Biostimulant activity of humic acids derived from goat manure vermicompost and lignite in relation to their structure and interaction with a PGPR strain under semiarid conditions
The use of humified organic matter (HOM)-based plant biostimulants and plant growth-promoting rhizobacteria (PGPR) has emerged as a promising approach to enhance agricultural productivity in arid and semiarid environments. However, the bioactivity of humic stimulants varies based on their chemical c...
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| Format: | Online |
| Language: | eng |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2023
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/15956 |
| _version_ | 1801706309414813696 |
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| author | Valero, Nelson Ustates, Yeison Pantoja-Guerra, Manuel |
| author_facet | Valero, Nelson Ustates, Yeison Pantoja-Guerra, Manuel |
| author_sort | Valero, Nelson |
| collection | OJS |
| description | The use of humified organic matter (HOM)-based plant biostimulants and plant growth-promoting rhizobacteria (PGPR) has emerged as a promising approach to enhance agricultural productivity in arid and semiarid environments. However, the bioactivity of humic stimulants varies based on their chemical composition, and the synergistic effects of co-applying these biostimulants remain to be fully elucidated. In this research, we investigated the structural and bioactive characteristics of humic acids derived from goat manure vermicompost (HAVC) and lignite coal (HAC). Additionally, we explored the plant growth-promoting effects of each humic acid (HA) in conjunction with the Bacillus mycoides strain BSC25 (Bm) on corn plants in arid conditions. To assess the relationship between structure and bioactivity, we determined the supramolecular composition of the HAs and evaluated their effectiveness through a corn coleoptile elongation test. Subsequently, we conducted biostimulation tests on maize seedlings in a growth chamber and performed a field-based biostimulation test in a semi-arid region. Notably, HACs exhibited coleoptile elongation at lower concentrations (25-50 mg LC) compared to HAVCs, which required higher concentrations (100-200 mg LC) to achieve the same effect. These outcomes correlated with the supramolecular composition of HAs. The bioactivity of HACs was linked to their oxygen content, aromatic and carboxylic groups, whereas HAVCs' bioactivity was associated with their carbohydrate, aliphatic carbon, and hydrogen content. The application of both HAs, together with Bm, resulted in enhanced corn leaf biomass production in the growth chamber and under field conditions. This effect can be attributed to the hormone-like actions of HA and the PGPR activity of Bm. Interestingly, despite foliar application, HAs displayed bioactivity at the root level, as evidenced by increased root biomass in the field. These results indicate a PGPR effect of Bm that remained unaltered with co-application of HAVC. However, the joint application of Bm-HAC and Bm-HAVC reversed the positive effect of Bm on corn production under field conditions. This outcome likely relates to the hormone-like effects of HA and potential additive effects following Bm inoculation. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-15956 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | eng |
| publishDate | 2023 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-159562024-02-13T19:44:14Z Biostimulant activity of humic acids derived from goat manure vermicompost and lignite in relation to their structure and interaction with a PGPR strain under semiarid conditions Actividad bioestimulante de ácidos húmicos derivados de vermicompost de estiércol caprino y lignito en relación con su estructura e interacción con una cepa PGPR en condiciones semiáridas Valero, Nelson Ustates, Yeison Pantoja-Guerra, Manuel Bioactive compounds Zea mays Bacillus mycoides Dryland management Food insecurity Biotechnology Plant biostimulants Compuestos bioactivos Zea mays Bacillus mycoides Manejo de tierra secas Inseguridad alimentaria Biotecnología Bioestimulantes vegetales The use of humified organic matter (HOM)-based plant biostimulants and plant growth-promoting rhizobacteria (PGPR) has emerged as a promising approach to enhance agricultural productivity in arid and semiarid environments. However, the bioactivity of humic stimulants varies based on their chemical composition, and the synergistic effects of co-applying these biostimulants remain to be fully elucidated. In this research, we investigated the structural and bioactive characteristics of humic acids derived from goat manure vermicompost (HAVC) and lignite coal (HAC). Additionally, we explored the plant growth-promoting effects of each humic acid (HA) in conjunction with the Bacillus mycoides strain BSC25 (Bm) on corn plants in arid conditions. To assess the relationship between structure and bioactivity, we determined the supramolecular composition of the HAs and evaluated their effectiveness through a corn coleoptile elongation test. Subsequently, we conducted biostimulation tests on maize seedlings in a growth chamber and performed a field-based biostimulation test in a semi-arid region. Notably, HACs exhibited coleoptile elongation at lower concentrations (25-50 mg LC) compared to HAVCs, which required higher concentrations (100-200 mg LC) to achieve the same effect. These outcomes correlated with the supramolecular composition of HAs. The bioactivity of HACs was linked to their oxygen content, aromatic and carboxylic groups, whereas HAVCs' bioactivity was associated with their carbohydrate, aliphatic carbon, and hydrogen content. The application of both HAs, together with Bm, resulted in enhanced corn leaf biomass production in the growth chamber and under field conditions. This effect can be attributed to the hormone-like actions of HA and the PGPR activity of Bm. Interestingly, despite foliar application, HAs displayed bioactivity at the root level, as evidenced by increased root