Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens
The most important pathogens causing high damage to fruit and vegetable crops are bacteria and fungi; among them Xanthomonas and Fusarium respectively are in the phytopathogens responsible of diseases in those crops. In Colombia, the crop of Passiflora edulis f. edulis occupies a position preferenti...
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| Format: | Online |
| Language: | eng |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2023
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16474 |
| _version_ | 1801706311751041024 |
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| author | Núñez-Rangel, Vitelbina Rey-Suárez, Paola Serna, Alejandro Gómez-Robles, Jeisson Rey-Torres, Armando Morales, Estefanía |
| author_facet | Núñez-Rangel, Vitelbina Rey-Suárez, Paola Serna, Alejandro Gómez-Robles, Jeisson Rey-Torres, Armando Morales, Estefanía |
| author_sort | Núñez-Rangel, Vitelbina |
| collection | OJS |
| description | The most important pathogens causing high damage to fruit and vegetable crops are bacteria and fungi; among them Xanthomonas and Fusarium respectively are in the phytopathogens responsible of diseases in those crops. In Colombia, the crop of Passiflora edulis f. edulis occupies a position preferential within international market. However different pathologies affect their production, one of them is the infectious diseases and are caused by microorganisms as Xanthomonas and Fusarium. The control includes application of pesticides or antibiotics, but to some of them the microorganisms have develop resistance is widespread, and others are restricted associated to the impact on the environment and effects in animal and human healthy. Then find other alternatives is necessary, in this work the snake venoms of the vipers Bothrops asper and Porthidium nasutum were evaluated in ability to inhibit growth of these microorganism isolated of purple passion fruit crops with typical symptoms of diseases associated with infections by microorganisms. For these antimicrobial assays were performed in agar culture and broth microdilution. The results showed growth inhibition of 80% to B. asper against Xanthomonas axonopodis and 70% Xanthomonas perforans. The minimum inhibitory concentration was 31,2 µg mL-1 to B. asper and 500 µg mL-1 to P. nasutum to both bacteria. Nevertheless, the activity against F. oxysporum was low. The isolation of compounds responsible of this activity will contribute to the development of new molecules for antimicrobial agents against phytopathogens affecting purple passion fruit crops. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-16474 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | eng |
| publishDate | 2023 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-164742024-02-14T21:28:32Z Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens Actividad antimicrobiana del veneno de Bothrops asper y Porthidium nasutum sobre fitopatógenos de la maracuyá morada (Passiflora edulis f. edulis) Núñez-Rangel, Vitelbina Rey-Suárez, Paola Serna, Alejandro Gómez-Robles, Jeisson Rey-Torres, Armando Morales, Estefanía Snake venoms Plant infectious diseases Xanthomones Fusarium Bioprospecting Tropical fruits Venenos de serpientes Enfermedades infecciosas de las plantas Xantomonas Fusarium Bioprospección Frutas tropicales The most important pathogens causing high damage to fruit and vegetable crops are bacteria and fungi; among them Xanthomonas and Fusarium respectively are in the phytopathogens responsible of diseases in those crops. In Colombia, the crop of Passiflora edulis f. edulis occupies a position preferential within international market. However different pathologies affect their production, one of them is the infectious diseases and are caused by microorganisms as Xanthomonas and Fusarium. The control includes application of pesticides or antibiotics, but to some of them the microorganisms have develop resistance is widespread, and others are restricted associated to the impact on the environment and effects in animal and human healthy. Then find other alternatives is necessary, in this work the snake venoms of the vipers Bothrops asper and Porthidium nasutum were evaluated in ability to inhibit growth of these microorganism isolated of purple passion fruit crops with typical symptoms of diseases associated with infections by microorganisms. For these antimicrobial assays were performed in agar culture and broth microdilution. The results showed growth inhibition