Zebrafish (Danio Rerio) As An In Vivo Model For Genotoxicity Studies: Evaluation Of Numerical Chromosomal Instability
The agricultural, livestock and fish farming activities carried out in Colombia have contributed to the current contamination with pesticides of water sources, this being a constantly rising environmental problem. One such water source with a high rate of contamination is Lake Tota. This lake has be...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2024
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15555 |
| _version_ | 1801706358899212288 |
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| author | Rondón Lagos, Sandra Milena Rodríguez Sosa , Natalia Jaramillo García , Diego F Sánchez Espejo , Ángel Iván Salazar Salazar , Marleny |
| author_facet | Rondón Lagos, Sandra Milena Rodríguez Sosa , Natalia Jaramillo García , Diego F Sánchez Espejo , Ángel Iván Salazar Salazar , Marleny |
| author_sort | Rondón Lagos, Sandra Milena |
| collection | OJS |
| description | The agricultural, livestock and fish farming activities carried out in Colombia have contributed to the current contamination with pesticides of water sources, this being a constantly rising environmental problem. One such water source with a high rate of contamination is Lake Tota. This lake has been listed as one of the most threatened ecosystems on the planet by the global network of wetlands. Despite the environmental problems that contamination of water sources represents in our country, there are very few studies that investigate the cytogenetic damage generated by exposure to toxic agents. In this regard, an optimal biological model for the evaluation of the genotoxic effects of occupational or environmental exposure to pesticides is the zebrafish, due to its sensitivity to contaminants, sensitivity evidenced by damage to chromosomal material. Considering the above, the aim of this study was to standardize banding cytogenetics (GTG Banding) and molecular cytogenetics (Fluorescence In situ Hybridization-FISH) techniques, for their application in genotoxicity studies using zebrafish (Danio rerio) larvae as an in vivo model. The development of this study allowed the standardization of the GTG banding technique for the chromosome count in zebrafish larvae, as well as the standardization of the FISH technique, important in the evaluation of chromosomal instability. The standardization of banding and molecular cytogenetics on zebrafish, constitutes a very important tool for the application of in vivo study models that allow evaluate the chromosomal damage generates by the genotoxic agent’s exposure, including the pesticides. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-15555 |
| institution | Revista Ciencia en Desarrollo |
| language | spa |
| publishDate | 2024 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-155552024-05-11T07:17:13Z Zebrafish (Danio Rerio) As An In Vivo Model For Genotoxicity Studies: Evaluation Of Numerical Chromosomal Instability Pez Cebra (Danio Rerio) Como Modelo In Vivo Para Estudios De Genotoxicidad: Evaluación De Inestabilidad Cromosómica Numérica Rondón Lagos, Sandra Milena Rodríguez Sosa , Natalia Jaramillo García , Diego F Sánchez Espejo , Ángel Iván Salazar Salazar , Marleny Citogenética, FISH, genotoxicidad, modelos in vivo, pez cebra Cytogenetic, FISH, genotoxicity, in vivo models, Zebrafish The agricultural, livestock and fish farming activities carried out in Colombia have contributed to the current contamination with pesticides of water sources, this being a constantly rising environmental problem. One such water source with a high rate of contamination is Lake Tota. This lake has been listed as one of the most threatened ecosystems on the planet by the global network of wetlands. Despite the environmental problems that contamination of water sources represents in our country, there are very few studies that investigate the cytogenetic damage generated by exposure to toxic agents. In this regard, an optimal biological model for the evaluation of the genotoxic effects of occupational or environmental exposure to pesticides is the zebrafish, due to its sensitivity to contaminants, sensitivity evidenced by damage to chromosomal material. Considering the above, the aim of this study was to standardize banding cytogenetics (GTG Banding) and molecular cytogenetics (Fluorescence In situ Hybridization-FISH) techniques, for their application in genotoxicity studies