Kinetic Comparison Of Lignin Removal From Sugarcane (Saccharum Officinarum) And Cactus (Opuntia Ficus-Indica) Bagasse By Using Laccase Enzyme
The present study focused to evaluate the effect of a laccase from Aspergillus sp. in order to expose cellulose contained in both sugarcane (Saccharum officinarum, BCA) and Opuntia-ficus cactus (BOF) bagasses. The kinetic parameters of the enzymatic activity were calculated for each of the...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2021
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12449 |
| _version_ | 1801706348685033472 |
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| author | Cardona López, Nubia Rosa Marín, James Alberto Medina Vargas, Óscar Julio Ríos, Luis Alberto Caicedo Pineda, Gerardo Andrés |
| author_facet | Cardona López, Nubia Rosa Marín, James Alberto Medina Vargas, Óscar Julio Ríos, Luis Alberto Caicedo Pineda, Gerardo Andrés |
| author_sort | Cardona López, Nubia Rosa |
| collection | OJS |
| description | The present study focused to evaluate the effect of a laccase from Aspergillus sp. in order to expose cellulose contained in both sugarcane (Saccharum officinarum, BCA) and Opuntia-ficus cactus (BOF) bagasses. The kinetic parameters of the enzymatic activity were calculated for each of the materials, using the initial rates method at various bagasse:solution ratios (10 g:L to 50 g:L) and a total reaction time of 1 h. The assays presented a non-enzymatic removal of extractables by the buffer effect, under the evaluated reaction conditions. On the other hand, there was no dissolution of cellulose or hemicellulose, but there was lignin. Therefore, the results were analyzed, considering the Michaelis-Menten equation and using the disappearance rate of the glignin:L ratio as a basis. Through a Lineweaver-Burk linearization, the best fit of the values was obtained, where Vmax = 14.9566 glignina:L and Km = 40.2011 glignina:L/h for BCA assays (R = 0.9933) and Vmax = 26.8300 glignina:L and Km = 43.5575 glignina:L/h for BOF assays (R = 0.9972). Based on the above, although laccase presented a similar enzymatic affinity for both bagasses, a better catalytic efficiency is predicted towards lignin removal from BOF (0.6160). |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-12449 |
| institution | Revista Ciencia en Desarrollo |
| language | spa |
| publishDate | 2021 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-124492021-12-14T02:35:17Z Kinetic Comparison Of Lignin Removal From Sugarcane (Saccharum Officinarum) And Cactus (Opuntia Ficus-Indica) Bagasse By Using Laccase Enzyme Comparación De La Cinética De Remoción De La Lignina De Bagazos De Caña De Azúcar (Saccharum Officinarum) Y Cactácea (Opuntia Ficus-Indica), Utilizando Enzima Lacasa Cardona López, Nubia Rosa Marín, James Alberto Medina Vargas, Óscar Julio Ríos, Luis Alberto Caicedo Pineda, Gerardo Andrés bagazo de caña, bagazo de cactáceas, lignina, celulosa, lacasa, cinética enzimática The present study focused to evaluate the effect of a laccase from Aspergillus sp. in order to expose cellulose contained in both sugarcane (Saccharum officinarum, BCA) and Opuntia-ficus cactus (BOF) bagasses. The kinetic parameters of the enzymatic activity were calculated for each of the materials, using the initial rates method at various bagasse:solution ratios (10 g:L to 50 g:L) and a total reaction time of 1 h. The assays presented a non-enzymatic removal of extractables by the buffer effect, under the evaluated reaction conditions. On the other hand, there was no dissolution of cellulose or hemicellulose, but there was lignin. Therefore, the results were analyzed, considering the Michaelis-Menten equation and using the disappearance rate of the glignin:L ratio as a basis. Through a Lineweaver-Burk linearization, the best fit of the values was obtained, where Vmax = 14.9566 glignina:L and Km = 40.2011 glignina:L/h for BCA assays (R = 0.9933) and