Persistence of Bacillus thuringiensis and Bacillus pumilus potential biological control agents of the coffee berry borer under field conditions of Puerto Rico
Field trials were conducted in two years to evaluate persistence of B. thuringiensis and Bacillus pumilus under field conditions. Strains of B. thuringiensis (3971) and B. pumilus (4185) were isolated from coffee agro-ecosystem in Puerto Rico. Field trials consisted in plots of nine coffee trees arr...
| Main Authors: | , |
|---|---|
| Format: | Online |
| Language: | spa |
| Published: |
Universidad Pedagógica y Tecnológica de Colombia
2022
|
| Subjects: | |
| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/14685 |
| _version_ | 1801706483263471616 |
|---|---|
| author | Bolanos Carriel, Carlos Vazquez Soto, Dainette |
| author_facet | Bolanos Carriel, Carlos Vazquez Soto, Dainette |
| author_sort | Bolanos Carriel, Carlos |
| collection | OJS |
| description | Field trials were conducted in two years to evaluate persistence of B. thuringiensis and Bacillus pumilus under field conditions. Strains of B. thuringiensis (3971) and B. pumilus (4185) were isolated from coffee agro-ecosystem in Puerto Rico. Field trials consisted in plots of nine coffee trees arranged in a randomized complete block design, sprayed with Bt-3971 and Bp-4185 at 108, 109, or 1010 colony forming units CFU ml-1 or non-inoculated. In year 1, B. thuringiensis and B. pumilus survived up to six months after inoculation. Monitoring carried out during year 2, re-confirmed the survival of Bt-3971 and Bp-4185 inoculated in coffee trees under field conditions. Overall, Bt-3971 had significantly more recovery rates than Bp-4185. However, the higher bacterial recoveries were 1.24x1013 CFU ml-1 in coffee trees treated with Bt-3971 and 2.48x1013 CFU ml-1 in coffee trees treated with Bp-4185 in year 1 and year 2, respectively, both sprayed with 1010 spores ml-1. B. thuringiensis (3971) and B. pumilus (4185) isolated from coffee fields of Puerto Rico are well adapted to the environmental conditions of the island, and are valuable biological control agents against coffee berry borer. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-14685 |
| institution | Revista Ciencia y Agricultura |
| language | spa |
| publishDate | 2022 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-146852023-01-17T15:13:30Z Persistence of Bacillus thuringiensis and Bacillus pumilus potential biological control agents of the coffee berry borer under field conditions of Puerto Rico Persistencia De Cepas De Bacillus thuringiensis Y Bacillus pumilus Potenciales Controladoras Biológicas De La Broca Del Café En Condiciones de Campo en Puerto Rico Bolanos Carriel, Carlos Vazquez Soto, Dainette Coffee Berry Borer Bacillus thuringiensis Bacillus pumilus Colony forming Units Barrenador del fruto del café Bacillus thuringiensis Bacillus pumilus Unidades formadoras de colonias Field trials were conducted in two years to evaluate persistence of B. thuringiensis and Bacillus pumilus under field conditions. Strains of B. thuringiensis (3971) and B. pumilus (4185) were isolated from coffee agro-ecosystem in Puerto Rico. Field trials consisted in plots of nine coffee trees arranged in a randomized complete block design, sprayed with Bt-3971 and Bp-4185 at 108, 109, or 1010 colony forming units CFU ml-1 or non-inoculated. In year 1, B. thuringiensis and B. pumilus survived up to six months after inoculation. Monitoring carried out during year 2, re-confirmed the survival of Bt-3971 and Bp-4185 inoculated in coffee trees under field conditions. Overall, Bt-3971 had significantly more recovery rates than Bp-4185. However, the higher bacterial recoveries were 1.24x1013 CFU ml-1 in coffee trees treated with Bt-3971 and 2.48x1013 CFU ml-1 in coffee trees treated with Bp-4185 in year 1 and year 2, respectively, both sprayed with 1010 spores ml-1. B. thuringiensis (3971) and B. pumilus (4185) isolated from coffee fields of Puerto Rico are well adapted to the environmental conditions of the island, and are valuable biological control agents against coffee berry borer. Ensayos de campo se llevaron a cabo en dos años para evaluar la persistencia de Bacillus thuringiensis y Bacillus pumilus. Las cepas B. thuringiensis (3971) y B. pumilus (4185) fueron aisladas del agro-ecosistema del cafeto en Puerto Rico. Los ensayos de campo consistieron en parcelas de nueve cafetos en diseño