Gnotobiotic system for selecting microorganisms with biocontrol potential against Fusarium oxysporum f. sp. physali
The cape gooseberry (Physalis peruviana) is a Solanaceae species with enormous economic importance in Colombia; it is the second most exported fruit, after bananas. Vascular wilt caused by Fusarium oxysporum f. sp. physali (Fox) is the most limiting factor of this crop, with losses of up to 80% of p...
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| Format: | Online |
| Language: | eng |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2021
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/11699 |
| _version_ | 1801706291220971520 |
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| author | Garcia, Diana Dávila, Lizeth Lorena González, Adriana Caro, Alejandro Cotes, Alba Marina |
| author_facet | Garcia, Diana Dávila, Lizeth Lorena González, Adriana Caro, Alejandro Cotes, Alba Marina |
| author_sort | Garcia, Diana |
| collection | OJS |
| description | The cape gooseberry (Physalis peruviana) is a Solanaceae species with enormous economic importance in Colombia; it is the second most exported fruit, after bananas. Vascular wilt caused by Fusarium oxysporum f. sp. physali (Fox) is the most limiting factor of this crop, with losses of up to 80% of production. Biological control is a promising alternative for controlling this pathogen. Bacteria and fungi, originally isolated from potentially suppressive soils of cape gooseberry crops in Nariño, Colombia with different management (organic and conventional), were evaluated as biocontrol agents of Fox using a gnotobiotic model (seedlings cultured under axenic conditions with defined microbial strains). Of the 64 isolated microorganisms, 37.5% (15 bacteria and 9 fungi) were discarded because of toxicological risks and an unknow potential biological control. The remaining 62.5% of the microorganisms, 14 bacteria and 26 fungi, were evaluated to assess their potential as biological control agents against Fox. The gnotobiotic model system evaluated the protection and plant growth promotion characteristics. Response variables were used to group the microorganism using a principal component analysis (PCA), and five clusters were obtained. Cluster number four concentrated the 10 microorganisms (three bacteria and seven fungi) with the highest protection values against Fox, with a positive effect on growth. The isolates were identified as two Bacillus subtilis strains, Rhodococcus sp., Podospora setosa, Debaryomyces vindobonensis, Plectosphaerella plurivora, Acinetobacter rhizosphaerae, Umbelopsis sp. and two strains of Trichoderma koningiopsis. The gnobiotic system offered clear advantages for evaluating and selecting microorganisms with a biological control potential against Fusarium oxysporum f. sp. physalis. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-11699 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | eng |
| publishDate | 2021 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-116992021-10-18T21:49:05Z Gnotobiotic system for selecting microorganisms with biocontrol potential against Fusarium oxysporum f. sp. physali Sistema gnotobiótico para seleccionar microorganismos con potencial de biocontrol contra Fusarium oxysporum f. sp. physali Garcia, Diana Dávila, Lizeth Lorena González, Adriana Caro, Alejandro Cotes, Alba Marina Biological control In vitro Vascular wilt Rhizosphere Suppressive soils Golden berry Fusarium oxysporum Golden berry; Fusarium Control biológico In vitro Marchitamiento vascular Rizósfera Suelos supresores Uchuva Fusarium oxysporum Uchuva Fusarium The cape gooseberry (Physalis peruviana) is a Solanaceae species with enormous economic importance in Colombia; it is the second most exported fruit, after bananas. Vascular wilt caused by Fusarium oxysporum f. sp. physali (Fox) is the most limiting factor of this crop, with losses of up to 80% of production. Biological control is a promising alternative for controlling this pathogen. Bacteria and fungi, originally isolated from potentially suppressive soils of cape gooseberry crops in Nariño, Colombia with different management (organic and conventional), were evaluated as biocontrol agents of Fox using a gnotobiotic model (seedlings cultured under axenic conditions with defined microbial strains). Of the 64 isolated microorganisms, 37.5% (15 bacteria and 9 fungi) were discarded because of toxicological risks and an unknow potential biological control. The remaining 62.5% of the microorganisms, 14 bacteria and 26 fungi, were evaluated to assess their potential as biological control agents against Fox. The gnotobiotic model system evaluated the protection and plant growth promotion characteristics. Response variables were used to group the microorganism using a principal component analysis (PCA), and five clusters were obtained. Cluster number four concentrated the 10 microorganisms (three bacteria and seven fungi) with the highest protection values against Fox, with a positive effect on growth. The isolates were identified as two Bacillus subtilis strains, Rhodococcus