Vmax = 26.8300 glignina:L and Km = 43.5575 glignina:L/h for BOF assays (R = 0.9972). Based on the above, although laccase presented a similar enzymatic affinity for both bagasses, a better catalytic efficiency is predicted towards lignin removal from BOF (0.6160). El objetivo de este estudio fue evaluar el efecto una lacasa proveniente de Aspergillus sp., para exponer la celulosa en los bagazos de caña de azúcar (Saccharum officinarum) (BCA) y cactácea (Opuntia-ficus) (BOF). Para ello, se llevó a cabo la determinación de los parámetros cinéticos de las enzimas en cada uno de los materiales, en términos de velocidades iniciales, a diversas proporciones bagazo:solución (10 g:L a 50 g:L), con un tiempo total de reacción de 1 h. Bajo las condiciones de reacción evaluadas, se evidenció remoción de extraíbles; sin embargo, esto no fue debido a la acción enzimática, sino por efecto del búfer. Por otra parte, no hubo disolución de celulosa ni hemicelulosa, pero sí de lignina. Por lo tanto, los resultados se analizaron teniendo en cuenta la ecuación de Michaelis-Menten, utilizando como base la tasa de desaparición de la proporción glignina:L. Por medio de una linealización de Lineweaver-Burk, se obtuvo el mejor ajuste de valores, donde Vmax = 14.9566 glignina:L y Km = 40.2011 glignina:L/h para el BCA (R = 0.9933) y Vmax = 26.8300 glignina:L y Km = 43.5575 glignina:L/h para el BOF (R = 0.9972). Con base en lo anterior, aunque la lacasa presentó una afinidad enzimática similar para ambos bagazos, se predice una mejor eficiencia catalítica hacia la remoción de lignina de BOF (0.6160). Universidad Pedagógica y Tecnológica de Colombia 2021-01-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Type="text" application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12449 10.19053/01217488.v12.n1.2021.12449 Ciencia En Desarrollo; Vol. 12 No. 1 (2021): Vol 12, Núm.1 (2021): Enero-Junio; 79-89 Ciencia en Desarrollo; Vol. 12 Núm. 1 (2021): Vol 12, Núm.1 (2021): Enero-Junio; 79-89 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12449/10695 Derechos de autor 2021 CIENCIA EN DESARROLLO |
| spellingShingle | bagazo de caña, bagazo de cactáceas, lignina, celulosa, lacasa, cinética enzimática Cardona López, Nubia Rosa Marín, James Alberto Medina Vargas, Óscar Julio Ríos, Luis Alberto Caicedo Pineda, Gerardo Andrés Kinetic Comparison Of Lignin Removal From Sugarcane (Saccharum Officinarum) And Cactus (Opuntia Ficus-Indica) Bagasse By Using Laccase Enzyme |
| title | Kinetic Comparison Of Lignin Removal From Sugarcane (Saccharum Officinarum) And Cactus (Opuntia Ficus-Indica) Bagasse By Using Laccase Enzyme |
| title_alt | Comparación De La Cinética De Remoción De La Lignina De Bagazos De Caña De Azúcar (Saccharum Officinarum) Y Cactácea (Opuntia Ficus-Indica), Utilizando Enzima Lacasa |
| title_full | Kinetic Comparison Of Lignin Removal From Sugarcane (Saccharum Officinarum) And Cactus (Opuntia Ficus-Indica) Bagasse By Using Laccase Enzyme |
| title_fullStr | Kinetic Comparison Of Lignin Removal From Sugarcane (Saccharum Officinarum) And Cactus (Opuntia Ficus-Indica) Bagasse By Using Laccase Enzyme |
| title_full_unstemmed | Kinetic Comparison Of Lignin Removal From Sugarcane (Saccharum Officinarum) And Cactus (Opuntia Ficus-Indica) Bagasse By Using Laccase Enzyme |
| title_short | Kinetic Comparison Of Lignin Removal From Sugarcane (Saccharum Officinarum) And Cactus (Opuntia Ficus-Indica) Bagasse By Using Laccase Enzyme |
| title_sort | kinetic comparison of lignin removal from sugarcane saccharum officinarum and cactus opuntia ficus indica bagasse by using laccase enzyme |
| topic | bagazo de caña, bagazo de cactáceas, lignina, celulosa, lacasa, cinética enzimática |
| topic_facet | bagazo de caña, bagazo de cactáceas, lignina, celulosa, lacasa, cinética enzimática |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12449 |
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