amplifying the largest number of alleles (6). The observed heterozygosity (Ho) was 0.09±0.08 and the expected heterozygosity (He) was 0.42, indicating an elevated level of inbreeding (Fis = 0.60). The genetic relationships were inferred with a similarity index (DICE) and a bayesian analysis (STRUCTURE), detecting 2 clusters for the genotypes, with a high similarity of the morphological characteristics of each genotype. The results of this study will be useful for the creation of future breeding programs. La arveja (Pisum sativum L.) es uno de los cultivos de leguminosas más importantes producido a nivel mundial. Estudiamos la estructura y diversidad genética en una colección de 50 accesiones de arveja con 16 marcadores de Secuencias simples repetidas (SSR), cuyo promedio del contenido de información polimórfica (PIC) fue de 0,62. Los marcadores SSR amplificaron un total de 28 alelos con un promedio de 4 alelos por locus, siendo el locus AB71 y D21 los que amplificaron el mayor número de alelos (6). La heterocigosidad observada (Ho) fue de 0,09 y la esperada (He) de 0,42, indicando un alto nivel de endogamia (Fis = 0,60). Se infirieron las relaciones genéticas por medio de un análisis de similitud (DICE) y un análisis bayesiano (STRUCTURE) detectando 2 agrupaciones para los genotipos de arveja analizados, con una alta similitud con las características agromorfológicas de cada genotipo. Los resultados del presente estudio serán útiles para la creación de futuros programas de fitomejoramiento en arveja. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2019-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto Texte Testo Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/10177 10.17584/rcch.2019v13i2.10177 Revista Colombiana de Ciencias Hortícolas; Vol. 13 No. 2 (2019); 208-218 Revista Colombiana de Ciencias Hortícolas; Vol. 13 Núm. 2 (2019); 208-218 Revista Colombiana de Ciencias Hortícolas; Vol. 13 No 2 (2019); 208-218 Revista Colombiana de Ciencias Hortícolas; V. 13 N. 2 (2019); 208-218 Revista Colombiana de Ciencias Hortícolas; v. 13 n. 2 (2019); 208-218 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/10177/8678 Colombia; Valle del Cauca; Palmira Colombia; Valle del Cauca; Palmira Colombia; Valle del Cauca; Palmira Colombia; Valle del Cauca; Palmira Colombia; Valle del Cauca; Palmira Copyright (c) 2019 Revista Colombiana de Ciencias Hortícolas |
| spellingShingle | Genetic diversity Genetic structure Pre-breeding SSR markers Grain legumes Plant habit Phytobreeding Diversidad genética Estructura genética Marcadores SSR Pre-mejoramiento Leguminosas de grano Hábito de crecimiento Fitomejoramiento Duque-Zapata, Juan Diego Muñoz, Jaime Eduardo Checa-Coral, Oscar Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia |
| title | Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia |
| title_alt | Caracterización molecular con marcadores SSR para 50 genotipos de arveja arbustiva (Pisum sativum L.) de la Colección GRICAND, Colombia |
| title_full | Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia |
| title_fullStr | Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia |
| title_full_unstemmed | Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia |
| title_short | Molecular characterization using SSR markers in 50 shrub pea genotypes (Pisum sativum L.) from the GRICAND Collection, Colombia |
| title_sort | molecular characterization using ssr markers in 50 shrub pea genotypes pisum sativum l from the gricand collection colombia |
| topic | Genetic diversity Genetic structure Pre-breeding SSR markers Grain legumes Plant habit Phytobreeding Diversidad genética Estructura genética Marcadores SSR Pre-mejoramiento Leguminosas de grano Hábito de crecimiento Fitomejoramiento |
| topic_facet | Genetic diversity Genetic structure Pre-breeding SSR markers Grain legumes Plant habit Phytobreeding Diversidad genética Estructura genética Marcadores SSR Pre-mejoramiento Leguminosas de grano Hábito de crecimiento Fitomejoramiento |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/10177 |
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