de bloques completamente aleatorizado. Los tratamientos fueron Bt-3971 y Bp-4185 a las dosis de 108, 109 y 1010 unidades formadoras de colonias por mililitro (UFC ml-1) y parcelas no inoculadas. En el año 1, B. thuringiensis y B. pumilus sobrevivieron hasta seis meses después de la inoculación (Marzo a Septiembre). Los monitoreos realizados de Marzo a Septiembre del año 2 , re-confirmaron la sobrevivencia de Bt-3971 y Bp-4185 bajo condiciones de campo en las unidades experimentales. En general, se recuperaron más aislados de los cafetos tratados con Bt-3971 comparado con Bp-4185. Sin embargo, las tasas de recuperación de aislados más altas fueron 1.24 x 1013 UFC ml-1 en cafetos tratados con Bt-3971, y 2.48 x 1013 UFC ml-1 en cafetos tratados con Bp-4185 en los dos años respectivamente; ambos de cafetos tratados con la mayor concentración de esporas (1010 UFC ml-1). B. thuringiensis (3971) y B. pumilus (4185) aisladas del agro-ecosistema de cafeto en Puerto Rico están bien adaptadas a las condiciones de la isla, y son valiosas cepas para el control biológico de la broca del cafeto. Universidad Pedagógica y Tecnológica de Colombia 2022-12-12 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion text texto application/pdf https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/14685 10.19053/01228420.v19.n3.2022.14685 Ciencia y Agricultura; Vol. 19 No. 3 (2022): Septiembre-Diciembre Ciencia y Agricultura; Vol. 19 Núm. 3 (2022): Septiembre-Diciembre 2539-0899 spa https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/14685/12421 Copyright (c) 2022 carlos bolanos carriel http://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Coffee Berry Borer Bacillus thuringiensis Bacillus pumilus Colony forming Units Barrenador del fruto del café Bacillus thuringiensis Bacillus pumilus Unidades formadoras de colonias Bolanos Carriel, Carlos Vazquez Soto, Dainette Persistence of Bacillus thuringiensis and Bacillus pumilus potential biological control agents of the coffee berry borer under field conditions of Puerto Rico |
| title | Persistence of Bacillus thuringiensis and Bacillus pumilus potential biological control agents of the coffee berry borer under field conditions of Puerto Rico |
| title_alt | Persistencia De Cepas De Bacillus thuringiensis Y Bacillus pumilus Potenciales Controladoras Biológicas De La Broca Del Café En Condiciones de Campo en Puerto Rico |
| title_full | Persistence of Bacillus thuringiensis and Bacillus pumilus potential biological control agents of the coffee berry borer under field conditions of Puerto Rico |
| title_fullStr | Persistence of Bacillus thuringiensis and Bacillus pumilus potential biological control agents of the coffee berry borer under field conditions of Puerto Rico |
| title_full_unstemmed | Persistence of Bacillus thuringiensis and Bacillus pumilus potential biological control agents of the coffee berry borer under field conditions of Puerto Rico |
| title_short | Persistence of Bacillus thuringiensis and Bacillus pumilus potential biological control agents of the coffee berry borer under field conditions of Puerto Rico |
| title_sort | persistence of bacillus thuringiensis and bacillus pumilus potential biological control agents of the coffee berry borer under field conditions of puerto rico |
| topic | Coffee Berry Borer Bacillus thuringiensis Bacillus pumilus Colony forming Units Barrenador del fruto del café Bacillus thuringiensis Bacillus pumilus Unidades formadoras de colonias |
| topic_facet | Coffee Berry Borer Bacillus thuringiensis Bacillus pumilus Colony forming Units Barrenador del fruto del café Bacillus thuringiensis Bacillus pumilus Unidades formadoras de colonias |
| url | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/14685 |
| work_keys_str_mv | AT bolanoscarrielcarlos persistenceofbacillusthuringiensisandbacilluspumiluspotentialbiologicalcontrolagentsofthecoffeeberryborerunderfieldconditionsofpuertorico AT vazquezsotodainette persistenceofbacillusthuringiensisandbacilluspumiluspotentialbiologicalcontrolagentsofthecoffeeberryborerunderfieldconditionsofpuertorico AT bolanoscarrielcarlos persistenciadecepasdebacillusthuringiensisybacilluspumiluspotencialescontroladorasbiologicasdelabrocadelcafeencondicionesdecampoenpuertorico AT vazquezsotodainette persistenciadecepasdebacillusthuringiensisybacilluspumiluspotencialescontroladorasbiologicasdelabrocadelcafeencondicionesdecampoenpuertorico |
