Hydrolysis Optimization For The Lignocellulostic Waste Treatment With Aspergillus Niger Enzymes

The present study aimed to optimize the enzymatic hydrolysis process to degrade cellulose from corn stubble (Zea mays), using enzymes from Aspergillus niger. During the first phase of the experimentation, the content of dry matter, organic matter, ash, crude protein, ethereal extract, lignin and cel...

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Main Authors:	Olivo Urbina, Mylena Alexandra, Díaz Monroy, Byron Leoncio, Haro Barroso, Camilo, Borras Sandoval, Luis Miguel
Format:	Online
Language:	spa
Published:	Universidad Pedagógica y Tecnológica de Colombia 2021
Subjects:	Aspergillus niger, azúcares reductores, biotecnología, hidrólisis enzimática, residuos lignocelulósicos Aspergillus niger, reducing sugars, biotechnology, enzymatic hydrolysis, lignocellulosic residues
Online Access:	https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12820
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author	Olivo Urbina, Mylena Alexandra Díaz Monroy, Byron Leoncio Haro Barroso, Camilo Borras Sandoval, Luis Miguel
author_facet	Olivo Urbina, Mylena Alexandra Díaz Monroy, Byron Leoncio Haro Barroso, Camilo Borras Sandoval, Luis Miguel
author_sort	Olivo Urbina, Mylena Alexandra
collection	OJS
description	The present study aimed to optimize the enzymatic hydrolysis process to degrade cellulose from corn stubble (Zea mays), using enzymes from Aspergillus niger. During the first phase of the experimentation, the content of dry matter, organic matter, ash, crude protein, ethereal extract, lignin and cellulose content of the stubble was determined, using the reference methods of the U. FLORIDA 1970 for its quantification. Subsequently the pre-drying and grinding of the same was carried out, prior to the alkali and acid-alkali pre-treatment processes applied, being dried at 4 different temperatures (45, 50, 55 and 60 ° C). The pretreated residues were subjected to hydrolysis with Aspergillus Niger cellulases (39,125 EU) at different pHs (4.0, 4.8 and 5.6), for 24, 48 and 72 h. at 45, 50, 55 and 60 ° C temperature; quantifying the concentrations of reducing sugars released during digestion. Finally, it was determined that the hydrolysis of the stubble without pretreatment and drying at 60 ° C, at a pH of 4.8 for 72 h., had high concentrations of reducing sugars, becoming an alternative for its use as food or biofuel, after the application of biotechnological processes.
format	Online
id	oai:oai.revistas.uptc.edu.co:article-12820
institution	Revista Ciencia en Desarrollo
language	spa
publishDate	2021
publisher	Universidad Pedagógica y Tecnológica de Colombia
record_format	ojs
spelling	oai:oai.revistas.uptc.edu.co:article-128202021-12-14T02:35:17Z Hydrolysis Optimization For The Lignocellulostic Waste Treatment With Aspergillus Niger Enzymes Optimización De La Hidrólisis Para El Tratamiento De Residuos Lignocelulósicos Con Enzimas De Aspergillus Niger Olivo Urbina, Mylena Alexandra Díaz Monroy, Byron Leoncio Haro Barroso, Camilo Borras Sandoval, Luis Miguel Aspergillus niger, azúcares reductores, biotecnología, hidrólisis enzimática, residuos lignocelulósicos Aspergillus niger, reducing sugars, biotechnology, enzymatic hydrolysis, lignocellulosic residues The present study aimed to optimize the enzymatic hydrolysis process to degrade cellulose from corn stubble (Zea mays), using enzymes from Aspergillus niger. During the first phase of the experimentation, the content of dry matter, organic matter, ash, crude protein, ethereal extract, lignin and cellulose content of the stubble was determined, using the reference methods of the U. FLORIDA 1970 for its quantification. Subsequently the pre-drying and grinding of the same was carried out, prior to the alkali and acid-alkali pre-treatment processes applied, being dried at 4 different temperatures (45, 50, 55 and 60 ° C). The pretreated residues were