using zebrafish (Danio rerio) larvae as an in vivo model. The development of this study allowed the standardization of the GTG banding technique for the chromosome count in zebrafish larvae, as well as the standardization of the FISH technique, important in the evaluation of chromosomal instability. The standardization of banding and molecular cytogenetics on zebrafish, constitutes a very important tool for the application of in vivo study models that allow evaluate the chromosomal damage generates by the genotoxic agent’s exposure, including the pesticides. Las actividades agrícolas, pecuarias y piscícolas que se desarrollan en Colombia han contribuido a la contaminación actual de las fuentes hídricas con plaguicidas, siendo este un problema ambiental en constante ascenso. Una de las fuentes hídricas con una alta tasa de contaminación es el lago Tota. Este lago ha sido catalogado como uno de los ecosistemas más amenazados del planeta por la red mundial de humedales. A pesar de los problemas ambientales que representa la contaminación de las fuentes hídricas en el país, existen muy pocos estudios que investiguen el daño citogenético generado por la exposición a agentes tóxicos. A este respecto, un modelo biológico óptimo para la evaluación de los efectos genotóxicos por la exposición ocupacional o ambiental a plaguicidas lo constituye el pez cebra, debido a su sensibilidad a los contaminantes, sensibilidad evidenciada por el daño al material cromosómico. Considerando lo anterior, el objetivo de esta investigación fue estandarizar técnicas de citogenética de bandas (Bandeo GTG) y de citogenética molecular (Hibridación In Situ por Fluorescencia - FISH), para su aplicación en estudios de genotoxicidad utilizando como modelo in vivo, larvas de pez cebra (Danio rerio). El desarrollo de este estudio permitió la estandarización de la técnica de Bandeo GTG para el conteo de cromosomas en larvas de pez cebra, así como la estandarización de la técnica FISH, importante en la evaluación de la inestabilidad cromosómica. La estandarización de técnicas de citogenética de bandas y de citogenética molecular en pez cebra, se constituye como una herramienta muy importante para la aplicación de modelos de estudio in vivo que permitan evaluar el daño cromosómico generado por la exposición a agentes genotóxicos, incluidos los plaguicidas. Universidad Pedagógica y Tecnológica de Colombia 2024-04-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15555 10.19053/01217488.v15.n1.2024.15555 Ciencia En Desarrollo; Vol. 15 No. 1 (2024): Vol 15, Núm.1 (2024): Enero-Junio Ciencia en Desarrollo; Vol. 15 Núm. 1 (2024): Vol 15, Núm.1 (2024): Enero-Junio 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15555/14058 |
| spellingShingle | Citogenética, FISH, genotoxicidad, modelos in vivo, pez cebra Cytogenetic, FISH, genotoxicity, in vivo models, Zebrafish Rondón Lagos, Sandra Milena Rodríguez Sosa , Natalia Jaramillo García , Diego F Sánchez Espejo , Ángel Iván Salazar Salazar , Marleny Zebrafish (Danio Rerio) As An In Vivo Model For Genotoxicity Studies: Evaluation Of Numerical Chromosomal Instability |
| title | Zebrafish (Danio Rerio) As An In Vivo Model For Genotoxicity Studies: Evaluation Of Numerical Chromosomal Instability |
| title_alt | Pez Cebra (Danio Rerio) Como Modelo In Vivo Para Estudios De Genotoxicidad: Evaluación De Inestabilidad Cromosómica Numérica |
| title_full | Zebrafish (Danio Rerio) As An In Vivo Model For Genotoxicity Studies: Evaluation Of Numerical Chromosomal Instability |
| title_fullStr | Zebrafish (Danio Rerio) As An In Vivo Model For Genotoxicity Studies: Evaluation Of Numerical Chromosomal Instability |
| title_full_unstemmed | Zebrafish (Danio Rerio) As An In Vivo Model For Genotoxicity Studies: Evaluation Of Numerical Chromosomal Instability |
| title_short | Zebrafish (Danio Rerio) As An In Vivo Model For Genotoxicity Studies: Evaluation Of Numerical Chromosomal Instability |
| title_sort | zebrafish danio rerio as an in vivo model for genotoxicity studies evaluation of numerical chromosomal instability |
| topic | Citogenética, FISH, genotoxicidad, modelos in vivo, pez cebra Cytogenetic, FISH, genotoxicity, in vivo models, Zebrafish |
| topic_facet | Citogenética, FISH, genotoxicidad, modelos in vivo, pez cebra Cytogenetic, FISH, genotoxicity, in vivo models, Zebrafish |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/15555 |
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