Diptera Cell Lines: Characteristics, Applications and Contributions From Colombia
Cell cultures are a tool that allows the maintenance of cells in vitro preserving their physiological, biochemical and genetic characteristics to the maximum, which can present very diverse properties depending on the species, tissue of origin and the culture medium used. The use of dipteran cell...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Online |
| Language: | spa |
| Published: |
Universidad Pedagógica y Tecnológica de Colombia
2023
|
| Subjects: | |
| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15221 |
| _version_ | 1801706357225684992 |
|---|---|
| author | Jiménez Camacho , Ingrid Dayana Losada, Monica Rangel, Hector Rodriguez, Anny Karely Muñoz, Ana Luisa Bello, Felio Jesus Segura Guerrero, Nidya Alexandra |
| author_facet | Jiménez Camacho , Ingrid Dayana Losada, Monica Rangel, Hector Rodriguez, Anny Karely Muñoz, Ana Luisa Bello, Felio Jesus Segura Guerrero, Nidya Alexandra |
| author_sort | Jiménez Camacho , Ingrid Dayana |
| collection | OJS |
| description |
Cell cultures are a tool that allows the maintenance of cells in vitro preserving their physiological, biochemical and genetic characteristics to the maximum, which can present very diverse properties depending on the species, tissue of origin and the culture medium used. The use of dipteran cell cultures constitutes a valuable methodology in different disciplines, such as physiology, genetics, biochemistry and pathology; thus, for example, it has been possible to study various diseases of interest in both human and veterinary medicine. This review presents information on multiple cell lines of the order Diptera, which have been used to carry out studies of cell-cell interaction, intracellular flow of metabolites, production of bioinsecticides, viral amplification and production of antimicrobial peptides. In Colombia, 12 dipteran cell lines have been established, characterized and standardized, some of which have been used as substrates in studies of the biological cycle of parasites of the genus Leishmania, as well as in the evaluation of arbovirus susceptibility. In this trend, the information on cell lines derived from dipterans is analyzed and discussed in order to carry out this review of their characteristics and applications.
|
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-15221 |
| institution | Revista Ciencia en Desarrollo |
| language | spa |
| publishDate | 2023 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-152212024-05-10T00:51:44Z Diptera Cell Lines: Characteristics, Applications and Contributions From Colombia Líneas celulares de dípteros: características, aplicaciones y aportes desde Colombia Jiménez Camacho , Ingrid Dayana Losada, Monica Rangel, Hector Rodriguez, Anny Karely Muñoz, Ana Luisa Bello, Felio Jesus Segura Guerrero, Nidya Alexandra Biotecnología cultivos celulares Diptera susceptibilidad viral Biotechnology cell culture Diptera susceptibility viral susceptibility Cell cultures are a tool that allows the maintenance of cells in vitro preserving their physiological, biochemical and genetic characteristics to the maximum, which can present very diverse properties depending on the species, tissue of origin and the culture medium used. The use of dipteran cell cultures constitutes a valuable methodology in different disciplines, such as physiology, genetics, biochemistry and pathology; thus, for example, it has been possible to study various diseases of interest in both human and veterinary medicine. This review presents information on multiple cell lines of the order Diptera, which have been used to carry out studies of cell-cell interaction, intracellular flow of metabolites, production of bioinsecticides, viral amplification and production of antimicrobial peptides. In Colombia, 12 dipteran cell lines have been established, characterized and standardized, some of which have been used as substrates in studies of the biological cycle of parasites of the genus Leishmania, as well as in the evaluation of arbovirus susceptibility. In this trend, the information on cell lines derived from dipterans is analyzed and discussed in order to carry out this review of their characteristics and applications. Los cultivos celulares son una herramienta que permite el mantenimiento de células in vitro conservando al máximo sus características fisiológicas, bioquímicas