subjected to hydrolysis with Aspergillus Niger cellulases (39,125 EU) at different pHs (4.0, 4.8 and 5.6), for 24, 48 and 72 h. at 45, 50, 55 and 60 ° C temperature; quantifying the concentrations of reducing sugars released during digestion. Finally, it was determined that the hydrolysis of the stubble without pretreatment and drying at 60 ° C, at a pH of 4.8 for 72 h., had high concentrations of reducing sugars, becoming an alternative for its use as food or biofuel, after the application of biotechnological processes. El presente estudio tuvo por objeto optimizar el proceso de hidrólisis enzimática para degradar la celulosa del rastrojo de maíz (Zea mays), utilizando enzimas del Aspergillus niger. Durante la primera fase de la experimentación se determinó el contenido de materia seca, materia orgánica, cenizas, proteína cruda, extracto etéreo, contenido de lignina y celulosa del rastrojo, usándose para su cuantificación los métodos de referencia de la U. FLORIDA 1970. Posteriormente se realizó el pre-secado y molienda del mismo, previo a los procesos de pre-tratamiento álcali y ácido – álcali aplicados, siendo secados a 4 diferentes temperaturas (45, 50, 55 y 60 °C). Los residuos pretratados fueron sometidos a una hidrólisis con celulasas del Aspergillus Niger (39,125 UE) a diferentes pH (4,0; 4,8 y 5,6), durante 24, 48 y 72 h. a 45, 50, 55 Y 60 °C de temperatura; cuantificándose las concentraciones de azúcares reductores liberados durante la digestión. Finalmente se determinó que la hidrólisis del rastrojo sin pretratamiento y secado a 60°C, a pH de 4,8 por 72 h.,  tuvo concentraciones elevadas de azúcares reductores, convirtiéndose en una alternativa para su utilización como alimento o biocombustible, posterior a la aplicación de procesos biotecnológicos. Universidad Pedagógica y Tecnológica de Colombia 2021-01-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12820 10.19053/01217488.v12.n1.2021.12820 Ciencia En Desarrollo; Vol. 12 No. 1 (2021): Vol 12, Núm.1 (2021): Enero-Junio; 99-111 Ciencia en Desarrollo; Vol. 12 Núm. 1 (2021): Vol 12, Núm.1 (2021): Enero-Junio; 99-111 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12820/10686 Derechos de autor 2021 CIENCIA EN DESARROLLO
spellingShingle	Aspergillus niger, azúcares reductores, biotecnología, hidrólisis enzimática, residuos lignocelulósicos Aspergillus niger, reducing sugars, biotechnology, enzymatic hydrolysis, lignocellulosic residues Olivo Urbina, Mylena Alexandra Díaz Monroy, Byron Leoncio Haro Barroso, Camilo Borras Sandoval, Luis Miguel Hydrolysis Optimization For The Lignocellulostic Waste Treatment With Aspergillus Niger Enzymes
title	Hydrolysis Optimization For The Lignocellulostic Waste Treatment With Aspergillus Niger Enzymes
title_alt	Optimización De La Hidrólisis Para El Tratamiento De Residuos Lignocelulósicos Con Enzimas De Aspergillus Niger
title_full	Hydrolysis Optimization For The Lignocellulostic Waste Treatment With Aspergillus Niger Enzymes
title_fullStr	Hydrolysis Optimization For The Lignocellulostic Waste Treatment With Aspergillus Niger Enzymes
title_full_unstemmed	Hydrolysis Optimization For The Lignocellulostic Waste Treatment With Aspergillus Niger Enzymes
title_short	Hydrolysis Optimization For The Lignocellulostic Waste Treatment With Aspergillus Niger Enzymes
title_sort	hydrolysis optimization for the lignocellulostic waste treatment with aspergillus niger enzymes
topic	Aspergillus niger, azúcares reductores, biotecnología, hidrólisis enzimática, residuos lignocelulósicos Aspergillus niger, reducing sugars, biotechnology, enzymatic hydrolysis, lignocellulosic residues
topic_facet	Aspergillus niger, azúcares reductores, biotecnología, hidrólisis enzimática, residuos lignocelulósicos Aspergillus niger, reducing sugars, biotechnology, enzymatic hydrolysis, lignocellulosic residues
url	https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/12820
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