biomass in the field. These results indicate a PGPR effect of Bm that remained unaltered with co-application of HAVC. However, the joint application of Bm-HAC and Bm-HAVC reversed the positive effect of Bm on corn production under field conditions. This outcome likely relates to the hormone-like effects of HA and potential additive effects following Bm inoculation. El uso de bioestimulantes vegetales a base materia orgánica humificada (MOH) y PGPR’s es una tecnología en vía de consolidación para promover la productividad agrícola bajo condiciones marginales. Sin embargo, la bioactividad de los estimulantes húmicos varía de acuerdo con su naturaleza química y aun es necesario explicar los efectos sobre la aplicación conjunta de estos dos agentes bioestimulantes. En este trabajo se analizó la relación entre la estructura y bioactividad de ácidos húmicos obtenidos de lombricompost de estiércol caprino (HAVC) y de un carbón tipo lignito (HAC), así como el efecto promotor del crecimiento vegetal de cada uno de estos ácidos húmicos (AH) aplicados en conjunto de la cepa Bacillus mycoides BSC25 (Bm), sobre plantas de maíz, bajo condiciones de aridez. Para ello se determinó la composición supramolecular de los HA, su bioactividad fue evaluada mediante el test de elongación de coleóptilos de maíz, se desarrollaron ensayos de bioestimulación sobre plántulas de maíz en cámara de crecimiento, finalmente se desarrolló un ensayo de bioestimulación en maíz en condiciones de campo en una zona semiárida. Los HAC causaron elongación de coleóptilos a una concentración inferior (25-50 mg CL), en comparación con los HAVC, que causaron el mismo efecto a una concentración más alta (100-200 mg CL); se encontró una asociación entre estos resultados y la composición supramolecular de los HA. La bioactividad de los HAC estuvo asociada a su contenido de oxígeno, grupos aromáticos y carboxílicos; mientras que la bioactividad de los HAVC se correlacionó con su contenido de carbohidratos, carbono alifático y de hidrógeno. La aplicación de ambos HA, Bm y la aplicación conjunta HA- Bm, promovió la producción de biomasa foliar de maíz en cámara de crecimiento y en condiciones de campo. Esto se puede atribuir a los efectos hormone-like de los HA y a la actividad PGPR de Bm. Aunque los HA fueron aplicados por aspersión foliar, mostraron bioactividad a nivel radicular. Los resultados de biomasa radicular en campo indican un efecto PGPR de Bm, el cual no se vio modificado por la aplicación conjunta con HAVC. Sin embargo, la producción de mazorcas en condiciones de campo fue promovida por la actividad de Bm, pero este efecto fue revertido por la aplicación conjunta Bm-HAC y Bm-HAVC. Estos resultados están asociados a los efectos hormonales de los HA y de posibles efectos aditivos tras la inoculación con Bm. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2023-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/15956 10.17584/rcch.2023v17i2.15956 Revista Colombiana de Ciencias Hortícolas; Vol. 17 No. 2 (2023); e15956 Revista Colombiana de Ciencias Hortícolas; Vol. 17 Núm. 2 (2023); e15956 Revista Colombiana de Ciencias Hortícolas; Vol. 17 No 2 (2023); e15956 Revista Colombiana de Ciencias Hortícolas; V. 17 N. 2 (2023); e15956 Revista Colombiana de Ciencias Hortícolas; v. 17 n. 2 (2023); e15956 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/15956/13409 Colombia; La Guajira; Riohacha Colombia; La Guajira; Riohacha Copyright (c) 2023 Revista Colombiana de Ciencias Hortícolas http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Bioactive compounds Zea mays Bacillus mycoides Dryland management Food insecurity Biotechnology Plant biostimulants Compuestos bioactivos Zea mays Bacillus mycoides Manejo de tierra secas Inseguridad alimentaria Biotecnología Bioestimulantes vegetales Valero, Nelson Ustates, Yeison Pantoja-Guerra, Manuel Biostimulant activity of humic acids derived from goat manure vermicompost and lignite in relation to their structure and interaction with a PGPR strain under semiarid conditions |
| title | Biostimulant activity of humic acids derived from goat manure vermicompost and lignite in relation to their structure and interaction with a PGPR strain under semiarid conditions |
| title_alt | Actividad bioestimulante de ácidos húmicos derivados de vermicompost de estiércol caprino y lignito en relación con su estructura e interacción con una cepa PGPR en condiciones semiáridas |
| title_full | Biostimulant activity of humic acids derived from goat manure vermicompost and lignite in relation to their structure and interaction with a PGPR strain under semiarid conditions |
| title_fullStr | Biostimulant activity of humic acids derived from goat manure vermicompost and lignite in relation to their structure and interaction with a PGPR strain under semiarid conditions |
| title_full_unstemmed | Biostimulant activity of humic acids derived from goat manure vermicompost and lignite in relation to their structure and interaction with a PGPR strain under semiarid conditions |
| title_short | Biostimulant activity of humic acids derived from goat manure vermicompost and lignite in relation to their structure and interaction with a PGPR strain under semiarid conditions |
| title_sort | biostimulant activity of humic acids derived from goat manure vermicompost and lignite in relation to their structure and interaction with a pgpr strain under semiarid conditions |
| topic | Bioactive compounds Zea mays Bacillus mycoides Dryland management Food insecurity Biotechnology Plant biostimulants Compuestos bioactivos Zea mays Bacillus mycoides Manejo de tierra secas Inseguridad alimentaria Biotecnología Bioestimulantes vegetales |
| topic_facet | Bioactive compounds Zea mays Bacillus mycoides Dryland management Food insecurity Biotechnology Plant biostimulants Compuestos bioactivos Zea mays Bacillus mycoides Manejo de tierra secas Inseguridad alimentaria Biotecnología Bioestimulantes vegetales |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/15956 |
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