Morphology and pathogenicity of Rhizoctonia solani Kühn associated with potato black scurf in Nariño (Colombia)

The objective of the research was to understand the diversity of Rhizoctonia solani in potato crops in Nariño. Tubers with sclerotia were collected from farms in the municipalities of Pasto, Ipiales, Tuquerres and Ospina. In a laboratory, the strains were grouped in categories, selecting 30 for morp...

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Main Authors:	Betancourth-García, Carlos A., Castro-Caicedo, Bertha Lucía, Quiroz-Ojeda, Claudia, Sañudo-Sotelo, Benjamin, Florez-Casanova, Carlos, Salazar-González, Claudia
Format:	Online
Language:	eng
Published:	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2021
Subjects:	Stem canker Soil-borne fungi Sclerotia Solanum tuberosum Potatoes Diseases Cancro de tallo Hongos del suelo Esclerocios Papas Enfermedades
Online Access:	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/11821
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author	Betancourth-García, Carlos A. Castro-Caicedo, Bertha Lucía Quiroz-Ojeda, Claudia Sañudo-Sotelo, Benjamin Florez-Casanova, Carlos Salazar-González, Claudia
author_facet	Betancourth-García, Carlos A. Castro-Caicedo, Bertha Lucía Quiroz-Ojeda, Claudia Sañudo-Sotelo, Benjamin Florez-Casanova, Carlos Salazar-González, Claudia
author_sort	Betancourth-García, Carlos A.
collection	OJS
description	The objective of the research was to understand the diversity of Rhizoctonia solani in potato crops in Nariño. Tubers with sclerotia were collected from farms in the municipalities of Pasto, Ipiales, Tuquerres and Ospina. In a laboratory, the strains were grouped in categories, selecting 30 for morphological and pathogenic studies. In an PDA medium, the daily mycelia growth rate (DMGR), pigmentation, texture, growth pattern (GP) and sclerotia characteristics were determined. The hyphae width and nuclei number were also evaluated. Solanum tuberosum L. Group Phureja seedlings were used in the pathogenicity test. Initially, 494 strains were obtained with diverse cultural characteristics, grouped in 15 categories, selecting two of each one for the research. Of the 30 strains, there were significant differences in the DMGR according to the Tukey test (P=0.05), 96.6% of the strains had an average of 16.6 mm day-1. 15 day-old colonies had cream, beige, brown and salmon colors. 95% of the isolates formed plush mycelium with GP concentric simple rings, complex rings, and scattered and stellate forms. Sclerotia formation began at 6 days (average), and, at 15 days, dispersed arrangement predominated, as well as a peripheral, with brown, beige and cream colors. Three isolates did not produce sclerotia. The hyphae had a mean of 9.7 μm, and the nuclei number ranged between 7.2 - 8.2, without statistical differences. Twenty-four isolates caused 100% plant infection. The results suggest differences between the isolates, associated with levels of pathogenicity or anastomosis groups (AG), characteristics that will be studied in future research.
format	Online
id	oai:oai.revistas.uptc.edu.co:article-11821
institution	Revista Colombiana de Ciencias Hortícolas
language	eng
publishDate	2021
publisher	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
record_format	ojs
spelling	oai:oai.revistas.uptc.edu.co:article-118212021-07-13T03:16:02Z Morphology and pathogenicity of Rhizoctonia solani Kühn associated with potato black scurf in Nariño (Colombia) Morfología y patogenicidad de Rhizoctonia solani Kühn asociado con la costra negra de la papa en Nariño (Colombia) Betancourth-García, Carlos A. Castro-Caicedo, Bertha Lucía Quiroz-Ojeda, Claudia Sañudo-Sotelo, Benjamin Florez-Casanova, Carlos Salazar-González, Claudia Stem canker Soil-borne fungi Sclerotia Solanum tuberosum Potatoes Diseases Cancro de tallo Hongos del suelo Esclerocios Solanum tuberosum Papas Enfermedades The objective of the research was to understand the diversity of Rhizoctonia solani in potato crops in Nariño. Tubers with sclerotia were collected from farms in the municipalities of Pasto, Ipiales, Tuquerres and Ospina. In a laboratory, the strains were grouped in categories, selecting 30 for morphological and pathogenic studies. In an PDA medium, the daily mycelia growth rate (DMGR), pigmentation, texture, growth pattern (GP) and sclerotia characteristics were determined. The hyphae width and nuclei number were also evaluated. Solanum tuberosum L. Group Phureja seedlings were used in the pathogenicity test. Initially, 494 strains were obtained with diverse cultural characteristics, grouped in 15 categories, selecting two of each one for the research. Of the 30 strains, there were significant differences in the DMGR according to the Tukey test (P=0.05), 96.6% of the strains had an average of 16.6 mm day-1. 15 day-old colonies had cream, beige, brown and salmon colors. 