SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae)
This study was the first evaluation of the genetic diversity of cultivated populations of habanero pepper (Capsicum chinense) in Colombia using SSR-HRM. Three habanero pepper lines were characterized with eight microsatellite markers using the High-Resolution Melt (HRM) technique. Twenty-seven indiv...
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| Format: | Online |
| Language: | eng |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2022
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13363 |
| _version_ | 1801706302200610816 |
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| author | Lopéz-Candelo, José Enrique Viáfara-Vega, Ronald Andrés Cárdenas-Henao, Heiber |
| author_facet | Lopéz-Candelo, José Enrique Viáfara-Vega, Ronald Andrés Cárdenas-Henao, Heiber |
| author_sort | Lopéz-Candelo, José Enrique |
| collection | OJS |
| description | This study was the first evaluation of the genetic diversity of cultivated populations of habanero pepper (Capsicum chinense) in Colombia using SSR-HRM. Three habanero pepper lines were characterized with eight microsatellite markers using the High-Resolution Melt (HRM) technique. Twenty-seven individuals from the HL-original line and 30 individuals each from derived lines HL-70 and HL67 were genotyped. Three microsatellites were monomorphic, and five were polymorphic; however, a high allelic diversity was detected in the homozygous state in the 87 individuals. The Ng8 marker differentiated the HL-original and HL-67 lines from the HL-70 line with HRM profiles. The analysis of molecular variance (AMOVA) revelated that 52% of the genetic variation existed within lines. The HL-67 line was more similar to the HL-original line than to the HL-70 line. HL-70 recorded the highest genetic diversity for the derived lines and, therefore, could be used in a new breeding program. In contrast, the HL-67 line, because of its high genetic homogeneity, could potentially be used to evaluate different environmental conditions to find optimal conditions that increase productivity and pungency. Finally, comparing the HRM profiles with the monomorphic markers (Ng 33, Ng 18 and Ng 10) differentiated the C. chinense and C. frutescens species, which was difficult because of high morphologic similarity between these two species and is usually evaluated at the flowering stage, while HRM profiles can be done at any plant stage. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-13363 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | eng |
| publishDate | 2022 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-133632022-11-10T15:23:11Z SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae) Caracterización molecular por SSR-HRM de germoplasma colombiano cultivado de Capsicum chinense Jacq. (Solanaceae) Lopéz-Candelo, José Enrique Viáfara-Vega, Ronald Andrés Cárdenas-Henao, Heiber Artificial selection Crop species identification and discrimination Crop genetic diversity Usefulness of single nucleotide polymorphisms Diversidad genética de cultivos Identificación y discriminación de especies cultivadas Utilidad de polimorfismos de nucleótido simple This study was the first evaluation of the genetic diversity of cultivated populations of habanero pepper (Capsicum chinense) in Colombia using SSR-HRM. Three habanero pepper lines were characterized with eight microsatellite markers using the High-Resolution Melt (HRM) technique. Twenty-seven individuals from the HL-original line and 30 individuals each from derived lines HL-70 and HL67 were genotyped. Three microsatellites were monomorphic, and five were polymorphic; however, a high allelic diversity was detected in the homozygous state in the 87 individuals. The Ng8 marker differentiated the HL-original and HL-67 lines from the HL-70 line with HRM profiles. The analysis of molecular variance (AMOVA) revelated that 52% of the genetic variation existed within lines. The HL-67 line was more similar to the HL-original line than to the HL-70 line. HL-70 recorded the highest genetic diversity for the derived lines and, therefore, could be used in a new breeding program. In contrast, the HL-67 line, because of its high genetic homogeneity, could potentially be used to evaluate different environmental conditions to find optimal conditions that increase productivity and pungency. Finally, comparing the HRM profiles with the monomorphic markers (Ng 33, Ng 18 