Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática

Cannabis sativa is a widely used plant in Colombia with medicinal purposes, for which its flower is used, generating high amounts of waste stems. In this study, Cannabis stems were pretreated with diluted sulfuric acid and sodium hydroxide and its effect was analyzed by infrared spectroscopy (FTIR-A...

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Main Authors: Pérez Aguilar, Juan Alejandro, Benítez Benítez, Ricardo
Format: Online
Language:Español
Published: Universidad Pedagógica y Tecnológica de Colombia 2024
Subjects:
Online Access:https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15304
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author Pérez Aguilar, Juan Alejandro
Benítez Benítez, Ricardo
author_facet Pérez Aguilar, Juan Alejandro
Benítez Benítez, Ricardo
author_sort Pérez Aguilar, Juan Alejandro
collection OJS
description Cannabis sativa is a widely used plant in Colombia with medicinal purposes, for which its flower is used, generating high amounts of waste stems. In this study, Cannabis stems were pretreated with diluted sulfuric acid and sodium hydroxide and its effect was analyzed by infrared spectroscopy (FTIR-ATR). Remaining solids were submitted to enzymatic hydrolysis using commercial enzymatic mixtures: Celluclast 1.5 L and Cellic CTec3; production of reducing sugars was followed. Pretreatments removed hemicellulose and lignin, as evidence by the reduction in signals at 1734, 1540 and 1240 cm-1. Increase in cellulose crystallinity was also occasioned. Both pretreatments managed to increase reducing sugars production with the two enzymes used. Although, alkaline pretreatment was much more effective than acid pretreatment and allowed to produce up to 28.59 mg/mL. of reducing sugars with Celluclast 1.5 L and 24.94 mg/mL with Cellic CTec3. Mixing these two enzymes, using them sequentially, or increasing enzyme loading did not have and effect on the reached final concentration of reducing sugars.
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spelling oai:oai.revistas.uptc.edu.co:article-153042024-05-11T07:17:18Z Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática Pérez Aguilar, Juan Alejandro Benítez Benítez, Ricardo Cannabis sativa cristalinidad azúcares reductores hidrólisis enzimática pretratamientos Cannabis sativa is a widely used plant in Colombia with medicinal purposes, for which its flower is used, generating high amounts of waste stems. In this study, Cannabis stems were pretreated with diluted sulfuric acid and sodium hydroxide and its effect was analyzed by infrared spectroscopy (FTIR-ATR). Remaining solids were submitted to enzymatic hydrolysis using commercial enzymatic mixtures: Celluclast 1.5 L and Cellic CTec3; production of reducing sugars was followed. Pretreatments removed hemicellulose and lignin, as evidence by the reduction in signals at 1734, 1540 and 1240 cm-1. Increase in cellulose crystallinity was also occasioned. Both pretreatments managed to increase reducing sugars production with the two enzymes used. Although, alkaline pretreatment was much more effective than acid pretreatment and allowed to produce up to 28.59 mg/mL. of reducing sugars with Celluclast 1.5 L and 24.94 mg/mL with Cellic CTec3. Mixing these two enzymes, using them sequentially, or increasing enzyme loading did not have and effect on the reached final concentration of reducing sugars. Cannabis sativa es una planta ampliamente utilizada en Colombia para propósitos medicinales, para lo que se usa su flor, generando altas cantidades de tallos residuales. En este estudio, tallos de Cannabis se pretrataron con ácido sulfúrico o hidróxido de sodio diluido y su efecto se analizó por espectroscopía de infrarrojo (FTIR-ATR). Los sólidos remanentes se llevaron a hidrólisis enzimática usando dos mezclas enzimáticas comerciales: Celluclast 1.5 L y Cellic CTec3; se hizo seguimiento a la producción de azúcares reductores. Los pretratamientos removieron hemicelulosa y lignina, evidenciado por la reducción en las señales en 1734, 1540 y 1240 cm-1. También ocasionaron un incremento en la cristalinidad de la celulosa. Ambos pretratamientos lograron aumentar la producción de azúcares en la hidrólisis con las dos enzimas trabajadas. Aunque el pretratamiento alcalino fue mucho más efectivo que el ácido y permitió producir hasta 28.59 mg/mL de azúcares con Celluclast 1.5 L y 24.94 mg/mL con Cellic CTec3. El mezclar estas enzimas, usarlas de forma secuencial, o incrementar la carga enzimática no tuvo un efecto sobre la concentración final de azúcares alcanzada. Universidad Pedagógica y Tecnológica de Colombia 2024-04-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15304 10.19053/01217488.v15.n1.2024.15304 Ciencia En Desarrollo; Vol. 15 No. 1 (2024): Vol 15, Núm.1 (2024): Enero-Junio Ciencia en Desarrollo; Vol. 15 Núm. 1 (2024): Vol 15, Núm.1 (2024): Enero-Junio 2462-7658 0121-7488 Español
spellingShingle Cannabis sativa
cristalinidad
azúcares reductores
hidrólisis enzimática
pretratamientos
Pérez Aguilar, Juan Alejandro
Benítez Benítez, Ricardo
Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática
title Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática
title_full Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática
title_fullStr Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática
title_full_unstemmed Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática
title_short Aprovechamiento de tallos de Cannabis sativa por pretratamiento termoquímico e hidrólisis enzimática
title_sort aprovechamiento de tallos de cannabis sativa por pretratamiento termoquimico e hidrolisis enzimatica
topic Cannabis sativa
cristalinidad
azúcares reductores
hidrólisis enzimática
pretratamientos
topic_facet Cannabis sativa
cristalinidad
azúcares reductores
hidrólisis enzimática
pretratamientos
url https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15304
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