Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia
Weeds are a source of new viruses but are often neglected during diversity studies. Eleven weed samples previously were collected along the edges of tomato crops in four municipalities (Florida, Ginebra, Cerrito and Candelaria) in the southeast of Valle del Cauca. These samples were positive for beg...
| Main Authors: | , , |
|---|---|
| Format: | Online |
| Language: | eng |
| Published: |
Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2020
|
| Subjects: | |
| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/10434 |
| _version_ | 1801706285634158592 |
|---|---|
| author | Vaca-Vaca, Juan Carlos Jara-Tejada, Frenyiline López-López, Karina |
| author_facet | Vaca-Vaca, Juan Carlos Jara-Tejada, Frenyiline López-López, Karina |
| author_sort | Vaca-Vaca, Juan Carlos |
| collection | OJS |
| description | Weeds are a source of new viruses but are often neglected during diversity studies. Eleven weed samples previously were collected along the edges of tomato crops in four municipalities (Florida, Ginebra, Cerrito and Candelaria) in the southeast of Valle del Cauca. These samples were positive for begomoviruses, but their molecular characterization had not been done until now. For each sample, DNA fragments were cloned, sequenced and analyzed. Nucleotide sequence analysis of viral fragments showed the presence of six different begomoviruses: two viruses isolated from Lantana camara, Desmodium sp. and Amaranthus dubius, which were previously identified as potato yellow mosaic virus (PYMV) and passionfruit leaf distortion virus (PLDV), respectively; four viruses isolated from L. camara, A. dubius, Rivina humilis, Desmodium sp., Rhynchosia minima, Hybanthus attenuatus, Verbena sp., Croton hirtus and Caesalpinia sp., showed the highest nucleotide sequence identity (89%) with the bean chlorotic mosaic virus (BChMV), datura leaf distortion virus (DaLDV) and rhynchosia golden mosaic Yucatan virus (RhGMYV). The cloned virus fragments isolated from these weeds could be new begomoviruses which have not been previously reported before, according with the ICTV species demarcation criteria established for the Begomovirus genera (≥91 % sequence identity). In this study, mixed infections of begomoviruses were founded in Desmodium sp. and A. dubius. Finally, this paper reports for the first time the identification of three alternative begomovirus weeds: A. dubius for PYMV and L. camara and Desmodium sp. for PLDV, both viruses which infect tomato and passion fruit crops, respectively. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-10434 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | eng |
| publishDate | 2020 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-104342020-12-23T15:26:53Z Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia Caracterización molecular parcial de begomovirus aislados de arvenses colectadas en cultivos de tomate en el sureste del Valle del Cauca, Colombia Vaca-Vaca, Juan Carlos Jara-Tejada, Frenyiline López-López, Karina Geminivirus Hosts Solanaceae Weed Virus Vegetables Geminivirus Hospederos Solanaceae Arvense Weeds are a source of new viruses but are often neglected during diversity studies. Eleven weed samples previously were collected along the edges of tomato crops in four municipalities (Florida, Ginebra, Cerrito and Candelaria) in the southeast of Valle del Cauca. These samples were positive for begomoviruses, but their molecular characterization had not been done until now. For each sample, DNA fragments were cloned, sequenced and analyzed. Nucleotide sequence analysis of viral fragments showed the presence of six different begomoviruses: two viruses isolated from Lantana camara, Desmodium sp. and Amaranthus dubius, which were previously identified as potato yellow mosaic virus (PYMV) and passionfruit leaf distortion virus (PLDV), respectively; four viruses isolated from L. camara, A. dubius, Rivina humilis, Desmodium sp., Rhynchosia minima, Hybanthus attenuatus, Verbena sp., Croton hirtus and Caesalpinia sp., showed the highest nucleotide sequence identity (89%) with the bean chlorotic mosaic virus (BChMV), datura leaf distortion virus (DaLDV) and rhynchosia golden mosaic Yucatan virus (RhGMYV). The cloned virus fragments isolated from these weeds could be new begomoviruses which have not been previously reported before, according with the ICTV species demarcation criteria established for the Begomovirus genera (≥91 % sequence identity). In this study, mixed infections of begomoviruses were founded in Desmodium sp. and A. dubius. Finally, this paper reports for the first time the identification of three alternative begomovirus weeds: A. dubius for PYMV and L. camara and Desmodium