Propagation of Cannabis sativa (L.) plants through cuttings and use of auxin phytoregulators

Cannabis sativa (L.) is an annual herbaceous species which has been extensively cultivated and used as a medicinal plant and for recreational purposes. Its medicinal value is primarily attributed to a group of secondary metabolites called phytocannabinoids, which are mainly concentrated in the essen...

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Main Authors: Porras-García, Beatriz, Pinzón-Sandoval, Elberth Hernado, Almanza-Merchán, Pedro José
Format: Online
Language:eng
Published: Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2023
Subjects:
Online Access:https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16428
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author Porras-García, Beatriz
Pinzón-Sandoval, Elberth Hernado
Almanza-Merchán, Pedro José
author_facet Porras-García, Beatriz
Pinzón-Sandoval, Elberth Hernado
Almanza-Merchán, Pedro José
author_sort Porras-García, Beatriz
collection OJS
description Cannabis sativa (L.) is an annual herbaceous species which has been extensively cultivated and used as a medicinal plant and for recreational purposes. Its medicinal value is primarily attributed to a group of secondary metabolites called phytocannabinoids, which are mainly concentrated in the essential oils of unfertilized female flowers. Asexual reproduction allows for the offspring of plant material while maintaining the genetic characteristics of the mother plant. The aim of the research was to improve the vegetative propagation process of Cannabis sativa (L.) cv. Eco-2, which has characteristics of medicinal phytocannabinoid producers, using auxin plant growth regulators (NAA and IBA) at different doses. The study was conducted under greenhouse conditions in the municipality of Pesca (Colombia). Doses of 400, 800 and 1,000 mg L-1 and a control without application were evaluated for plant height, stem diameter, number of branches, root volume and length, and rooting percentage. The best treatment was obtained with the application of NAA at a dose of 800 mg L-1, which resulted in a rooting percentage of 86.6%, a root length and volume of 9.0 cm and 5.0 cm3, and an increase in the length of the cutting by 3.8 cm, with significant differences compared to the treatment without application, after 4 weeks of planting. The use of auxin plant growth regulators becomes an alternative with high potential for the propagation of medicinal cannabinoid-producing plants.
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spelling oai:oai.revistas.uptc.edu.co:article-164282024-02-14T21:28:32Z Propagation of Cannabis sativa (L.) plants through cuttings and use of auxin phytoregulators Propagación de plantas de cannabis sativa (L.) mediante esquejes y uso de fitorreguladores auxínicos Porras-García, Beatriz Pinzón-Sandoval, Elberth Hernado Almanza-Merchán, Pedro José Phytohormones Phytocannabinoids Vegetative reproduction Physiological regulator Hemp Plant propagation Hemp Fitohormonas Fitocannabinoides Reproducción vegetativa Regulador fisiológico Cáñamo Propagación de plantas Cáñamo Cannabis sativa (L.) is an annual herbaceous species which has been extensively cultivated and used as a medicinal plant and for recreational purposes. Its medicinal value is primarily attributed to a group of secondary metabolites called phytocannabinoids, which are mainly concentrated in the essential oils of unfertilized female flowers. Asexual reproduction allows for the offspring of plant material while maintaining the genetic characteristics of the mother plant. The aim of the research was to improve the vegetative propagation process of Cannabis sativa (L.) cv. Eco-2, which has characteristics of medicinal phytocannabinoid producers, using auxin plant growth regulators (NAA and IBA) at different doses. The study was conducted under greenhouse conditions in the municipality of Pesca (Colombia). Doses of 400, 800 and 1,000 mg L-1 and a control without application were evaluated for plant height, stem diameter, number of branches, root volume and length, and rooting percentage. The best treatment was obtained with the application of NAA at a dose of 800 mg L-1, which resulted in a rooting percentage of 86.6%, a root length and volume of 9.0 cm and 5.0 cm3, and an increase in the