Extraction of Bacterial DNA from Water Sources of Agricultural Use
Within the agricultural process different types of water bodies are used, therefore knowledge of crop productivity must include the understanding of the flow of microbiological material present in the used water sources. The last methodological approach aims at the identification of bacterial commun...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2021
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/11703 |
| _version_ | 1801706479400517632 |
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| author | Forero Pineda, Nicolas Marín - Suárez, Johana Forero- Ulloa, Fabio Emilio Gómez-Palacio, Andrés |
| author_facet | Forero Pineda, Nicolas Marín - Suárez, Johana Forero- Ulloa, Fabio Emilio Gómez-Palacio, Andrés |
| author_sort | Forero Pineda, Nicolas |
| collection | OJS |
| description | Within the agricultural process different types of water bodies are used, therefore knowledge of crop productivity must include the understanding of the flow of microbiological material present in the used water sources. The last methodological approach aims at the identification of bacterial communities through the analysis of DNA sequences existent in environmental samples. Due to the high contamination that environmental samples may have, it is important to perform an optimal DNA extraction process that allows subsequent molecular analysis by PCR-based methods. For this reason, the objective of this article is to describe a chemical protocol for the extraction of bacterial DNA from water bodies used in local agricultural activities that is simple, efficient, and quick to apply to get good-quality DNA. Bacterial DNA was extracted from water samples obtained from Tota Lake (Boyacá, Colombia) using a modified chemical protocol and compared with a standard commercial method. As a result, bacterial DNA was obtained with a concentration higher than 140 ng/ul and a purity >1.7 A260/280, results which are similar to those obtained with the commercial standard method with a maximum concentration of 45.94 ng/ul and a purity higher than 1.8 A260/280. The results suggest that this DNA extraction protocol is a fast and low-cost method with which high quality and purity DNA is obtained that can be used in any molecular analysis. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-11703 |
| institution | Revista Ciencia y Agricultura |
| language | spa |
| publishDate | 2021 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-117032021-07-13T04:44:21Z Extraction of Bacterial DNA from Water Sources of Agricultural Use Extracción de ADN bacteriano a partir de cuerpos de agua de uso agrícola Forero Pineda, Nicolas Marín - Suárez, Johana Forero- Ulloa, Fabio Emilio Gómez-Palacio, Andrés microbiological contamination bacterial ADN agricultural microbológico contaminación ADN bacteriano agrícola Within the agricultural process different types of water bodies are used, therefore knowledge of crop productivity must include the understanding of the flow of microbiological material present in the used water sources. The last methodological approach aims at the identification of bacterial communities through the analysis of DNA sequences existent in environmental samples. Due to the high contamination that environmental samples may have, it is important to perform an optimal DNA extraction process that allows subsequent molecular analysis by PCR-based methods. For this reason, the objective of this article is to describe a chemical protocol for the extraction of bacterial DNA from water bodies used in local agricultural activities that is simple, efficient, and quick to apply to get good-quality DNA. Bacterial DNA was extracted from water samples obtained from Tota Lake (Boyacá, Colombia) using a modified chemical protocol and compared with a standard commercial method. As a result, bacterial DNA was obtained with a concentration higher than 140 ng/ul and a purity >1.7 A260/280, results which are similar to those obtained with the commercial standard method with a maximum concentration of 45.94 ng/ul and a purity higher than 1.8 A260/280. The results suggest that this DNA extraction protocol is a fast and low-cost method with which high quality and purity DNA is obtained that can be used in any molecular analysis. Dentro del proceso agrícola se usan diferentes tipos de cuerpos de agua, por esto el conocimiento de la productividad del cultivo debe incluir el entendimiento del flujo del material microbiológico presente en las fuentes de agua usadas. La última aproximación metodológica pretende la identificación de comunidades bacterianas mediante el análisis de secuencias de ADN reveladas en muestras ambientales. Debido a la alta contaminación que pueden tener las muestras ambientales es importante llevar a cabo un proceso de extracción de ADN óptimo que permita realizar un posterior análisis molecular por métodos basados en PCR. Por esta razón, el objetivo del presente artículo es describir un protocolo químico de extracción de ADN bacteriano para cuerpos de agua usados en la actividad agrícola local, que sea sencillo, eficiente y rápido de aplicar, para obtener ADN de calidad. A partir de muestras de agua obtenidas del lago de Tota (Boyacá, Colombia), se extrajo ADN bacteriano mediante un protocolo químico modificado y comparado con un método estándar comercial. Como resultado se obtuvo ADN bacteriano con una concentración superior a 140 ng/ul y una pureza >1,7 A260/280, resultados similares a los obtenidos con el método estándar comercial con una concentración máxima de 45,94 ng/ul y una pureza superior a 1,8 A260/280. Los resultados sugieren que este protocolo de extracción de ADN es un método rápido y de bajo costo con el cual se obtiene ADN de elevada calidad y pureza que puede ser utilizado en cualquier análisis molecular. Universidad Pedagógica y Tecnológica de Colombia 2021-02-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion texto application/pdf application/xml https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/11703 10.19053/01228420.v18.n1.2021.11703 Ciencia y Agricultura; Vol. 18 No. 1 (2021): Enero- Abril; 36-45 Ciencia y Agricultura; Vol. 18 Núm. 1 (2021): Enero- Abril; 36-45 2539-0899 spa https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/11703/10460 https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/11703/10648 Copyright (c) 2021 Nicolas Forero Pineda |
| spellingShingle | microbiological contamination bacterial ADN agricultural microbológico contaminación ADN bacteriano agrícola Forero Pineda, Nicolas Marín - Suárez, Johana Forero- Ulloa, Fabio Emilio Gómez-Palacio, Andrés Extraction of Bacterial DNA from Water Sources of Agricultural Use |
| title | Extraction of Bacterial DNA from Water Sources of Agricultural Use |
| title_alt | Extracción de ADN bacteriano a partir de cuerpos de agua de uso agrícola |
| title_full | Extraction of Bacterial DNA from Water Sources of Agricultural Use |
| title_fullStr | Extraction of Bacterial DNA from Water Sources of Agricultural Use |
| title_full_unstemmed | Extraction of Bacterial DNA from Water Sources of Agricultural Use |
| title_short | Extraction of Bacterial DNA from Water Sources of Agricultural Use |
| title_sort | extraction of bacterial dna from water sources of agricultural use |
| topic | microbiological contamination bacterial ADN agricultural microbológico contaminación ADN bacteriano agrícola |
| topic_facet | microbiological contamination bacterial ADN agricultural microbológico contaminación ADN bacteriano agrícola |
| url | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/11703 |
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