95% of the isolates formed plush mycelium with GP concentric simple rings, complex rings, and scattered and stellate forms. Sclerotia formation began at 6 days (average), and, at 15 days, dispersed arrangement predominated, as well as a peripheral, with brown, beige and cream colors. Three isolates did not produce sclerotia. The hyphae had a mean of 9.7 μm, and the nuclei number ranged between 7.2 - 8.2, without statistical differences. Twenty-four isolates caused 100% plant infection. The results suggest differences between the isolates, associated with levels of pathogenicity or anastomosis groups (AG), characteristics that will be studied in future research. El objetivo de la investigación fue conocer la diversidad de Rhizoctonia solani en cultivos de papa en el departamento de Nariño. Se colectaron tubérculos con esclerocios en fincas de los municipios de Pasto, Ipiales, Túquerres y Ospina. En laboratorio, los aislamientos se agruparon en categorías, seleccionando 30 para los estudios morfológicos y patogénicos. En medio PDA, se determinó la tasa diaria de crecimiento micelial (TDCM), color, textura, patrón de crecimiento (PC) y características de los esclerocios. Se evaluó ancho de hifas y número de núcleos. Para pruebas de patogenicidad se utilizaron plántulas de papa Solanum tuberosum L. Group Phureja. Se obtuvieron 494 aislamientos con características culturales variadas, agrupados en 15 categorías, seleccionando dos de cada grupo para la investigación. Los 30 aislamientos mostraron diferencias significativas en la TDCM y según prueba de Tukey (P=0,05%), el 96,6% tuvo un promedio de 16,6 mm día-1. Colonias de 15 días de edad presentaron colores crema, beige, marrón y salmón. El 95% de los aislamientos formaron micelio afelpado con PC radial simple, radial complejo, disperso y estrellado. La formación de esclerocios se inició a los 6 días y a los 15 mostraron disposición dispersa y periférica, con colores marrón, beige y crema. Tres aislamientos no produjeron esclerocios. El ancho de hifas tuvo un promedio de 9,7 μm y entre 7.2-8.2 núcleos, sin diferencias estadísticas. Veinticuatro aislados causaron 100% de infección en las plantas. Los resultados sugieren diferencias entre aislamientos, asociados con patogenicidad o grupos de anastomosis, características que serán estudiadas en futuras investigaciones. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2021-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/11821 10.17584/rcch.2021v15i1.11821 Revista Colombiana de Ciencias Hortícolas; Vol. 15 No. 1 (2021); e11821 Revista Colombiana de Ciencias Hortícolas; Vol. 15 Núm. 1 (2021); e11821 Revista Colombiana de Ciencias Hortícolas; Vol. 15 No 1 (2021); e11821 Revista Colombiana de Ciencias Hortícolas; V. 15 N. 1 (2021); e11821 Revista Colombiana de Ciencias Hortícolas; v. 15 n. 1 (2021); e11821 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/11821/10095 Colombia; Nariño; Pasto, Ipiales, Tuquerres, Ospina Colombia; Nariño; Pasto, Ipiales, Túquerres, Ospina Copyright (c) 2021 Revista Colombiana de Ciencias Hortícolas
spellingShingle	Stem canker Soil-borne fungi Sclerotia Solanum tuberosum Potatoes Diseases Cancro de tallo Hongos del suelo Esclerocios Solanum tuberosum Papas Enfermedades Betancourth-García, Carlos A. Castro-Caicedo, Bertha Lucía Quiroz-Ojeda, Claudia Sañudo-Sotelo, Benjamin Florez-Casanova, Carlos Salazar-González, Claudia Morphology and pathogenicity of Rhizoctonia solani Kühn associated with potato black scurf in Nariño (Colombia)
title	Morphology and pathogenicity of Rhizoctonia solani Kühn associated with potato black scurf in Nariño (Colombia)
title_alt	Morfología y patogenicidad de Rhizoctonia solani Kühn asociado con la costra negra de la papa en Nariño (Colombia)
title_full	Morphology and pathogenicity of Rhizoctonia solani Kühn associated with potato black scurf in Nariño (Colombia)
title_fullStr	Morphology and pathogenicity of Rhizoctonia solani Kühn associated with potato black scurf in Nariño (Colombia)
title_full_unstemmed	Morphology and pathogenicity of Rhizoctonia solani Kühn associated with potato black scurf in Nariño (Colombia)
title_short	Morphology and pathogenicity of Rhizoctonia solani Kühn associated with potato black scurf in Nariño (Colombia)
title_sort	morphology and pathogenicity of rhizoctonia solani kuhn associated with potato black scurf in narino colombia
topic	Stem canker Soil-borne fungi Sclerotia Solanum tuberosum Potatoes Diseases Cancro de tallo Hongos del suelo Esclerocios Solanum tuberosum Papas Enfermedades
topic_facet	Stem canker Soil-borne fungi Sclerotia Solanum tuberosum Potatoes Diseases Cancro de tallo Hongos del suelo Esclerocios Solanum tuberosum Papas Enfermedades
url	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/11821
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