Study on Croton sp. genetic diversity in the Department of Norte de Santander using the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA)

Genetic variability studies on species with a pharmacological potential are essential for conserving genetic resources. A genetic characterization of a species can guide efforts to collect and conserve germplasm for future breeding programs. The Croton genus belongs to the Euphorbiaceae family, whic...

Full description

Saved in:

Bibliographic Details
Main Author:	Chaves-Bedoya, Giovanni
Format:	Online
Language:	eng
Published:	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2022
Subjects:	Euphorbiaceae Medicinal plants Drago's blood Neotropics Genetic variability Molecular systematics Medicinal plant Euphorbiacea Plantas medicinales Sangre de Drago Neotrópico Variabilidad genética Sistemática molecular Planta medicinal
Online Access:	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13592
Tags:	Add Tag No Tags, Be the first to tag this record!

_version_	1801706303629819904
author	Chaves-Bedoya, Giovanni
author_facet	Chaves-Bedoya, Giovanni
author_sort	Chaves-Bedoya, Giovanni
collection	OJS
description	Genetic variability studies on species with a pharmacological potential are essential for conserving genetic resources. A genetic characterization of a species can guide efforts to collect and conserve germplasm for future breeding programs. The Croton genus belongs to the Euphorbiaceae family, which has approximately 1,300 species, is widely distributed in tropical and subtropical regions of the world, and has a wide range of ethnobotanical and medicinal uses. The aim of this research was to study genetic variability by analyzing sequences of the internal transcribed space region (ITS1 5'-TCCGTAGGGAACCTGCGGC-3' and ITS4 5'-TCCTCCGCTTATGC-3') of ribosomal DNA (rDNA) of a Croton population from forested areas in the Department of Norte de Santander in the municipalities of Chinacota, Pamplona and El Zulia. The results indicated considerable genetic variability in the Croton population, with a nucleotide similarity ranging from 54 to 99% and phylogenetical grouping according to the place of origin. The information gained from the ITS region can be a useful parameter for diversity evaluations and phylogenetic studies since there are no similar studies on Croton in this department in northeastern Colombia.
format	Online
id	oai:oai.revistas.uptc.edu.co:article-13592
institution	Revista Colombiana de Ciencias Hortícolas
language	eng
publishDate	2022
publisher	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
record_format	ojs
spelling	oai:oai.revistas.uptc.edu.co:article-135922022-07-11T19:23:51Z Study on Croton sp. genetic diversity in the Department of Norte de Santander using the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) Estudio de la diversidad genética de Croton sp. en el departamento de Norte de Santander utilizando la región espaciadora transcrita interna (ITS) del ADN ribosómico (rDNA) Chaves-Bedoya, Giovanni Euphorbiaceae Medicinal plants Drago's blood Neotropics Genetic variability Molecular systematics Medicinal plant Euphorbiacea Plantas medicinales Sangre de Drago Neotrópico Variabilidad genética Sistemática molecular Planta medicinal Genetic variability studies on species with a pharmacological potential are essential for conserving genetic resources. A genetic characterization of a species can guide efforts to collect and conserve germplasm for future breeding programs. The Croton genus belongs to the Euphorbiaceae family, which has approximately 1,300 species, is widely distributed in tropical and subtropical regions of the world, and has a wide range of ethnobotanical and medicinal uses. The aim of this research was to study genetic variability by analyzing sequences of the internal transcribed space region (ITS1 5'-TCCGTAGGGAACCTGCGGC-3' and ITS4 5'-TCCTCCGCTTATGC-3') of ribosomal DNA (rDNA) of a Croton population from forested areas in the Department of Norte de Santander in the municipalities of Chinacota, Pamplona and El Zulia. The results indicated considerable genetic variability in the Croton population, with a nucleotide similarity ranging from 54 to 99% and phylogenetical grouping according to the place of origin. The information gained from the ITS region can be a useful parameter for diversity evaluations and phylogenetic studies since there are no similar studies on Croton in this department in northeastern Colombia. Los estudios de variabilidad genética de especies con potencial