y genéticas, los cuales pueden presentar propiedades muy diversas dependiendo de la especie, tejido de origen y el medio de cultivo empleado. El uso de cultivos celulares de dípteros constituye una metodología valiosa en diferentes disciplinas, tales como fisiología, genética, bioquímica y patología; así, por ejemplo, se han podido estudiar diversas enfermedades tanto de interés en medicina humana como en veterinaria. En la presente revisión se presenta información de múltiples líneas celulares del orden Diptera, las cuales se han empleado para llevar a cabo estudios de interacción célula a célula, flujo intracelular de metabolitos, producción de bioinsecticidas, amplificación viral y producción de péptidos antimicrobiano. En Colombia, se han establecido 12 líneas celulares de dípteros las cuales fueron caracterizadas y estandarizadas, algunas de ellas se han empleado como sustratos en estudios del ciclo biológico de parásitos del género Leishmania, también, en la evaluación a la susceptibilidad de arbovirus. En esta tendencia, se analiza y discute la información de las líneas celulares derivadas de dípteros con el fin de realizar el presente trabajo de revisión sobre sus características y aplicaciones. Universidad Pedagógica y Tecnológica de Colombia 2023-07-19 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15221 10.19053/01217488.v14.n2.2023.15221 Ciencia En Desarrollo; Vol. 14 No. 2 (2023): Vol 14, Núm.2 (2023): Julio-Diciembre; 47-67 Ciencia en Desarrollo; Vol. 14 Núm. 2 (2023): Vol 14, Núm.2 (2023): Julio-Diciembre; 47-67 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15221/13674 |
| spellingShingle | Biotecnología cultivos celulares Diptera susceptibilidad viral Biotechnology cell culture Diptera susceptibility viral susceptibility Jiménez Camacho , Ingrid Dayana Losada, Monica Rangel, Hector Rodriguez, Anny Karely Muñoz, Ana Luisa Bello, Felio Jesus Segura Guerrero, Nidya Alexandra Diptera Cell Lines: Characteristics, Applications and Contributions From Colombia |
| title | Diptera Cell Lines: Characteristics, Applications and Contributions From Colombia |
| title_alt | Líneas celulares de dípteros: características, aplicaciones y aportes desde Colombia |
| title_full | Diptera Cell Lines: Characteristics, Applications and Contributions From Colombia |
| title_fullStr | Diptera Cell Lines: Characteristics, Applications and Contributions From Colombia |
| title_full_unstemmed | Diptera Cell Lines: Characteristics, Applications and Contributions From Colombia |
| title_short | Diptera Cell Lines: Characteristics, Applications and Contributions From Colombia |
| title_sort | diptera cell lines characteristics applications and contributions from colombia |
| topic | Biotecnología cultivos celulares Diptera susceptibilidad viral Biotechnology cell culture Diptera susceptibility viral susceptibility |
| topic_facet | Biotecnología cultivos celulares Diptera susceptibilidad viral Biotechnology cell culture Diptera susceptibility viral susceptibility |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15221 |
| work_keys_str_mv | AT jimenezcamachoingriddayana dipteracelllinescharacteristicsapplicationsandcontributionsfromcolombia AT losadamonica dipteracelllinescharacteristicsapplicationsandcontributionsfromcolombia AT rangelhector dipteracelllinescharacteristicsapplicationsandcontributionsfromcolombia AT rodriguezannykarely dipteracelllinescharacteristicsapplicationsandcontributionsfromcolombia AT munozanaluisa dipteracelllinescharacteristicsapplicationsandcontributionsfromcolombia AT bellofeliojesus dipteracelllinescharacteristicsapplicationsandcontributionsfromcolombia AT seguraguerreronidyaalexandra dipteracelllinescharacteristicsapplicationsandcontributionsfromcolombia AT jimenezcamachoingriddayana lineascelularesdedipteroscaracteristicasaplicacionesyaportesdesdecolombia AT losadamonica lineascelularesdedipteroscaracteristicasaplicacionesyaportesdesdecolombia AT rangelhector lineascelularesdedipteroscaracteristicasaplicacionesyaportesdesdecolombia AT rodriguezannykarely lineascelularesdedipteroscaracteristicasaplicacionesyaportesdesdecolombia AT munozanaluisa lineascelularesdedipteroscaracteristicasaplicacionesyaportesdesdecolombia AT bellofeliojesus lineascelularesdedipteroscaracteristicasaplicacionesyaportesdesdecolombia AT seguraguerreronidyaalexandra lineascelularesdedipteroscaracteristicasaplicacionesyaportesdesdecolombia |