length of the cutting by 3.8 cm, with significant differences compared to the treatment without application, after 4 weeks of planting. The use of auxin plant growth regulators becomes an alternative with high potential for the propagation of medicinal cannabinoid-producing plants. Cannabis sativa (L.) es una especie herbácea anual que ha sido ampliamente cultivada y utilizada como planta medicinal y con fines recreativos. Su valor medicinal se atribuye principalmente a un grupo de metabolitos secundarios llamados fitocannabinoides, que se concentran principalmente en los aceites esenciales de flores femeninas no fertilizadas. La reproducción asexual permite la descendencia de material vegetal, manteniendo las características genéticas de la planta madre. El objetivo de la investigación fue el de mejorar el proceso de propagación vegetativa de esquejes de Cannabis sativa (L.) cv. Eco-2 con características de productoras de fitocannabinoides de uso medicinal, mediante el empleo de fitorreguladores auxínicos de enraizamiento (ANA y AIB) en diferentes dosis. El estudio se realizó bajo condiciones de invernadero en el municipio de Pesca (Colombia). Se evaluaron dosis de 400, 800 y 1.000 mg L-1 y un testigo sin aplicación, se determinó la altura de la planta, el diámetro del tallo, número de ramas, volumen y longitud de raíz, y el porcentaje de enraizamiento. El mejor tratamiento se obtuvo con la aplicación de ANA en una dosis de 800 mg L-1 presentó un porcentaje de enraizamiento del 86,6%, una longitud y un volumen de raíz de 9,0 cm y 5,0 cm3, y un aumento de la longitud del esqueje de 3,8 cm, con diferencias significativas frente al tratamiento sin aplicación, luego de 4 semanas después de la siembra. El uso de fitorreguladores auxínicos se convierte en una alternativa con alto potencial para la propagación de plantas productoras de cannabinoides para uso medicinal. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2023-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16428 10.17584/rcch.2023v17i3.16428 Revista Colombiana de Ciencias Hortícolas; Vol. 17 No. 3 (2023); e16428 Revista Colombiana de Ciencias Hortícolas; Vol. 17 Núm. 3 (2023); e16428 Revista Colombiana de Ciencias Hortícolas; Vol. 17 No 3 (2023); e16428 Revista Colombiana de Ciencias Hortícolas; V. 17 N. 3 (2023); e16428 Revista Colombiana de Ciencias Hortícolas; v. 17 n. 3 (2023); e16428 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16428/13522 Colombia; Boyaca; Pesca Colombia; Boyacá; Pesca Copyright (c) 2023 Revista Colombiana de Ciencias Hortícolas http://creativecommons.org/licenses/by-nc-sa/4.0
spellingShingle Phytohormones
Phytocannabinoids
Vegetative reproduction
Physiological regulator
Hemp
Plant propagation
Hemp
Fitohormonas
Fitocannabinoides
Reproducción vegetativa
Regulador fisiológico
Cáñamo
Propagación de plantas
Cáñamo
Porras-García, Beatriz
Pinzón-Sandoval, Elberth Hernado
Almanza-Merchán, Pedro José
Propagation of Cannabis sativa (L.) plants through cuttings and use of auxin phytoregulators
title Propagation of Cannabis sativa (L.) plants through cuttings and use of auxin phytoregulators
title_alt Propagación de plantas de cannabis sativa (L.) mediante esquejes y uso de fitorreguladores auxínicos
title_full Propagation of Cannabis sativa (L.) plants through cuttings and use of auxin phytoregulators
title_fullStr Propagation of Cannabis sativa (L.) plants through cuttings and use of auxin phytoregulators
title_full_unstemmed Propagation of Cannabis sativa (L.) plants through cuttings and use of auxin phytoregulators
title_short Propagation of Cannabis sativa (L.) plants through cuttings and use of auxin phytoregulators
title_sort propagation of cannabis sativa l plants through cuttings and use of auxin phytoregulators
topic Phytohormones
Phytocannabinoids
Vegetative reproduction
Physiological regulator
Hemp
Plant propagation
Hemp
Fitohormonas
Fitocannabinoides
Reproducción vegetativa
Regulador fisiológico
Cáñamo
Propagación de plantas
Cáñamo
topic_facet Phytohormones
Phytocannabinoids
Vegetative reproduction
Physiological regulator
Hemp
Plant propagation
Hemp
Fitohormonas
Fitocannabinoides
Reproducción vegetativa
Regulador fisiológico
Cáñamo
Propagación de plantas
Cáñamo
url https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16428
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