and Ng 10) differentiated the C. chinense and C. frutescens species, which was difficult because of high morphologic similarity between these two species and is usually evaluated at the flowering stage, while HRM profiles can be done at any plant stage. Este estudio es la primera aproximación al conocimiento de la diversidad genética de poblaciones cultivadas de chile habanero (Capsicum chinense) en Colombia utilizando SSR-HRM. Se caracterizaron tres líneas de chile habanero con ocho marcadores microsatélites utilizando la técnica High-Resolution Melting (HRM). Veintisiete individuos de la línea original HL y 30 de cada línea derivada HL-70 y HL67 fueron genotipificados. Tres microsatélites resultaron monomórficos y cinco polimórficos; sin embargo, se detectó una alta diversidad alélica en el estado homocigoto en los 87 individuos evaluados. El marcador Ng8 diferenció las líneas HL-original y HL-67 de la línea HL-70 a través de sus perfiles de HRM. El análisis de varianza molecular (AMOVA) reveló que el 52% de la variación genética existía dentro de las líneas. La línea HL-67 fue más similar a la línea HL-original que a la línea HL-70, HL-70 registró la mayor diversidad genética con respecto a las líneas derivadas y, por lo tanto, podría ser considerada en un nuevo programa de mejoramiento. En contraste, la línea HL-67, debido a su alta homogeneidad genética, podría potencialmente utilizarse para evaluar diferentes condiciones ambientales en busca de condiciones óptimas para aumentar su productividad y picor. Finalmente, la comparación de los perfiles de HRM obtenidos con los marcadores monomórficos (Ng 33, Ng 18 y Ng 10) nos permitió diferenciar entre las especies C. chinense y C. frutescens, esta diferenciación es difícil debido a la alta similitud morfológica entre estas dos especies y generalmente se evalúa en la etapa de floración, mientras que los perfiles de HRM se pueden realizar en cualquier etapa de la planta. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2022-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13363 10.17584/rcch.2022v16i2.13363 Revista Colombiana de Ciencias Hortícolas; Vol. 16 No. 2 (2022); e13363 Revista Colombiana de Ciencias Hortícolas; Vol. 16 Núm. 2 (2022); e13363 Revista Colombiana de Ciencias Hortícolas; Vol. 16 No 2 (2022); e13363 Revista Colombiana de Ciencias Hortícolas; V. 16 N. 2 (2022); e13363 Revista Colombiana de Ciencias Hortícolas; v. 16 n. 2 (2022); e13363 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13363/11709 Colombia; Valle del Cauca Colombia; Valle del Cauca Copyright (c) 2022 Revista Colombiana de Ciencias Hortícolas http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Artificial selection Crop species identification and discrimination Crop genetic diversity Usefulness of single nucleotide polymorphisms Diversidad genética de cultivos Identificación y discriminación de especies cultivadas Utilidad de polimorfismos de nucleótido simple Lopéz-Candelo, José Enrique Viáfara-Vega, Ronald Andrés Cárdenas-Henao, Heiber SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae) |
| title | SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae) |
| title_alt | Caracterización molecular por SSR-HRM de germoplasma colombiano cultivado de Capsicum chinense Jacq. (Solanaceae) |
| title_full | SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae) |
| title_fullStr | SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae) |
| title_full_unstemmed | SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae) |
| title_short | SSR-HRM molecular characterization of the Colombian cultivated germplasm of Capsicum chinense Jacq. (Solanaceae) |
| title_sort | ssr hrm molecular characterization of the colombian cultivated germplasm of capsicum chinense jacq solanaceae |
| topic | Artificial selection Crop species identification and discrimination Crop genetic diversity Usefulness of single nucleotide polymorphisms Diversidad genética de cultivos Identificación y discriminación de especies cultivadas Utilidad de polimorfismos de nucleótido simple |
| topic_facet | Artificial selection Crop species identification and discrimination Crop genetic diversity Usefulness of single nucleotide polymorphisms Diversidad genética de cultivos Identificación y discriminación de especies cultivadas Utilidad de polimorfismos de nucleótido simple |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13363 |
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