Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates
Potyviruses are the largest genus of plant viruses that cause significant losses over a wide range of crops. In this paper, the presence of potyvirus in different plant crops in the provinces of Ocaña and Pamplona located in the north and south of the Department of Norte de Santander (Colombia) was...
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2021
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/12481 |
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| author | Chaves-Bedoya, Giovanni Ortíz-Rojas, Luz Yineth |
| author_facet | Chaves-Bedoya, Giovanni Ortíz-Rojas, Luz Yineth |
| author_sort | Chaves-Bedoya, Giovanni |
| collection | OJS |
| description | Potyviruses are the largest genus of plant viruses that cause significant losses over a wide range of crops. In this paper, the presence of potyvirus in different plant crops in the provinces of Ocaña and Pamplona located in the north and south of the Department of Norte de Santander (Colombia) was evaluated with RT-PCR analysis using universal oligonucleotides specific to the region that encodes the NIB protein. The results indicate the presence of several potyvirus in Pamplona in economically important crops such as corn (Zea mays), tomato (Solanum lycopersicum), potato (Solanum tuberosum) and zucchini (Cucurbita pepo). In Ocaña, potyvirus was found in bean (Phaseolus vulgaris), corn and pumpkin (Cucurbita maxima/i>). In corn, one of the most important crops, the presence of the Sugarcane mosaic virus (SCMV) was confirmed with nucleotide sequencing. This is the first report of this virus in the department. The presence of several potyviruses in different crops in Norte de Santander indicate an alarming phytosanitary condition that must be addressed with priority to establish detection and control systems that maximize production, ensure agricultural sustainability, and propose certification schemes and improvement programs to reduce economic losses. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-12481 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | eng |
| publishDate | 2021 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
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| spelling | oai:oai.revistas.uptc.edu.co:article-124812021-11-03T13:59:28Z Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates Presencia de Potyvirus en el departamento de Norte de Santander y caracterización molecular de la proteína NIb en aislamientos colombianos de SCMV Chaves-Bedoya, Giovanni Ortíz-Rojas, Luz Yineth Potyvirus Molecular diagnosis Phytopathology Plant virus Virus Corn Sugarcane Potyvirus Diagnóstico molecular Fitopatología Virus vegetal Virus Maíz Caña de azucar Potyviruses are the largest genus of plant viruses that cause significant losses over a wide range of crops. In this paper, the presence of potyvirus in different plant crops in the provinces of Ocaña and Pamplona located in the north and south of the Department of Norte de Santander (Colombia) was evaluated with RT-PCR analysis using universal oligonucleotides specific to the region that encodes the NIB protein. The results indicate the presence of several potyvirus in Pamplona in economically important crops such as corn (Zea mays), tomato (Solanum lycopersicum), potato (Solanum tuberosum) and zucchini (Cucurbita pepo). In Ocaña, potyvirus was found in bean (Phaseolus vulgaris), corn and pumpkin (Cucurbita maxima/i>). In corn, one of the most important crops, the presence of the Sugarcane mosaic virus (SCMV) was confirmed with nucleotide sequencing. This is the first report of this virus in the department. The presence of several potyviruses in different crops in Norte de Santander indicate an alarming phytosanitary condition that must be addressed with priority to establish detection and control systems that maximize production, ensure agricultural sustainability, and propose certification schemes and improvement programs to reduce economic losses. Los potyvirus son el género más grande de virus de plantas que ocasionan pérdidas significativas en un amplio rango de cultivos. En este trabajo mediante análisis de RT-PCR con oligonucleótidos universales específicos para la región que codifica la proteína NIb, se evaluó la presencia de potyvirus en diferentes plantas de cultivo en las provincias de Ocaña y Pamplona ubicados al norte y sur del departamento de Norte de Santander respectivamente. Los resultados indican la presencia de potyvirus en la provincia de Pamplona en plantas de importancia económica como maíz (Zea mays), tomate (Solanum lycopersicum), papa (Solanum tuberosum) y calabacín (Cucurbita pepo); y en la provincia de Ocaña en plantas de frijol (Phaseolus vulgaris), maíz y ahuyama (Cucurbita maxima). En maíz se confirmó mediante secuenciación nucleotídica la presencia del virus del mosaico de la caña de azúcar (SCMV), por lo que este se constituye el primer reporte de este virus en el departamento. Los resultados positivos por RT-PCR de la presencia de potyvirus en diferentes cultivos en Norte de Santander indican la necesidad apremiante de establecer sistemas de detección y control que permitan maximizar la producción, asegurar la sustentabilidad agrícola, proponer esquemas de certificación y programas de mejoramiento para reducir las pérdidas económicas. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2021-05-18 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/12481 10.17584/rcch.2021v15i2.12481 Revista Colombiana de Ciencias Hortícolas; Vol. 15 No. 2 (2021); e12481 Revista Colombiana de Ciencias Hortícolas; Vol. 15 Núm. 2 (2021); e12481 Revista Colombiana de Ciencias Hortícolas; Vol. 15 No 2 (2021); e12481 Revista Colombiana de Ciencias Hortícolas; V. 15 N. 2 (2021); e12481 Revista Colombiana de Ciencias Hortícolas; v. 15 n. 2 (2021); e12481 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/12481/10588 Colombia; Norte de Santander Colombia; Norte de Santander Copyright (c) 2021 Revista Colombiana de Ciencias Hortícolas |
| spellingShingle | Potyvirus Molecular diagnosis Phytopathology Plant virus Virus Corn Sugarcane Potyvirus Diagnóstico molecular Fitopatología Virus vegetal Virus Maíz Caña de azucar Chaves-Bedoya, Giovanni Ortíz-Rojas, Luz Yineth Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates |
| title | Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates |
| title_alt | Presencia de Potyvirus en el departamento de Norte de Santander y caracterización molecular de la proteína NIb en aislamientos colombianos de SCMV |
| title_full | Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates |
| title_fullStr | Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates |
| title_full_unstemmed | Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates |
| title_short | Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates |
| title_sort | presence of potyvirus in the norte de santander department and molecular characterization of nib protein in colombian scmv isolates |
| topic | Potyvirus Molecular diagnosis Phytopathology Plant virus Virus Corn Sugarcane Potyvirus Diagnóstico molecular Fitopatología Virus vegetal Virus Maíz Caña de azucar |
| topic_facet | Potyvirus Molecular diagnosis Phytopathology Plant virus Virus Corn Sugarcane Potyvirus Diagnóstico molecular Fitopatología Virus vegetal Virus Maíz Caña de azucar |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/12481 |
| work_keys_str_mv | AT chavesbedoyagiovanni presenceofpotyvirusinthenortedesantanderdepartmentandmolecularcharacterizationofnibproteinincolombianscmvisolates AT ortizrojasluzyineth presenceofpotyvirusinthenortedesantanderdepartmentandmolecularcharacterizationofnibproteinincolombianscmvisolates AT chavesbedoyagiovanni presenciadepotyviruseneldepartamentodenortedesantanderycaracterizacionmoleculardelaproteinanibenaislamientoscolombianosdescmv AT ortizrojasluzyineth presenciadepotyviruseneldepartamentodenortedesantanderycaracterizacionmoleculardelaproteinanibenaislamientoscolombianosdescmv |
