ANTIOXIDANT ACTIVITY OF NON-PSYCHOACTIVE CANNABIS VARIETIES FROM NORTH CAUCA, COLOMBIA
The present study aim to publicize the chemical composition and possible medicinal potential of varieties of non-psychoactive cannabis grown in the department of Cauca. Cannabinoids were identified and quantified by high-performance liquid chromatography coupled to an ultraviolet detector (HPLC/UV)...
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| Format: | Online |
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Universidad Pedagógica y Tecnológica de Colombia
2024
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15214 |
| _version_ | 1801706356962492416 |
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| author | Arango Romero, Paola Andrea Delgado, Jhan Carlos Muñoz, Sandra Marcela Llanos, Luis Benítez, Ricardo |
| author_facet | Arango Romero, Paola Andrea Delgado, Jhan Carlos Muñoz, Sandra Marcela Llanos, Luis Benítez, Ricardo |
| author_sort | Arango Romero, Paola Andrea |
| collection | OJS |
| description | The present study aim to publicize the chemical composition and possible medicinal potential of varieties of non-psychoactive cannabis grown in the department of Cauca. Cannabinoids were identified and quantified by high-performance liquid chromatography coupled to an ultraviolet detector (HPLC/UV) for the analysis of the flower, and gas chromatography coupled to a mass spectrometer (GC-MS) for the analysis of the ethanolic extracts and content terpenes. Phenols were quantified by reaction with the Folin & Ciocalteau reagent; for the determination of flavonoids and anthraquinones, the extracts were treated with AlCl3. Finally, to determine the antioxidant activity, three methods were used: DPPH, ABTS and FRAP. It was possible to determine that varieties A and B contained percentages of total tetrahydrocannabinol (THC) less than 1% and percentages of total cannabidiol (CBD) between 9-15%. In the ethanolic extracts concentrations (m/m) of CBD were reached in varieties A and B, of 10% and 13.7%, respectively. Nine terpenes from sample A and seven from sample B were identified and quantified, with β-caryophyllene being the most abundant in both. Considering that there is evidence in the literature that the CBD/THC ratio influences biological activity, ethanolic extracts of varieties A and B are expected to have moderate to low antioxidant activity, which, according to some researchers, it may be associated with the neuroprotective effect, which may be favored by the presence of β-caryophyllene. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-15214 |
| institution | Revista Ciencia en Desarrollo |
| publishDate | 2024 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-152142024-05-11T07:17:20Z ANTIOXIDANT ACTIVITY OF NON-PSYCHOACTIVE CANNABIS VARIETIES FROM NORTH CAUCA, COLOMBIA Arango Romero, Paola Andrea Delgado, Jhan Carlos Muñoz, Sandra Marcela Llanos, Luis Benítez, Ricardo Actividad antioxidante Cannabinoides Cannabis Terpenos Antioxidant activity Cannabinoid Cannabis Terpenoid The present study aim to publicize the chemical composition and possible medicinal potential of varieties of non-psychoactive cannabis grown in the department of Cauca. Cannabinoids were identified and quantified by high-performance liquid chromatography coupled to an ultraviolet detector (HPLC/UV) for the analysis of the flower, and gas chromatography coupled to a mass spectrometer (GC-MS) for the analysis of the ethanolic extracts and content terpenes. Phenols were quantified by reaction with the Folin & Ciocalteau reagent; for the determination of flavonoids and anthraquinones, the extracts were treated with AlCl3. Finally, to determine the antioxidant activity, three methods were used: DPPH, ABTS and FRAP. It was possible to determine that varieties A and B contained percentages of total tetrahydrocannabinol (THC) less than 1% and percentages of total cannabidiol (CBD) between 9-15%. In the ethanolic extracts concentrations (m/m) of