Didactic intervention for the extraction and quantification of steroids in urine
This article describes a didactic strategy for teaching a miniaturized solid-phase extractionmethod in the context of laboratory practical training [LPT]. It is based on the students’previous imaginaries in order to generate conceptual changes and promote meaningful learning through a few l...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2021
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| Online Access: | https://revistas.uptc.edu.co/index.php/praxis_saber/article/view/11215 |
| _version_ | 1801706445087965184 |
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| author | Angulo, Diego Hernando Casas, Jaime Augusto Cipagauta, Edna Carolina Aparicio, David Eduardo |
| author_facet | Angulo, Diego Hernando Casas, Jaime Augusto Cipagauta, Edna Carolina Aparicio, David Eduardo |
| author_sort | Angulo, Diego Hernando |
| collection | OJS |
| description | This article describes a didactic strategy for teaching a miniaturized solid-phase extractionmethod in the context of laboratory practical training [LPT]. It is based on the students’previous imaginaries in order to generate conceptual changes and promote meaningful learning through a few laboratory sessions. This article will report on the experience of combining the use of a sample preparation technique—PT-SPE—with a didactic intervention exercise for the improvement of procedural skills. We present the results of the application of LPT in the multi-determination of three steroids in urine samples using HPLC-DAD-PT-C18-SPE, which show adequate recovery rates and validation parameters. The results of the intervention show improvement of skills related to solid-phase extraction methods. It is concluded that there were conceptual changes in analytical processes, an adequate familiarization with new sample preparation methodologies, a significant increase in chemical vocabulary,and understanding of concepts, along with the application of lower-order cognitive processes to the enhancement of higher-order cognitive skills. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-11215 |
| institution | Revista Praxis & Saber |
| language | spa |
| publishDate | 2021 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-112152023-02-11T03:46:27Z Didactic intervention for the extraction and quantification of steroids in urine Intervención didáctica para la extracción y cuantificación de esteroides en orina Intervenção didática para a extração e quantificação de esteroides na urina Angulo, Diego Hernando Casas, Jaime Augusto Cipagauta, Edna Carolina Aparicio, David Eduardo experimental science didactics laboratory practical training sample preparation SPE HPLC Didáctica de las ciencias experimentales trabajos prácticos de laboratorio preparación de muestras SPE HPLC didática das ciências experimentais prática laboratorial preparação de amostras SPE HPLC This article describes a didactic strategy for teaching a miniaturized solid-phase extractionmethod in the context of laboratory practical training [LPT]. It is based on the students’previous imaginaries in order to generate conceptual changes and promote meaningful learning through a few laboratory sessions. This article will report on the experience of combining the use of a sample preparation technique—PT-SPE—with a didactic intervention exercise for the improvement of procedural skills. We present the results of the application of LPT in the multi-determination of three steroids in urine samples using HPLC-DAD-PT-C18-SPE, which show adequate recovery rates and validation parameters. The results of the intervention show improvement of skills related to solid-phase extraction methods. It is concluded that there were conceptual changes in analytical processes, an adequate familiarization with new sample preparation methodologies, a significant increase in chemical vocabulary,and understanding of concepts, along with the application of lower-order cognitive processes to the enhancement of higher-order cognitive skills. Este artículo describe una estrategia didáctica para la enseñanza de un método de extracción en fase sólida miniaturizada en contextos del trabajo práctico de laboratorio [TPL]. Se parte de los imaginarios previos de los estudiantes para, a partir de algunas sesiones de laboratorio, generar cambios conceptuales y promover un aprendizaje significativo. En este trabajo se relatará la experiencia de conjugar el empleo de una técnica de preparación de muestras —PT-SPE— con un ejercicio de intervención didáctica para el desarrollo de habilidades procedimentales. Se presentan los resultados de la aplicación de TPL en la multideterminación de tres esteroides en muestras de orina al emplear HPLC-DAD-PT-C18-SPE, que presentan adecuados porcentajes