Antifungal activity of Peperomia subspathulata Yunck against three phytopathogenic fungi: Aspergillus, Botrytis, and Penicillium species

Peperomia subspathulata Yunck is an aromatic plant of the Piperaceae family. This species is native to Colombia and Ecuador. It is used in food preparation for its aroma and has been traditionally used to treat blows and wounds. The present study evaluated the essential oil and the ethanolic extract...

Full description

Saved in:

Bibliographic Details
Main Author:	Gutiérrez, Doris
Format:	Online
Language:	eng
Published:	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2024
Subjects:	Phytopathogenic fungi Essential oil Ethanolic extract Medicinal plants Bio-assays Peperomia subspathulata Antifungal activity Aspergillus Botrytis Penicillium Hongos fitopatógenos Aceite esencial Extracto etanólico Plantas medicinales Bioensayos Actividad antifúngica
Online Access:	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16885
Tags:	Add Tag No Tags, Be the first to tag this record!

_version_	1801706313310273536
author	Gutiérrez, Doris
author_facet	Gutiérrez, Doris
author_sort	Gutiérrez, Doris
collection	OJS
description	Peperomia subspathulata Yunck is an aromatic plant of the Piperaceae family. This species is native to Colombia and Ecuador. It is used in food preparation for its aroma and has been traditionally used to treat blows and wounds. The present study evaluated the essential oil and the ethanolic extract of aerial parts from P. subspathulata against Aspergillus sp., Botrytis sp., and Penicillium sp. The essential oil was obtained by hydrodistillation, and gas chromatography coupled with mass spectrometry analyzed its composition. The ethanolic extract was obtained by maceration with ethanol 96%. The antifungal activity tests were conducted in a potato dextrose agar medium which different concentration of essential oil and extract were added. The main components identified in the essential oil were safrole (44.3%), α-bisabolol (24.2%), myristicin (4.7%), trans-β-caryophyllene (3.0%), viridiflorene (30%), α-humulene (2.3%), trans-nerolidol (1.5%), linalool (1.1%), methyleugenol (1.1%) and cis-farnesene (1.0%). The total phenolic content of the ethanolic extract was determined by the Folin-Ciocalteu method (48.5±0.5 mg gallic acid equivalent GAE/g of extract). The ethanolic extract and the essential oil inhibited the growth of microorganisms. The essential oil was the most effective against Botrytis sp. (MIC=500 µg mL-1) and Aspergillus (MIC=1,000 µg mL-1).
format	Online
id	oai:oai.revistas.uptc.edu.co:article-16885
institution	Revista Colombiana de Ciencias Hortícolas
language	eng
publishDate	2024
publisher	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
record_format	ojs
spelling	oai:oai.revistas.uptc.edu.co:article-168852024-06-11T12:49:18Z Antifungal activity of Peperomia subspathulata Yunck against three phytopathogenic fungi: Aspergillus, Botrytis, and Penicillium species Actividad antifúngica de Peperomia subspathulata Yunck frente a tres hongos fitopatógenos: Aspergillus, Botrytis y Penicillium Gutiérrez, Doris Phytopathogenic fungi Essential oil Ethanolic extract Medicinal plants Bio-assays Peperomia subspathulata Antifungal activity Aspergillus Botrytis Penicillium Hongos fitopatógenos Aceite esencial Extracto etanólico Plantas medicinales Bioensayos Peperomia subspathulata Actividad antifúngica Aspergillus Botrytis Penicillium Peperomia subspathulata Yunck is an aromatic plant of the Piperaceae family. This species is native to Colombia and Ecuador. It is used in food preparation for its aroma and has been traditionally used to treat blows and wounds. The present study evaluated the essential oil and the ethanolic extract of aerial parts from P. subspathulata against Aspergillus sp., Botrytis sp., and Penicillium sp. The essential oil was obtained by hydrodistillation, and gas chromatography coupled with mass spectrometry analyzed its composition. The ethanolic extract was obtained by maceration with ethanol 96%. The antifungal activity tests were conducted in a potato dextrose agar medium which different concentration of essential oil and extract were added. The main components identified in the essential oil were safrole (44.3%), α-bisabolol (24.2%), myristicin (4.7%), trans-β-caryophyllene (3.0%), viridiflorene (30%), α-humulene (2.3%), trans-nerolidol (1.5%), linalool (1.1%), methyleugenol (1.1%) and cis-farnesene (1.0%). The total phenolic content of the ethanolic extract was determined by the Folin-Ciocalteu method (48.5±0.5 mg gallic acid equivalent GAE/g of extract). The ethanolic extract and the essential oil inhibited the growth of microorganisms. The essential oil was the most effective against