CBD were reached in varieties A and B, of 10% and 13.7%, respectively. Nine terpenes from sample A and seven from sample B were identified and quantified, with β-caryophyllene being the most abundant in both. Considering that there is evidence in the literature that the CBD/THC ratio influences biological activity, ethanolic extracts of varieties A and B are expected to have moderate to low antioxidant activity, which, according to some researchers, it may be associated with the neuroprotective effect, which may be favored by the presence of β-caryophyllene. El presente estudio tiene como objetivo dar a conocer la composición química y el posible potencial medicinal de variedades de cannabis no psicoactivo cultivadas en el departamento del Cauca. Los cannabinoides fueron identificados y cuantificados por cromatografía líquida de alta resolución acoplada a un detector ultravioleta (HPLC/UV) para el análisis de la flor, y cromatografía de gases acoplada a un espectrómetro de masas (GC-MS) para el análisis de los extractos etanólicos y contenido terpenos. Los fenoles se cuantificaron por reacción con el reactivo de Folin & Ciocalteau; para la determinación de flavonoides y antraquinonas, los extractos fueron tratados con AlCl3. Finalmente, para determinar la actividad antioxidante se utilizaron tres métodos: DPPH, ABTS y FRAP. Se pudo determinar que las variedades A y B contenían porcentajes de tetrahidrocannabinol total (THC) menores al 1% y porcentajes de cannabidiol total (CBD) entre 9-15%. En los extractos etanólicos se alcanzaron concentraciones (m/m) de CBD en las variedades A y B, del 10% y 13,7%, respectivamente. Se identificaron y cuantificaron nueve terpenos de la muestra A y siete de la muestra B, siendo el β-cariofileno el más abundante en ambos. Teniendo en cuenta que existe evidencia en la literatura de que la relación CBD/THC influye en la actividad biológica, se espera que los extractos etanólicos de las variedades A y B tengan una actividad antioxidante de moderada a baja, lo que, según algunos investigadores, puede estar asociado con el efecto neuroprotector, que puede verse favorecido por la presencia de β-cariofileno. Universidad Pedagógica y Tecnológica de Colombia 2024-04-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion "Antioxidant activity of Cannabis varieties from Cauca - Colombia" "Actividad antioxidante de variedades de Cannabis del Cauca - Colombia" https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15214 10.19053/01217488.v15.n1.2024.15214 Ciencia En Desarrollo; Vol. 15 No. 1 (2024): Vol 15, Núm.1 (2024): Enero-Junio Ciencia en Desarrollo; Vol. 15 Núm. 1 (2024): Vol 15, Núm.1 (2024): Enero-Junio 2462-7658 0121-7488 |
| spellingShingle | Actividad antioxidante Cannabinoides Cannabis Terpenos Antioxidant activity Cannabinoid Cannabis Terpenoid Arango Romero, Paola Andrea Delgado, Jhan Carlos Muñoz, Sandra Marcela Llanos, Luis Benítez, Ricardo ANTIOXIDANT ACTIVITY OF NON-PSYCHOACTIVE CANNABIS VARIETIES FROM NORTH CAUCA, COLOMBIA |
| title | ANTIOXIDANT ACTIVITY OF NON-PSYCHOACTIVE CANNABIS VARIETIES FROM NORTH CAUCA, COLOMBIA |
| title_full | ANTIOXIDANT ACTIVITY OF NON-PSYCHOACTIVE CANNABIS VARIETIES FROM NORTH CAUCA, COLOMBIA |
| title_fullStr | ANTIOXIDANT ACTIVITY OF NON-PSYCHOACTIVE CANNABIS VARIETIES FROM NORTH CAUCA, COLOMBIA |
| title_full_unstemmed | ANTIOXIDANT ACTIVITY OF NON-PSYCHOACTIVE CANNABIS VARIETIES FROM NORTH CAUCA, COLOMBIA |
| title_short | ANTIOXIDANT ACTIVITY OF NON-PSYCHOACTIVE CANNABIS VARIETIES FROM NORTH CAUCA, COLOMBIA |
| title_sort | antioxidant activity of non psychoactive cannabis varieties from north cauca colombia |
| topic | Actividad antioxidante Cannabinoides Cannabis Terpenos Antioxidant activity Cannabinoid Cannabis Terpenoid |
| topic_facet | Actividad antioxidante Cannabinoides Cannabis Terpenos Antioxidant activity Cannabinoid Cannabis Terpenoid |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15214 |
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