sp., Podospora setosa, Debaryomyces vindobonensis, Plectosphaerella plurivora, Acinetobacter rhizosphaerae, Umbelopsis sp. and two strains of Trichoderma koningiopsis. The gnobiotic system offered clear advantages for evaluating and selecting microorganisms with a biological control potential against Fusarium oxysporum f. sp. physalis. La uchuva (Physalis peruviana) es Solanaceae de enorme importancia económica en Colombia ya que es la segunda fruta más exportada después del banano. La marchitez vascular causada por Fusarium oxysporum f. sp. physali (Fox) es el factor más limitante de este cultivo, que ocasiona pérdidas de hasta el 80% de su producción. El control biológico se ha convertido en una alternativa prometedora para controlar este patógeno. Bacterias y hongos, originalmente aislados de suelos potencialmente supresores de cultivos de uchuva de Nariño, Colombia con diferente manejo (orgánico y convencional) fueron evaluados como agentes de control de Fox utilizando un modelo gnotobiótico (plántulas cultivadas en condiciones axiológicas con cepas microbianas definidas). A partir de los 64 microorganismos aislados, 37.5% (15 bacterias y nueve hongos) fueron descartados debido a su riesgo toxicológico y a un control biológico potencial desconocido, el 62.5% restante de microorganismos, 14 bacterias y 26 hongos, se evaluaron para evaluar su potencial como agentes de control biológico contra Fox. El sistema de modelos gnotobióticos nos permitió evaluar las características de protección y promoción del crecimiento vegetal, estas variables de respuesta se utilizaron para agrupar el microorganismo utilizando un análisis de componentes principales (PCA) y se obtuvieron cinco clústeres. El clúster número cuatro concentró 10 microorganismos (tres bacterias y siete hongos) con los valores de protección más altos contra Fox y con un efecto positivo en la promoción del crecimiento. Los aislados fueron identificados como dos cepas de Bacillus subtilis, Rhodococcus sp., Podospora setosa, Debaryomyces vindobonensis, Plectosphaerella plurivora, Acinetobacter rhizosphaerae, Umbelopsis sp. y dos cepas de Trichoderma koningiopsis. El sistema gnobiótico presentado ofrece claras ventajas para evaluar y seleccionar microorganismos con un potencial de control biológico contra Fusarium oxysporum f. sp. fisalis. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2021-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/11699 10.17584/rcch.2021v15i1.11699 Revista Colombiana de Ciencias Hortícolas; Vol. 15 No. 1 (2021); e11699 Revista Colombiana de Ciencias Hortícolas; Vol. 15 Núm. 1 (2021); e11699 Revista Colombiana de Ciencias Hortícolas; Vol. 15 No 1 (2021); e11699 Revista Colombiana de Ciencias Hortícolas; V. 15 N. 1 (2021); e11699 Revista Colombiana de Ciencias Hortícolas; v. 15 n. 1 (2021); e11699 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/11699/10110 Colombia; Cundinamarca Colombia; Cundinamarca Copyright (c) 2021 Revista Colombiana de Ciencias Hortícolas http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Biological control In vitro Vascular wilt Rhizosphere Suppressive soils Golden berry Fusarium oxysporum Golden berry; Fusarium Control biológico In vitro Marchitamiento vascular Rizósfera Suelos supresores Uchuva Fusarium oxysporum Uchuva Fusarium Garcia, Diana Dávila, Lizeth Lorena González, Adriana Caro, Alejandro Cotes, Alba Marina Gnotobiotic system for selecting microorganisms with biocontrol potential against Fusarium oxysporum f. sp. physali |
| title | Gnotobiotic system for selecting microorganisms with biocontrol potential against Fusarium oxysporum f. sp. physali |
| title_alt | Sistema gnotobiótico para seleccionar microorganismos con potencial de biocontrol contra Fusarium oxysporum f. sp. physali |
| title_full | Gnotobiotic system for selecting microorganisms with biocontrol potential against Fusarium oxysporum f. sp. physali |
| title_fullStr | Gnotobiotic system for selecting microorganisms with biocontrol potential against Fusarium oxysporum f. sp. physali |
| title_full_unstemmed | Gnotobiotic system for selecting microorganisms with biocontrol potential against Fusarium oxysporum f. sp. physali |
| title_short | Gnotobiotic system for selecting microorganisms with biocontrol potential against Fusarium oxysporum f. sp. physali |
| title_sort | gnotobiotic system for selecting microorganisms with biocontrol potential against fusarium oxysporum f sp physali |
| topic | Biological control In vitro Vascular wilt Rhizosphere Suppressive soils Golden berry Fusarium oxysporum Golden berry; Fusarium Control biológico In vitro Marchitamiento vascular Rizósfera Suelos supresores Uchuva Fusarium oxysporum Uchuva Fusarium |
| topic_facet | Biological control In vitro Vascular wilt Rhizosphere Suppressive soils Golden berry Fusarium oxysporum Golden berry; Fusarium Control biológico In vitro Marchitamiento vascular Rizósfera Suelos supresores Uchuva Fusarium oxysporum Uchuva Fusarium |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/11699 |
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