Botrytis sp. (MIC=500 µg mL-1) and Aspergillus (MIC=1,000 µg mL-1). Peperomia subspathulata Yunck es una planta aromática de la familia Piperaceae. Es una especie nativa de Colombia y Ecuador. Se usa en la preparación de alimentos por su aroma y ha sido usada tradicionalmente para tratar golpes y heridas. En el presente estudio se evaluó el aceite esencial y el extracto etanólico de las partes aéreas de P. subspathulata frente a Aspergillus sp., Botrytis sp. y Penicillium sp. El aceite esencial fue obtenido por hidrodestilación y su composición fue analizada por cromatografía de gases acoplada a espectrometría de masas. El extracto etanólico se obtuvo por maceración con etanol al 96%. Los ensayos de actividad antifúngica fueron realizados en un medio de agar dextrosa papa al cual se le adicionaron diferentes concentraciones del aceite esencial o extracto. Los compuestos mayoritarios identificados en el aceite esencial fueron safrol (44,3%), α-bisabolol (24,2%), miristicina (4,7%), trans-β-cariofileno (3,0%), viridifloreno (3,0%), α-humuleno (2,3%), trans-nerolidol (1.5%), linalol (1,1%), metileugenol (1,1%) y cis-farneseno (1,0%). El contenido de fenoles totales del extracto etanólico se determinó por el método de Folin-Ciocalteu (48,5±0,5 mg EAG/g de extracto). El extracto etanólico y el aceite esencial inhibieron el crecimiento de los microorganismos. El aceite esencial fue el más efectivo frente a Botrytis sp. (CMI=500 µg mL-1) y Aspergillus (CMI=1.000 µg mL-1). Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2024-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16885 10.17584/rcch.2024v18i1.16885 Revista Colombiana de Ciencias Hortícolas; Vol. 18 No. 1 (2024); e16885 Revista Colombiana de Ciencias Hortícolas; Vol. 18 Núm. 1 (2024); e16885 Revista Colombiana de Ciencias Hortícolas; Vol. 18 No 1 (2024); e16885 Revista Colombiana de Ciencias Hortícolas; V. 18 N. 1 (2024); e16885 Revista Colombiana de Ciencias Hortícolas; v. 18 n. 1 (2024); e16885 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16885/14013 Colombia, Bogota, DC Colombia, Bogotá, DC Copyright (c) 2024 Revista Colombiana de Ciencias Hortícolas http://creativecommons.org/licenses/by-nc-sa/4.0
spellingShingle	Phytopathogenic fungi Essential oil Ethanolic extract Medicinal plants Bio-assays Peperomia subspathulata Antifungal activity Aspergillus Botrytis Penicillium Hongos fitopatógenos Aceite esencial Extracto etanólico Plantas medicinales Bioensayos Peperomia subspathulata Actividad antifúngica Aspergillus Botrytis Penicillium Gutiérrez, Doris Antifungal activity of Peperomia subspathulata Yunck against three phytopathogenic fungi: Aspergillus, Botrytis, and Penicillium species
title	Antifungal activity of Peperomia subspathulata Yunck against three phytopathogenic fungi: Aspergillus, Botrytis, and Penicillium species
title_alt	Actividad antifúngica de Peperomia subspathulata Yunck frente a tres hongos fitopatógenos: Aspergillus, Botrytis y Penicillium
title_full	Antifungal activity of Peperomia subspathulata Yunck against three phytopathogenic fungi: Aspergillus, Botrytis, and Penicillium species
title_fullStr	Antifungal activity of Peperomia subspathulata Yunck against three phytopathogenic fungi: Aspergillus, Botrytis, and Penicillium species
title_full_unstemmed	Antifungal activity of Peperomia subspathulata Yunck against three phytopathogenic fungi: Aspergillus, Botrytis, and Penicillium species
title_short	Antifungal activity of Peperomia subspathulata Yunck against three phytopathogenic fungi: Aspergillus, Botrytis, and Penicillium species
title_sort	antifungal activity of peperomia subspathulata yunck against three phytopathogenic fungi aspergillus botrytis and penicillium species
topic	Phytopathogenic fungi Essential oil Ethanolic extract Medicinal plants Bio-assays Peperomia subspathulata Antifungal activity Aspergillus Botrytis Penicillium Hongos fitopatógenos Aceite esencial Extracto etanólico Plantas medicinales Bioensayos Peperomia subspathulata Actividad antifúngica Aspergillus Botrytis Penicillium
topic_facet	Phytopathogenic fungi Essential oil Ethanolic extract Medicinal plants Bio-assays Peperomia subspathulata Antifungal activity Aspergillus Botrytis Penicillium Hongos fitopatógenos Aceite esencial Extracto etanólico Plantas medicinales Bioensayos Peperomia subspathulata Actividad antifúngica Aspergillus Botrytis Penicillium
url	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/16885
work_keys_str_mv	AT gutierrezdoris antifungalactivityofpeperomiasubspathulatayunckagainstthreephytopathogenicfungiaspergillusbotrytisandpenicilliumspecies AT gutierrezdoris actividadantifungicadepeperomiasubspathulatayunckfrenteatreshongosfitopatogenosaspergillusbotrytisypenicillium