farmacológico son esenciales para la conservación de los recursos genéticos. La caracterización genética de una especie puede guiar los esfuerzos para recolectar y conservar germoplasma para futuros programas de mejoramiento. El género Croton pertenece a la familia Euphorbiaceae, que comprende aproximadamente 1.300 especies, ampliamente distribuidas en regiones tropicales y subtropicales del mundo y se caracteriza por una amplia gama de usos a nivel etnobotánico y medicinal. El objetivo de esta investigación fue estudiar la variabilidad genética a través del análisis de secuencias de la región espaciadora transcrita interna (ITS, del inglés internal transcribed spacer) (ITS1 5'-TCCGTAGGGAACCTGCGGC-3' e ITS4 5'-TCCTCCGCTTATGC-3') del ADN ribosomal (rDNA) de una población de Croton presente en áreas boscosas del departamento de Norte de Santander en los municipios de Chinacota, Pamplona y El Zulia. Los resultados indican que existe una considerable variabilidad genética entre los individuos de la población de Croton con una similitud de nucleótidos que oscila entre el 54 y el 99%, agrupándose filogenéticamente según el lugar de origen. La información generada en este trabajo desde la región ITS es un parámetro útil para la evaluación de la diversidad y los estudios filogenéticos ya que no existen estudios de esta naturaleza en Croton en este departamento del noreste de Colombia. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2022-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13592 10.17584/rcch.2022v16i1.13592 Revista Colombiana de Ciencias Hortícolas; Vol. 16 No. 1 (2022); e13592 Revista Colombiana de Ciencias Hortícolas; Vol. 16 Núm. 1 (2022); e13592 Revista Colombiana de Ciencias Hortícolas; Vol. 16 No 1 (2022); e13592 Revista Colombiana de Ciencias Hortícolas; V. 16 N. 1 (2022); e13592 Revista Colombiana de Ciencias Hortícolas; v. 16 n. 1 (2022); e13592 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13592/11590 Colombia; Norte de Santander Colombia; Norte de Santander Copyright (c) 2022 Revista Colombiana de Ciencias Hortícolas http://creativecommons.org/licenses/by-nc-sa/4.0
spellingShingle	Euphorbiaceae Medicinal plants Drago's blood Neotropics Genetic variability Molecular systematics Medicinal plant Euphorbiacea Plantas medicinales Sangre de Drago Neotrópico Variabilidad genética Sistemática molecular Planta medicinal Chaves-Bedoya, Giovanni Study on Croton sp. genetic diversity in the Department of Norte de Santander using the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA)
title	Study on Croton sp. genetic diversity in the Department of Norte de Santander using the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA)
title_alt	Estudio de la diversidad genética de Croton sp. en el departamento de Norte de Santander utilizando la región espaciadora transcrita interna (ITS) del ADN ribosómico (rDNA)
title_full	Study on Croton sp. genetic diversity in the Department of Norte de Santander using the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA)
title_fullStr	Study on Croton sp. genetic diversity in the Department of Norte de Santander using the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA)
title_full_unstemmed	Study on Croton sp. genetic diversity in the Department of Norte de Santander using the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA)
title_short	Study on Croton sp. genetic diversity in the Department of Norte de Santander using the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA)
title_sort	study on croton sp genetic diversity in the department of norte de santander using the internal transcribed spacer its region of ribosomal dna rdna
topic	Euphorbiaceae Medicinal plants Drago's blood Neotropics Genetic variability Molecular systematics Medicinal plant Euphorbiacea Plantas medicinales Sangre de Drago Neotrópico Variabilidad genética Sistemática molecular Planta medicinal
topic_facet	Euphorbiaceae Medicinal plants Drago's blood Neotropics Genetic variability Molecular systematics Medicinal plant Euphorbiacea Plantas medicinales Sangre de Drago Neotrópico Variabilidad genética Sistemática molecular Planta medicinal
url	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13592
work_keys_str_mv	AT chavesbedoyagiovanni studyoncrotonspgeneticdiversityinthedepartmentofnortedesantanderusingtheinternaltranscribedspaceritsregionofribosomaldnardna AT chavesbedoyagiovanni estudiodeladiversidadgeneticadecrotonspeneldepartamentodenortedesantanderutilizandolaregionespaciadoratranscritainternaitsdeladnribosomicordna