Microbial Consortiums with Promissory Lactic Acid Activity, Isolated from Native Bacterial Inoculants for Silages
This work aim was to isolate and characterize strains of LAB, from native microbial preparations (PM), using agroindustrial wastes as whey, bovine manure and rumen contents, designed as biological inoculants for silage processes of post harvest organic waste. Five treatments (microbial preparations,...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2014
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/3484 |
| _version_ | 1801706462468112384 |
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| author | Díaz-Monroy, Byron Leoncio Iglesias, Arabel Elías Valiño-Cabrera, Elaine |
| author_facet | Díaz-Monroy, Byron Leoncio Iglesias, Arabel Elías Valiño-Cabrera, Elaine |
| author_sort | Díaz-Monroy, Byron Leoncio |
| collection | OJS |
| description | This work aim was to isolate and characterize strains of LAB, from native microbial preparations (PM), using agroindustrial wastes as whey, bovine manure and rumen contents, designed as biological inoculants for silage processes of post harvest organic waste. Five treatments (microbial preparations, PM + 33% inoculant residue) with 3 replications were evaluated: 1) Treatment control without inoculum (PMSI), 2) Whey (PMSL), 3) Cattle manure (PMEB), 4) Combination of Serum + manure (PMM), 5) Ruminal contents (PMCR). Also were added in all: 20% molasses, 1% urea, mineral salt 1%, and water 45%. They were fermented in plastic containers covered for 96 hours, at 14 °C. hence 3 samples of 500 mL from each PM were obtained and planted in agar MRS (Man, Rogosa and Sharpe), incubated at 37 °C for 72 h under anaerobic conditions (AnaeroGen, Oxoid). LAB strains are catalase and oxidase negative, coco, coco-bacilli and gram-positive non-spore forming. The biochemical identification was performed with the API-50 CHL (BioMérieux, France) system and the database APILAB Plus version 3.3.3. 39 strains LAB were isolated, 4 from PMSI (2 Lactobacillus plantarum, 1 L. brevis, 1 Lactococcus lactis), 12 from PMSL (3 Lactobacillus rhamnosus, 3 L. casei, 2 L. acidophilus and 2 L. paracasei and 2 L. fermentum), 7 from PMEB (3 Streptococcus bovis, 2 Lactobacillus plantarum and 2 Leuconostoc mesenteroides), 10 from PMM (3 Lactobacillus casei, 2 L. plantarum, 2 L. acidophilus, 1 L. fermentum, 1 L. rhamnosus and 1 L. paracasei) and 6 from PMCR (2 Lactobacillus vitulinus, 2 L. ruminus, 1 L. reuteri and 1 Pediococcus damnosus). As conclusion the best source of LAB strains was PMSL, which has the advantage to be a possible bacterial silage inoculant. In all PM were identified homofermentative and heterofermentative LAB strains, associated in microbial consortia. Aditionnally a technological alternative was created to profit from three new agro-industrial wastes. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-3484 |
| institution | Revista Ciencia y Agricultura |
| language | spa |
| publishDate | 2014 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-34842018-11-21T00:16:45Z Microbial Consortiums with Promissory Lactic Acid Activity, Isolated from Native Bacterial Inoculants for Silages Consorcios microbianos con actividad ácido-láctica promisoria aislados desde inoculantes bacterianos nativos para ensilajes Díaz-Monroy, Byron Leoncio Iglesias, Arabel Elías Valiño-Cabrera, Elaine lactic acid bacteria LAB microbial preparation MP whey bovine manure ruminal content Bacterias ácido lácticas BAL Preparado microbiano PM Suero de leche Estiércol bovino Contenido ruminal. This work aim was to isolate and characterize strains of LAB, from native microbial preparations (PM), using agroindustrial wastes as whey, bovine manure and rumen contents, designed as biological inoculants for silage processes of post harvest organic waste. Five treatments (microbial preparations, PM + 33% inoculant residue) with 3 replications were evaluated: 1) Treatment control without inoculum (PMSI), 2) Whey (PMSL), 3) Cattle manure (PMEB), 4) Combination of Serum + manure (PMM), 5) Ruminal contents (PMCR). Also were added in all: 20% molasses, 1% urea, mineral salt 1%, and water 45%. They were fermented in plastic containers covered for 96 hours, at 14 °C. hence 3 samples of 500 mL from each PM were obtained and planted in agar MRS (Man, Rogosa and Sharpe), incubated at 37 °C for 72 h under anaerobic conditions (AnaeroGen, Oxoid). LAB strains are catalase and oxidase negative, coco, coco-bacilli and gram-positive non-spore forming. The biochemical identification was performed with the API-50 CHL (BioMérieux, France) system and the database APILAB Plus version 3.3.3. 