| Summary: | Cell cultures are a tool that allows the maintenance of cells in vitro preserving their physiological, biochemical and genetic characteristics to the maximum, which can present very diverse properties depending on the species, tissue of origin and the culture medium used. The use of dipteran cell cultures constitutes a valuable methodology in different disciplines, such as physiology, genetics, biochemistry and pathology; thus, for example, it has been possible to study various diseases of interest in both human and veterinary medicine. This review presents information on multiple cell lines of the order Diptera, which have been used to carry out studies of cell-cell interaction, intracellular flow of metabolites, production of bioinsecticides, viral amplification and production of antimicrobial peptides. In Colombia, 12 dipteran cell lines have been established, characterized and standardized, some of which have been used as substrates in studies of the biological cycle of parasites of the genus Leishmania, as well as in the evaluation of arbovirus susceptibility. In this trend, the information on cell lines derived from dipterans is analyzed and discussed in order to carry out this review of their characteristics and applications.
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