Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.))

Classic plant breeding, based on the selection of superior individuals and directed crosses, led to the need-to-know aspects of the floral biology of cowpea beans. The research was carried out at the Universidad de Córdoba, Colombia, through two experiments: in the first, stigmatic receptivity was e...

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Main Authors:	Araméndiz-Tatis, Hermes, Cardona-Ayala, Carlos, Espitia-Camacho, Miguel, Hernández-Murillo, Jenry Rafael
Format:	Online
Language:	eng
Published:	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2022
Subjects:	Flower stigma Peroxidase Emasculation Anthesis Pollination Plant breeding Bean Estigma floral Peroxidasa Emasculación Antesis Polinización Mejoramiento de plantas Frijol
Online Access:	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13820
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author	Araméndiz-Tatis, Hermes Cardona-Ayala, Carlos Espitia-Camacho, Miguel Hernández-Murillo, Jenry Rafael
author_facet	Araméndiz-Tatis, Hermes Cardona-Ayala, Carlos Espitia-Camacho, Miguel Hernández-Murillo, Jenry Rafael
author_sort	Araméndiz-Tatis, Hermes
collection	OJS
description	Classic plant breeding, based on the selection of superior individuals and directed crosses, led to the need-to-know aspects of the floral biology of cowpea beans. The research was carried out at the Universidad de Córdoba, Colombia, through two experiments: in the first, stigmatic receptivity was evaluated as response time to hydrogen peroxide, under a randomized complete block design, with a 3×4 factorial arrangement (three genotypes: Caupicor 50, Missouri and BRS Milenium, and at four hours of the day: 7:00 and 9:00 AM; 3:00 and 5:00 PM) and three replications. In the second, the percentage of viable crosses was evaluated, under a randomized complete block design, with a 2×2 factorial arrangement (two crosses: Missouri × IT86 and Missouri × BRS Milenium, and two methods: 1 (morning) and 2 (afternoon), and four replications. The greatest stigmatic receptivity was recorded in the BRS Milenium and Missouri genotypes with a time of 3.28±0.07 and 2.01±0.12 min at 7:00 and 9:00 AM, while Caupicor 50, time of 1.80±0.09 min at 3:00 PM. The artificial hybridizations carried out in the morning (method 1) registered the highest viable crosses, 78.6% in Missouri × BRS Milenium and 57.1% in Missouri × IT86. Therefore, artificial hybridizations should be done in the morning due to a more favorable environment for pollen grain germination, given the greater stigmatic receptivity.
format	Online
id	oai:oai.revistas.uptc.edu.co:article-13820
institution	Revista Colombiana de Ciencias Hortícolas
language	eng
publishDate	2022
publisher	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
record_format	ojs
spelling	oai:oai.revistas.uptc.edu.co:article-138202022-11-10T15:23:11Z Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.)) Receptividad estigmática e hibridación artificial en frijol caupí (Vigna unguiculata L. (Walp.)) Araméndiz-Tatis, Hermes Cardona-Ayala, Carlos Espitia-Camacho, Miguel Hernández-Murillo, Jenry Rafael Flower stigma Peroxidase Emasculation Anthesis Pollination Plant breeding Bean Estigma floral Peroxidasa Emasculación Antesis Polinización Mejoramiento de plantas Frijol Classic plant breeding, based on the selection of superior individuals and directed crosses, led to the need-to-know aspects of the floral biology of cowpea beans. The research was carried out at the Universidad de Córdoba, Colombia, through two experiments: in the first, stigmatic receptivity was evaluated as response time to hydrogen peroxide, under a randomized complete block design, with a 3×4 factorial arrangement (three genotypes: Caupicor 50, Missouri and BRS Milenium, and at four hours of the day: 7:00 and 9:00 AM; 3:00 and 5:00 PM) and three replications. In the second, the percentage of viable crosses was evaluated, under a randomized complete block design, with a 2×2 factorial arrangement (two crosses: Missouri × IT86 and Missouri × BRS Milenium, and two methods: 1 (morning) and 2 (afternoon), and four replications. The greatest stigmatic receptivity was recorded in the BRS Milenium and Missouri genotypes with a time of 3.28±0.07 and 2.01±0.12 min at 7:00 and 9:00 AM, while Caupicor 50, time of 1.80±0.09 min at 3:00 PM. The artificial hybridizations carried out in the morning (method 1) registered the highest viable crosses, 78.6% in Missouri × BRS Milenium and 57.1% in Missouri × IT86. Therefore, artificial hybridizations should be done in the morning due to a more favorable environment for pollen grain germination, given the greater stigmatic receptivity. El mejoramiento genético clásico, basado en la selección de individuos superiores y cruzamientos dirigidos, demanda conocer aspectos de la biología floral y reproductiva del fríjol caupí. La investigación se llevó a cabo en la Universidad de Córdoba, mediante dos experimentos: en el primero, se evaluó la receptividad estigmática como tiempo de respuesta al peróxido de hidrógeno, bajo el diseño bloques completos aleatorizados con arreglo factorial 3×4 (tres genotipos: Caupicor 50, Missouri y BRS Milenium, y cuatro horas del día: 7:00 y 9:00 AM, 3:00 y 5:00 PM) y tres repeticiones; en el segundo, se evaluó el porcentaje de cruzamientos viables, bajo un diseño Bloques Completos al azar con arreglo factorial 2×2 (dos cruzamientos: Missouri × IT86 y Missouri × BRS Milenium, dos métodos: 1 (mañana) y 2 (tarde), y cuatro repeticiones. La mayor receptividad estigmática se registró en los genotipos BRS Milenium y Missouri, con tiempo de 3,28±0,07 y 2,01±0,12 min a las 7:00 y 9:00 AM, mientras que Caupicor 50, tiempo de 1,80±0,09 min a las 3:00 AM. Las hibridaciones artificiales realizadas por la mañana registraron los mayores cruzamientos viables, 78,6% en Missouri × BRS Milenium y 57,1% en Missouri × IT86. Por tanto, las hibridaciones artificiales deben hacerse en horas de la mañana debido a un ambiente más propicio para la germinación del grano de polen, dada la mayor receptividad estigmática. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2022-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13820 10.17584/rcch.2022v16i2.13820 Revista Colombiana de Ciencias Hortícolas; Vol. 16 No. 2 (2022); e13820 Revista Colombiana de Ciencias Hortícolas; Vol. 16 Núm. 2 (2022); e13820 Revista Colombiana de Ciencias Hortícolas; Vol. 16 No 2 (2022); e13820 Revista Colombiana de Ciencias Hortícolas; V. 16 N. 2 (2022); e13820 Revista Colombiana de Ciencias Hortícolas; v. 16 n. 2 (2022); e13820 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13820/11720 Colombia; Cordoba; Monteria Colombia; Cordoba; Monteria Copyright (c) 2022 Revista Colombiana de Ciencias Hortícolas http://creativecommons.org/licenses/by-nc-sa/4.0
spellingShingle	Flower stigma Peroxidase Emasculation Anthesis Pollination Plant breeding Bean Estigma floral Peroxidasa Emasculación Antesis Polinización Mejoramiento de plantas Frijol Araméndiz-Tatis, Hermes Cardona-Ayala, Carlos Espitia-Camacho, Miguel Hernández-Murillo, Jenry Rafael Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.))
title	Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.))
title_alt	Receptividad estigmática e hibridación artificial en frijol caupí (Vigna unguiculata L. (Walp.))
title_full	Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.))
title_fullStr	Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.))
title_full_unstemmed	Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.))
title_short	Stigmatic receptivity and hybridization in cowpea beans (Vigna unguiculata L. (Walp.))
title_sort	stigmatic receptivity and hybridization in cowpea beans vigna unguiculata l walp
topic	Flower stigma Peroxidase Emasculation Anthesis Pollination Plant breeding Bean Estigma floral Peroxidasa Emasculación Antesis Polinización Mejoramiento de plantas Frijol
topic_facet	Flower stigma Peroxidase Emasculation Anthesis Pollination Plant breeding Bean Estigma floral Peroxidasa Emasculación Antesis Polinización Mejoramiento de plantas Frijol
url	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/13820
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