39 strains LAB were isolated, 4 from PMSI (2 Lactobacillus plantarum, 1 L. brevis, 1 Lactococcus lactis), 12 from PMSL (3 Lactobacillus rhamnosus, 3 L. casei, 2 L. acidophilus and 2 L. paracasei and 2 L. fermentum), 7 from PMEB (3 Streptococcus bovis, 2 Lactobacillus plantarum and 2 Leuconostoc mesenteroides), 10 from PMM (3 Lactobacillus casei, 2 L. plantarum, 2 L. acidophilus, 1 L. fermentum, 1 L. rhamnosus and 1 L. paracasei) and 6 from PMCR (2 Lactobacillus vitulinus, 2 L. ruminus, 1 L. reuteri and 1 Pediococcus damnosus). As conclusion the best source of LAB strains was PMSL, which has the advantage to be a possible bacterial silage inoculant. In all PM were identified homofermentative and heterofermentative LAB strains, associated in microbial consortia. Aditionnally a technological alternative was created to profit from three new agro-industrial wastes. El objetivo de este trabajo fue aislar y caracterizar cepas de bacterias ácido lácticas (BAL) a partir de preparados microbianos (PM) nativos elaborados a base de residuos agroindustriales, como suero de leche, estiércol bovino y contenido ruminal, y diseñados como inoculantes biológicos para procesos de ensilaje de residuales orgánicos poscosecha. Se evaluaron 5 tratamientos (preparados microbianos, PM + 33% de residuo inoculante), con 3 réplicas: 1) Tratamiento control sin inoculante (PMSI), 2) Suero de leche (PMSL), 3) Estiércol bovino (PMEB), 4) Combinación de suero+estiércol (PMM) y 5) Contenido ruminal (PMCR), además, en todos se adicionó: melaza (20%), urea (1%), sal mineral (1%) y agua (45%). En recipientes plásticos se fermentaron tapados durante 96 horas, a 14 °C, de allí se obtuvieron 3 muestras de 500 mL de cada PM, se sembraron en agar MRS (Man, Rogosa y Sharpe) y se incubaron a 37 ºC durante 72 h bajo condiciones anaerobias (AnaeroGen, Oxoid). Las cepas de BAL son catalasa y oxidasa negativas, cocos, coco-bacilos y bacilos Gram positivos no esporulados. La identificación bioquímica se realizó con el sistema API-50CHL (BioMérieux, France) y la base de datos APILAB Plus versión 3.3.3. Se aislaron 39 cepas de BAL, 4 del PMSI (2 Lactobacillus plantarum, 1 L. brevis y 1 Lactococcus lactis), 12 del PMSL (3 Lactobacillus rhamnosus, 3 L. casei, 2 L. acidophilus, 2 L. paracasei y 2 L. fermentum), 7 del PMEB (3 Streptococcus bovis, 2 Lactobacillus plantarum y 2 Leuconostoc mesenteroides), 10 del PMM (3 Lactobacillus casei, 2 L. plantarum, 2 L. acidophilus, 1 L. fermentum, 1 L. rhamnosus y 1 L. paracasei) y 6 del PMCR (2 Lactobacillus vitulinus, 2 L. ruminus, 1 L. reuteri y 1 Pediococcus damnosus). En conclusión, la mejor fuente de cepas de BAL fue PMSL, que le otorga ventajas como posible inoculante bacteriano para ensilajes. En todos los PM se identificaron cepas homofermentativas y heterofermentativas, asociadas en consorcios microbianos, se generó una alternativa tecnológica para aprovechar tres residuos agroindustriales. Universidad Pedagógica y Tecnológica de Colombia 2014-01-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion investigation application/pdf https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/3484 10.19053/01228420.3484 Ciencia y Agricultura; Vol. 11 No. 1 (2014); 17-25 Ciencia y Agricultura; Vol. 11 Núm. 1 (2014); 17-25 2539-0899 spa https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/3484/3104 Copyright (c) 2015 CIENCIA Y AGRICULTURA |
| spellingShingle | lactic acid bacteria LAB microbial preparation MP whey bovine manure ruminal content Bacterias ácido lácticas BAL Preparado microbiano PM Suero de leche Estiércol bovino Contenido ruminal. Díaz-Monroy, Byron Leoncio Iglesias, Arabel Elías Valiño-Cabrera, Elaine Microbial Consortiums with Promissory Lactic Acid Activity, Isolated from Native Bacterial Inoculants for Silages |
| title | Microbial Consortiums with Promissory Lactic Acid Activity, Isolated from Native Bacterial Inoculants for Silages |
| title_alt | Consorcios microbianos con actividad ácido-láctica promisoria aislados desde inoculantes bacterianos nativos para ensilajes |
| title_full | Microbial Consortiums with Promissory Lactic Acid Activity, Isolated from Native Bacterial Inoculants for Silages |
| title_fullStr | Microbial Consortiums with Promissory Lactic Acid Activity, Isolated from Native Bacterial Inoculants for Silages |
| title_full_unstemmed | Microbial Consortiums with Promissory Lactic Acid Activity, Isolated from Native Bacterial Inoculants for Silages |
| title_short | Microbial Consortiums with Promissory Lactic Acid Activity, Isolated from Native Bacterial Inoculants for Silages |
| title_sort | microbial consortiums with promissory lactic acid activity isolated from native bacterial inoculants for silages |
| topic | lactic acid bacteria LAB microbial preparation MP whey bovine manure ruminal content Bacterias ácido lácticas BAL Preparado microbiano PM Suero de leche Estiércol bovino Contenido ruminal. |
| topic_facet | lactic acid bacteria LAB microbial preparation MP whey bovine manure ruminal content Bacterias ácido lácticas BAL Preparado microbiano PM Suero de leche Estiércol bovino Contenido ruminal. |
| url | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/3484 |
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