de recuperación y parámetros de validación. Los resultados de la intervención muestran una mejoría en habilidades relacionadas con métodos de extracción en fase sólida. Se concluye que hubo cambios conceptuales frente a procesos analíticos, una adecuada familiarización con nuevas metodologías de preparación de muestras, aumento significativo del lenguaje químico y la comprensión de conceptos, junto con la aplicación de procesos cognitivos de bajo orden, para la promoción de habilidades cognitivas de alto orden. O artigo descreve uma estratégia didática para o ensino de um método de extração em fasesólida miniaturizado no contexto da prática laboratorial [PL]. Isto se baseia nos imagináriosprévios dos estudantes a fim de gerar mudanças conceituais e promover um aprendizadosignificativo através de algumas sessões de laboratório. Este artigo relatará a experiência decombinar o uso de uma técnica de preparação de amostras — PT-SPE — com um exercíciode intervenção didática para o desenvolvimento de habilidades processuais. Apresentam-seos resultados da aplicação de PL na determinação múltipla de três esteroides em amostrasde urina usando HPLC-DAD-PT-C18-SPE, mostrando percentuais de recuperação eparâmetros de validação adequados. Os resultados da intervenção mostram uma melhoriadas habilidades relacionadas aos métodos de extração em fase sólida. Conclui-se que houvemudanças conceituais nos processos analíticos, uma familiarização adequada com as novasmetodologias de preparação de amostras, um aumento significativo no uso do vocabulárioquímico e na compreensão de conceitos, além da aplicação de processos cognitivos de baixaordem para a promoção de habilidades cognitivas de ordem mais elevada. Universidad Pedagógica y Tecnológica de Colombia 2021-03-25 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/xml text/html application/epub+zip https://revistas.uptc.edu.co/index.php/praxis_saber/article/view/11215 10.19053/22160159.v12.n31.2021.11215 Praxis & Saber; Vol. 12 No. 31 (2021): Investigación y pedagogía; e11215 Praxis & Saber; Vol. 12 Núm. 31 (2021): Investigación y pedagogía; e11215 Praxis & Saber; Vol. 12 No 31 (2021): Investigación y pedagogía; e11215 Praxis & Saber; V. 12 N. 31 (2021): Investigación y pedagogía; e11215 Praxis & Saber; v. 12 n. 31 (2021): Investigación y pedagogía; e11215 2462-8603 2216-0159 spa https://revistas.uptc.edu.co/index.php/praxis_saber/article/view/11215/10446 https://revistas.uptc.edu.co/index.php/praxis_saber/article/view/11215/12588 https://revistas.uptc.edu.co/index.php/praxis_saber/article/view/11215/10447 https://revistas.uptc.edu.co/index.php/praxis_saber/article/view/11215/10448 Derechos de autor 2021 Diego Hernando Angulo, Jaime Augusto Casas, Edna Carolina Cipagauta, David Eduardo Aparicio http://creativecommons.org/licenses/by-nc/4.0 |
| spellingShingle | experimental science didactics laboratory practical training sample preparation SPE HPLC Didáctica de las ciencias experimentales trabajos prácticos de laboratorio preparación de muestras SPE HPLC didática das ciências experimentais prática laboratorial preparação de amostras SPE HPLC Angulo, Diego Hernando Casas, Jaime Augusto Cipagauta, Edna Carolina Aparicio, David Eduardo Didactic intervention for the extraction and quantification of steroids in urine |
| title | Didactic intervention for the extraction and quantification of steroids in urine |
| title_alt | Intervención didáctica para la extracción y cuantificación de esteroides en orina Intervenção didática para a extração e quantificação de esteroides na urina |
| title_full | Didactic intervention for the extraction and quantification of steroids in urine |
| title_fullStr | Didactic intervention for the extraction and quantification of steroids in urine |
| title_full_unstemmed | Didactic intervention for the extraction and quantification of steroids in urine |
| title_short | Didactic intervention for the extraction and quantification of steroids in urine |
| title_sort | didactic intervention for the extraction and quantification of steroids in urine |
| topic | experimental science didactics laboratory practical training sample preparation SPE HPLC Didáctica de las ciencias experimentales trabajos prácticos de laboratorio preparación de muestras SPE HPLC didática das ciências experimentais prática laboratorial preparação de amostras SPE HPLC |
| topic_facet | experimental science didactics laboratory practical training sample preparation SPE HPLC Didáctica de las ciencias experimentales trabajos prácticos de laboratorio preparación de muestras SPE HPLC didática das ciências experimentais prática laboratorial preparação de amostras SPE HPLC |
| url | https://revistas.uptc.edu.co/index.php/praxis_saber/article/view/11215 |
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