sp. for PLDV, both viruses which infect tomato and passion fruit crops, respectively. Las arvenses son una fuente de nuevos virus, pero a menudo se descuidan durante los estudios de diversidad. Previamente, se recolectaron once muestras de arvenses a lo largo de los bordes de un campo de cultivo de tomate ubicado en cuatro municipios (Florida, Ginebra, Cerrito y Candelaria) en el sureste del Valle del Cauca. Estas muestras fueron positivas para begomovirus, pero su caracterización molecular no se había realizado hasta ahora. Para cada muestra, se clonaron, secuenciaron y analizaron fragmentos de DNA. El análisis de la secuencia de nucleótidos de los fragmentos virales mostró la presencia de seis begomovirus diferentes: dos virus aislados de L. camara, Desmodium sp. y A. dubius fueron descritos previamente como el virus del mosaico amarillo de la papa (PYMV) y el virus de distorsión de la hoja de maracuyá (PLDV), respectivamente; otros cuatro virus que se aislaron de L. camara, A. dubius, R. humilis, Desmodium sp., R. minima, H. attenuatus, Verbena sp., C. hirtus y Caesalpinia sp., mostraron su mayor identidad de secuencia de nucleótidos (89%) con el virus del mosaico clorótico del frijol (BChMV), el virus de la distorsión de la hoja de la datura (DaLDV) y el virus del mosaico dorado de rhynchosia de Yucatán (RhGMYV). Los fragmentos de virus clonados de estas malezas podrían ser begomovirus nuevos que no se reportaron anteriormente, esto de acuerdo con el criterio de demarcación de especies de ICTV para el género Begomovirus (≥ 91% de identidad de secuencia). Este análisis también encontró la presencia de infecciones mixtas de begomovirus en las arvenses Desmodium sp. y A. dubius. Finalmente, este artículo reporta por primera vez tres arvenses hospederas alternativas de begomovirus que infectan cultivos de tomate y maracuyá: A. dubius para PYMV, y L. camara y Desmodium sp. para PLDV, respectivamente. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2020-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/10434 10.17584/rcch.2020v14i1.10434 Revista Colombiana de Ciencias Hortícolas; Vol. 14 No. 1 (2020); 115-124 Revista Colombiana de Ciencias Hortícolas; Vol. 14 Núm. 1 (2020); 115-124 Revista Colombiana de Ciencias Hortícolas; Vol. 14 No 1 (2020); 115-124 Revista Colombiana de Ciencias Hortícolas; V. 14 N. 1 (2020); 115-124 Revista Colombiana de Ciencias Hortícolas; v. 14 n. 1 (2020); 115-124 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/10434/9299 Colombia; Valle del Cauca Colombia; Valle del Cauca Copyright (c) 2020 Revista Colombiana de Ciencias Hortícolas |
| spellingShingle | Geminivirus Hosts Solanaceae Weed Virus Vegetables Geminivirus Hospederos Solanaceae Arvense Vaca-Vaca, Juan Carlos Jara-Tejada, Frenyiline López-López, Karina Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia |
| title | Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia |
| title_alt | Caracterización molecular parcial de begomovirus aislados de arvenses colectadas en cultivos de tomate en el sureste del Valle del Cauca, Colombia |
| title_full | Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia |
| title_fullStr | Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia |
| title_full_unstemmed | Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia |
| title_short | Partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of Valle del Cauca, Colombia |
| title_sort | partial molecular characterization of begomoviruses isolated from weeds collected in tomato crops in the southeast of valle del cauca colombia |
| topic | Geminivirus Hosts Solanaceae Weed Virus Vegetables Geminivirus Hospederos Solanaceae Arvense |
| topic_facet | Geminivirus Hosts Solanaceae Weed Virus Vegetables Geminivirus Hospederos Solanaceae Arvense |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/10434 |
| work_keys_str_mv | AT vacavacajuancarlos partialmolecularcharacterizationofbegomovirusesisolatedfromweedscollectedintomatocropsinthesoutheastofvalledelcaucacolombia AT jaratejadafrenyiline partialmolecularcharacterizationofbegomovirusesisolatedfromweedscollectedintomatocropsinthesoutheastofvalledelcaucacolombia AT lopezlopezkarina partialmolecularcharacterizationofbegomovirusesisolatedfromweedscollectedintomatocropsinthesoutheastofvalledelcaucacolombia AT vacavacajuancarlos caracterizacionmolecularparcialdebegomovirusaisladosdearvensescolectadasencultivosdetomateenelsurestedelvalledelcaucacolombia AT jaratejadafrenyiline caracterizacionmolecularparcialdebegomovirusaisladosdearvensescolectadasencultivosdetomateenelsurestedelvalledelcaucacolombia AT lopezlopezkarina caracterizacionmolecularparcialdebegomovirusaisladosdearvensescolectadasencultivosdetomateenelsurestedelvalledelcaucacolombia |
