Glass sheets coated with TiO2-m and its cytotoxic effect on leukemic cells Molt4
Photodynamic therapy (PDT) has become an important alternative for the treatment of different types of cancer, due to its great advantages over conventional methods. In this field, TiO2 nanoparticles are one of the most widely studied photosensitizers due to their high photocatalytic activity, high...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2021
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12819 |
| _version_ | 1801706350232731648 |
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| author | Garcia-Diosa, Jaime A. Camargo-Amado, Rubén J. |
| author_facet | Garcia-Diosa, Jaime A. Camargo-Amado, Rubén J. |
| author_sort | Garcia-Diosa, Jaime A. |
| collection | OJS |
| description | Photodynamic therapy (PDT) has become an important alternative for the treatment of different types of cancer, due to its great advantages over conventional methods. In this field, TiO2 nanoparticles are one of the most widely studied photosensitizers due to their high photocatalytic activity, high absorption of UVA light and high biocompatibility. Although there are several successful studies related to PDT treatment, using TiO2nanoparticles, for different types of adherent cancer cells, there are few reports of PDT applied to non-adherent cancer cells, due to the challenges related to blood volume and light penetration. To address this challenge, modified TiO2 nanoparticles (TiO2-m) were deposited on glass substrates to study the cytotoxic effect of PDT on Molt4, non-adherent cells, using these coatings. The shaping of uniformed coatings on substrates was proved by standard deviation of transmittance in the UVA range at different points. Different experimental PDT tests of Molt4 cells on TiO2-m coatings were performed and the treatment cytotoxic effect was evaluated by LDH concentration. The results demonstrated the possibility of generating cytotoxic effect on non-adherent cancer cells with only 20 min of contact and 40 min of UVA radiation. This result is the first step for the possible development of new methodologies in the application of PDT on non-adherent cells using TiO2-m coatings |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-12819 |
| institution | Revista Ciencia en Desarrollo |
| language | spa |
| publishDate | 2021 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-128192021-12-14T02:35:17Z Glass sheets coated with TiO2-m and its cytotoxic effect on leukemic cells Molt4 Láminas de vidrio recubiertas con TiO2-m y su efecto citotóxico sobre células leucémicas Molt4 Garcia-Diosa, Jaime A. Camargo-Amado, Rubén J. Terapia Fotodinámica, dióxido de titanio, leucemia, cáncer, recubrimiento nanoestructurado Photodynamic therapy, titanium dioxide, leukemia, cancer, nanostructured coating Photodynamic therapy (PDT) has become an important alternative for the treatment of different types of cancer, due to its great advantages over conventional methods. In this field, TiO2 nanoparticles are one of the most widely studied photosensitizers due to their high photocatalytic activity, high absorption of UVA light and high biocompatibility. Although there are several successful studies related to PDT treatment, using TiO2nanoparticles, for different types of adherent cancer cells, there are few reports of PDT applied to non-adherent cancer cells, due to the challenges related to blood volume and light penetration. To address this challenge, modified TiO2 nanoparticles (TiO2-m) were deposited on glass substrates to study the cytotoxic effect of PDT on Molt4, non-adherent cells, using these coatings. The shaping of uniformed coatings on substrates was proved by standard deviation of transmittance in the UVA range at different points. Different experimental PDT tests of Molt4 cells on TiO2-m coatings were performed and the treatment cytotoxic effect was evaluated by LDH concentration. The results demonstrated the possibility of generating cytotoxic effect on non-adherent cancer cells with only 20 min of contact and 40 min of UVA radiation. This result is the first step for the possible development of new methodologies in the application of PDT on non-adherent cells using TiO2-m coatings La terapia fotodinámica (PDT) se ha convertido en una alternativa importante para el tratamiento de diferentes tipos de cáncer, debido a sus grandes ventajas frente a métodos convencionales. En este campo, las nanopartículas de TiO2 son uno de los fotosensibilizadores más ampliamente estudiados, por su alta actividad fotocatalítica, alta absorción de radiación UVA y alta biocompatibilidad. Aunque hay varios estudios satisfactorios relacionados con el tratamiento PDT, usando nanopartículas de TiO2, para diferentes tipos de células cancerosas adherentes, existen muy pocos reportes de esta metodología aplicada para células no adherentes, debido a los retos relacionados con el volumen de tratamiento y el alcance de la radiación. Para afrontar este reto, nanopartículas de TiO2 modificadas (TiO2-m) se depositaron sobre substratos de vidrio para estudiar el efecto citotóxico de PDT sobre células Molt4, células no adherentes, usando estos recubrimientos. Se comprobó la formación de recubrimientos uniformes sobre los substratos mediante la desviación estándar de la transmitancia en el rango UVA, en diferentes puntos. Se realizaron diferentes pruebas experimentales de PDT de células Molt4 sobre recubrimientos de TiO2-m y el efecto citotóxico de los tratamientos fue evaluado por medio de concentración LDH. Los resultados demostraron la posibilidad de generar un efecto citotóxico de células cancerosas no adherentes con solo 20 min de contacto y 40 min de radiación UVA. Este resultado es el primer avance para el posible desarrollo de nuevas metodologías en la aplicación de PDT en células no adherentes usando recubrimientos de TiO2-m. Universidad Pedagógica y Tecnológica de Colombia 2021-01-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12819 10.19053/01217488.v12.n1.2021.12819 Ciencia En Desarrollo; Vol. 12 No. 1 (2021): Vol 12, Núm.1 (2021): Enero-Junio; 91-97 Ciencia en Desarrollo; Vol. 12 Núm. 1 (2021): Vol 12, Núm.1 (2021): Enero-Junio; 91-97 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12819/10687 Derechos de autor 2021 CIENCIA EN DESARROLLO |
| spellingShingle | Terapia Fotodinámica, dióxido de titanio, leucemia, cáncer, recubrimiento nanoestructurado Photodynamic therapy, titanium dioxide, leukemia, cancer, nanostructured coating Garcia-Diosa, Jaime A. Camargo-Amado, Rubén J. Glass sheets coated with TiO2-m and its cytotoxic effect on leukemic cells Molt4 |
| title | Glass sheets coated with TiO2-m and its cytotoxic effect on leukemic cells Molt4 |
| title_alt | Láminas de vidrio recubiertas con TiO2-m y su efecto citotóxico sobre células leucémicas Molt4 |
| title_full | Glass sheets coated with TiO2-m and its cytotoxic effect on leukemic cells Molt4 |
| title_fullStr | Glass sheets coated with TiO2-m and its cytotoxic effect on leukemic cells Molt4 |
| title_full_unstemmed | Glass sheets coated with TiO2-m and its cytotoxic effect on leukemic cells Molt4 |
| title_short | Glass sheets coated with TiO2-m and its cytotoxic effect on leukemic cells Molt4 |
| title_sort | glass sheets coated with tio2 m and its cytotoxic effect on leukemic cells molt4 |
| topic | Terapia Fotodinámica, dióxido de titanio, leucemia, cáncer, recubrimiento nanoestructurado Photodynamic therapy, titanium dioxide, leukemia, cancer, nanostructured coating |
| topic_facet | Terapia Fotodinámica, dióxido de titanio, leucemia, cáncer, recubrimiento nanoestructurado Photodynamic therapy, titanium dioxide, leukemia, cancer, nanostructured coating |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12819 |
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