Bio-catalytic oxidation of aromatic pollutants caused by peroxidase from soybean
This paper describes the biocatalytic performance of soybean peroxidase enzyme to oxidize aqueous aromatic pollutants such as organophosphorus pesticides and polycyclic aromatic hydrocarbons (PAHs), and flavonoids from agro-industrial wastes. The conversions achieved were for the pesticides terbufos...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia - UPTC
2022
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| Subjects: | |
| Online Access: | https://revistas.uptc.edu.co/index.php/ingenieria_sogamoso/article/view/15022 |
| _version_ | 1801706697789538304 |
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| author | Hernández Hernández, María Ixel Romero Guido, Cynthia Torres Ramírez, Eduardo |
| author_facet | Hernández Hernández, María Ixel Romero Guido, Cynthia Torres Ramírez, Eduardo |
| author_sort | Hernández Hernández, María Ixel |
| collection | OJS |
| description | This paper describes the biocatalytic performance of soybean peroxidase enzyme to oxidize aqueous aromatic pollutants such as organophosphorus pesticides and polycyclic aromatic hydrocarbons (PAHs), and flavonoids from agro-industrial wastes. The conversions achieved were for the pesticides terbufos (14%) and dimethoate (30% conversion); for HPAs such as azulene (32%), 9-methyl anthracene (22%); anthracene, pyrene (29% each), and carbazole (18%); and for flavonoids such as ferulic acid (99%), quercetin (98%), rutin (50%) and naringin (10%). The tests were carried out on model samples and on simulated samples of contaminated effluents. In addition, the effect of pH and temperature on the enzyme activity was studied, being active from pH 3 to 6, and from 25 ˚C to 80 ˚C. The above concludes that soybean peroxidase is a good candidate for more comprehensive studies for possible application in effluents treatment containing aromatic pollutants |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-15022 |
| institution | Revista Ingeniería, Investigación y Desarrollo |
| language | spa |
| publishDate | 2022 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia - UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-150222023-06-25T18:59:23Z Bio-catalytic oxidation of aromatic pollutants caused by peroxidase from soybean OXIDACIÓN BIOCATALÍTICA DE CONTAMINANTES AROMÁTICOS POR LA PEROXIDASA PROVENIENTE DE LA SOYA Hernández Hernández, María Ixel Romero Guido, Cynthia Torres Ramírez, Eduardo biocatalysis aromatic pollutants soybean peroxidase biocatálisis contaminantes aromáticos peroxidasa de soya This paper describes the biocatalytic performance of soybean peroxidase enzyme to oxidize aqueous aromatic pollutants such as organophosphorus pesticides and polycyclic aromatic hydrocarbons (PAHs), and flavonoids from agro-industrial wastes. The conversions achieved were for the pesticides terbufos (14%) and dimethoate (30% conversion); for HPAs such as azulene (32%), 9-methyl anthracene (22%); anthracene, pyrene (29% each), and carbazole (18%); and for flavonoids such as ferulic acid (99%), quercetin (98%), rutin (50%) and naringin (10%). The tests were carried out on model samples and on simulated samples of contaminated effluents. In addition, the effect of pH and temperature on the enzyme activity was studied, being active from pH 3 to 6, and from 25 ˚C to 80 ˚C. The above concludes that soybean peroxidase is a good candidate for more comprehensive studies for possible application in effluents treatment containing aromatic pollutants El presente artículo describe el desempeño biocatalítico de la enzima peroxidasa de la soya para oxidar contaminantes acuosos del tipo aromáticos como plaguicidas organofosforados, hidrocarburos policíclicos aromáticos (HPAs), y flavonoides provenientes de residuos agroindustriales. Los ensayos se realizaron en muestras modelo y también en muestras simuladas de efluentes contaminados. El análisis se realizó por cromatografía de líquidos de alta resolución (HPLC) y por espectrofotometría UV-vis. Las conversiones alcanzadas fueron para los plaguicidas terbufos (14%) y dimetoato (30% de conversión); para los HPAs como azuleno (32%), 9-metil antraceno (22%); antraceno y pireno (29% cada uno), y carbazol (18%); y los flavonoides como ácido ferúlico (99%), quercetina (98%), rutina (50%) y naringina (10%). Se estudió el efecto del cambio de pH y de temperatura en la actividad de la enzima, siendo activa desde un pH de 3 hasta 6, y desde los 25 ˚C hasta los 80 ˚C. Lo anterior permite concluir que la peroxidasa de soya es un buen candidato para estudios más completos para su posible aplicación en la descontaminación de efluentes que contienen contaminantes aromáticos. Universidad Pedagógica y Tecnológica de Colombia - UPTC 2022-09-29 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ingenieria_sogamoso/article/view/15022 10.19053/1900771X.v22.n2.2022.15022 Ingeniería Investigación y Desarrollo; Vol. 22 No. 2 (2022): Julio - Diciembre; 44-50 Ingeniería Investigación y Desarrollo; Vol. 22 Núm. 2 (2022): Julio - Diciembre; 44-50 2422-4324 1900-771X spa https://revistas.uptc.edu.co/index.php/ingenieria_sogamoso/article/view/15022/12236 |
| spellingShingle | biocatalysis aromatic pollutants soybean peroxidase biocatálisis contaminantes aromáticos peroxidasa de soya Hernández Hernández, María Ixel Romero Guido, Cynthia Torres Ramírez, Eduardo Bio-catalytic oxidation of aromatic pollutants caused by peroxidase from soybean |
| title | Bio-catalytic oxidation of aromatic pollutants caused by peroxidase from soybean |
| title_alt | OXIDACIÓN BIOCATALÍTICA DE CONTAMINANTES AROMÁTICOS POR LA PEROXIDASA PROVENIENTE DE LA SOYA |
| title_full | Bio-catalytic oxidation of aromatic pollutants caused by peroxidase from soybean |
| title_fullStr | Bio-catalytic oxidation of aromatic pollutants caused by peroxidase from soybean |
| title_full_unstemmed | Bio-catalytic oxidation of aromatic pollutants caused by peroxidase from soybean |
| title_short | Bio-catalytic oxidation of aromatic pollutants caused by peroxidase from soybean |
| title_sort | bio catalytic oxidation of aromatic pollutants caused by peroxidase from soybean |
| topic | biocatalysis aromatic pollutants soybean peroxidase biocatálisis contaminantes aromáticos peroxidasa de soya |
| topic_facet | biocatalysis aromatic pollutants soybean peroxidase biocatálisis contaminantes aromáticos peroxidasa de soya |
| url | https://revistas.uptc.edu.co/index.php/ingenieria_sogamoso/article/view/15022 |
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