of 80% to B. asper against Xanthomonas axonopodis and 70% Xanthomonas perforans. The minimum inhibitory concentration was 31,2 µg mL-1 to B. asper and 500 µg mL-1 to P. nasutum to both bacteria. Nevertheless, the activity against F. oxysporum was low. The isolation of compounds responsible of this activity will contribute to the development of new molecules for antimicrobial agents against phytopathogens affecting purple passion fruit crops. Los patógenos más importantes que causan grandes daños a los cultivos de frutas y hortalizas son las bacterias y hongos; entre ellos se encuentran Xanthomonas y Fusarium, respectivamente. Estos fitopatógenos son responsables de varias enfermedades en los cultivos. En Colombia, el cultivo de Passiflora edulis f. edulis ocupa una posición preferencial dentro del mercado internacional. Sin embargo, diferentes patologías afectan su producción, una de ellas son las enfermedades infecciosas y son causadas precisamente por microorganismos como Xanthomonas y Fusarium. Su control incluye la aplicación de pesticidas o antibióticos, pero los microorganismos han desarrollado resistencia a algunos de ellos y otros están restringidos debido al impacto en el medio ambiente y por sus efectos en la salud animal y humana. Por ello, es necesario encontrar otras alternativas. Así, en este trabajo se evaluó la capacidad de los venenos de las víboras Bothrops asper y Porthidium nasutum para inhibir el crecimiento de microorganismos aislados de maracuyá morada. Para ello, se realizaron ensayos antimicrobianos en cultivos en agar y microdilución en caldo. Los resultados mostraron una inhibición del crecimiento del 80% de B. asper frente a Xanthomonas axonopodis y del 70% de Xanthomonas perforans. La concentración mínima de inhibición fue de 31,2 µg mL-1 para B. asper y de 500 µg mL-1 para P. nasutum para ambas bacterias. Sin embargo, la actividad contra F. oxysporum fue baja. El aislamiento del compuesto responsable de esta actividad contribuirá al desarrollo de nuevas moléculas de agentes antimicrobianos contra fitopatógenos que afectan los cultivos de maracuyá morada. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2023-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16474 10.17584/rcch.2023v17i3.16474 Revista Colombiana de Ciencias Hortícolas; Vol. 17 No. 3 (2023); e16474 Revista Colombiana de Ciencias Hortícolas; Vol. 17 Núm. 3 (2023); e16474 Revista Colombiana de Ciencias Hortícolas; Vol. 17 No 3 (2023); e16474 Revista Colombiana de Ciencias Hortícolas; V. 17 N. 3 (2023); e16474 Revista Colombiana de Ciencias Hortícolas; v. 17 n. 3 (2023); e16474 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16474/13690 Colombia; Antioquia Colombia; Antioquia Copyright (c) 2023 Revista Colombiana de Ciencias Hortícolas http://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Snake venoms Plant infectious diseases Xanthomones Fusarium Bioprospecting Tropical fruits Venenos de serpientes Enfermedades infecciosas de las plantas Xantomonas Fusarium Bioprospección Frutas tropicales Núñez-Rangel, Vitelbina Rey-Suárez, Paola Serna, Alejandro Gómez-Robles, Jeisson Rey-Torres, Armando Morales, Estefanía Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens |
| title | Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens |
| title_alt | Actividad antimicrobiana del veneno de Bothrops asper y Porthidium nasutum sobre fitopatógenos de la maracuyá morada (Passiflora edulis f. edulis) |
| title_full | Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens |
| title_fullStr | Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens |
| title_full_unstemmed | Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens |
| title_short | Antimicrobial activity of Bothrops asper and Porthidium nasutum venom on purple passion fruit (Passiflora edulis f. edulis) phytopathogens |
| title_sort | antimicrobial activity of bothrops asper and porthidium nasutum venom on purple passion fruit passiflora edulis f edulis phytopathogens |
| topic | Snake venoms Plant infectious diseases Xanthomones Fusarium Bioprospecting Tropical fruits Venenos de serpientes Enfermedades infecciosas de las plantas Xantomonas Fusarium Bioprospección Frutas tropicales |
| topic_facet | Snake venoms Plant infectious diseases Xanthomones Fusarium Bioprospecting Tropical fruits Venenos de serpientes Enfermedades infecciosas de las plantas Xantomonas Fusarium Bioprospección Frutas tropicales |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16474 |
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