In vitro effect of yeast preparations on ruminal methane production
The aim of this study was to evaluate the effect, in vitro, of Saccharomyces cerevisiae and Levica -25 preparations, viables on ruminal methanogens and methanogenesis. Three treatments were compared: C) Cynodon nlemfuensis (Star grass) as control; S) C. nlemfuensis + S. cerevisiae; and L) Levica 25...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2014
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/3836 |
| _version_ | 1801706464155271168 |
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| author | Díaz-Monroy, Byron Leoncio Castañeda-Caguana, Sandra Isabel |
| author_facet | Díaz-Monroy, Byron Leoncio Castañeda-Caguana, Sandra Isabel |
| author_sort | Díaz-Monroy, Byron Leoncio |
| collection | OJS |
| description | The aim of this study was to evaluate the effect, in vitro, of Saccharomyces cerevisiae and Levica -25 preparations, viables on ruminal methanogens and methanogenesis. Three treatments were compared: C) Cynodon nlemfuensis (Star grass) as control; S) C. nlemfuensis + S. cerevisiae; and L) Levica 25 + C. nlemfuensis, all with rumen fluid from adult male buffalo. The treatments were evaluated using standard laboratory techniques for microbiological and fermentation indicators at 8, 12 and 24 hours of fermentation, under completely randomized design with 3x3 bifactorial arrangement (3 treatments with 3 sampling times), with 4 replicates for each treatment, and a total of 36 experimental units represented by glass bottles of 100 mL each. We observed statistical differences (P =< 0.01) between treatments and between times assessed for the following indicators: methanogens population, methane gas production, cellulolytic bacteria, total viable bacteria, protozoa and pH. Yeast as a supplement in ruminants diet improves the food usage, increasing the cellulolytic bacteria population and decreasing the methanogenic bacteria population and enteric gas, which is an interesting contribution for mitigation environmental pollution. The yeast Levica -25 was more efficient. We recommend using Saccharomyces cerevisiae and Levica -25 preparations in large ruminants to decrease methanogenesis in the rumen and increase the population of cellulolytic bacteria. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-3836 |
| institution | Revista Ciencia y Agricultura |
| language | spa |
| publishDate | 2014 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-38362018-11-21T00:14:17Z In vitro effect of yeast preparations on ruminal methane production Efecto in vitro de preparados con levaduras sobre la producción de metano ruminal Díaz-Monroy, Byron Leoncio Castañeda-Caguana, Sandra Isabel cellulolytic yeast methanogenesis methanogenic ruminal. Celulolítico Levadura Metanogénesis Metanógeno Ruminal. The aim of this study was to evaluate the effect, in vitro, of Saccharomyces cerevisiae and Levica -25 preparations, viables on ruminal methanogens and methanogenesis. Three treatments were compared: C) Cynodon nlemfuensis (Star grass) as control; S) C. nlemfuensis + S. cerevisiae; and L) Levica 25 + C. nlemfuensis, all with rumen fluid from adult male buffalo. The treatments were evaluated using standard laboratory techniques for microbiological and fermentation indicators at 8, 12 and 24 hours of fermentation, under completely randomized design with 3x3 bifactorial arrangement (3 treatments with 3 sampling times), with 4 replicates for each treatment, and a total of 36 experimental units represented by glass bottles of 100 mL each. We observed statistical differences (P =< 0.01) between treatments and between times assessed for the following indicators: methanogens population, methane gas production, cellulolytic bacteria, total viable bacteria, protozoa and pH. Yeast as a supplement in ruminants diet improves the food usage, increasing the cellulolytic bacteria population and decreasing the methanogenic bacteria population and enteric gas, which is an interesting contribution for mitigation environmental pollution. The yeast Levica -25 was more efficient. We recommend using Saccharomyces cerevisiae and Levica -25 preparations in large ruminants to decrease methanogenesis in the rumen and increase the population of cellulolytic bacteria. El objetivo de este trabajo fue evaluar el efecto, in vitro, de preparados con levaduras Saccharomyces cerevisiae y Levica -25 viables sobre los metanógenos y la metanogénesis ruminal. Se compararon 3 tratamientos: C) Cynodon nlemfuensis (Pasto estrella), como control, S) Cynodon nlemfuensis + Saccharomyces cerevisiae, y L) Cynodon nlemfuensis + Levica 25, todos con líquido ruminal de búfalo adulto macho, y se valoraron mediante técnicas estándares de laboratorio indicadores microbiológicos y fermentativos a las 8, 12 y 24 horas de fermentación, bajo diseño completamente aleatorizado en arreglo bifactorial 3x3 (3 tratamientos con 3 horarios de muestreo), con 4 repeticiones para cada tratamiento, en total 36 unidades experimentales representadas por botellas de vidrio de 100 mL cada una. Existieron diferencias estadísticas (P≤0.01) entre tratamientos y entre horarios evaluados para los siguientes indicadores: población de metanógenos, producción de gas metano, bacterias celulolíticas, bacterias viables totales, protozoos y pH. La utilización de levaduras como suplemento en la dieta de rumiantes mejora el aprovechamiento del alimento, con incremento de la población de bacterias celulolíticas y disminución de la población de bacterias metanogénicas y gas entérico, lo cual es un aporte interesante a la mitigación de la contaminación ambiental. La levadura Levica-25 resultó más eficiente. Se recomienda utilizar preparados de Saccharomyces cerevisiae y Levica 25 en rumiantes mayores para disminuir la metanogénesis en el rumen e incrementar la población de bacterias celulolíticas. Universidad Pedagógica y Tecnológica de Colombia 2014-07-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion investigation application/pdf https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/3836 10.19053/01228420.3836 Ciencia y Agricultura; Vol. 11 No. 2 (2014); 39-47 Ciencia y Agricultura; Vol. 11 Núm. 2 (2014); 39-47 2539-0899 spa https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/3836/3376 Copyright (c) 2015 CIENCIA Y AGRICULTURA |
| spellingShingle | cellulolytic yeast methanogenesis methanogenic ruminal. Celulolítico Levadura Metanogénesis Metanógeno Ruminal. Díaz-Monroy, Byron Leoncio Castañeda-Caguana, Sandra Isabel In vitro effect of yeast preparations on ruminal methane production |
| title | In vitro effect of yeast preparations on ruminal methane production |
| title_alt | Efecto in vitro de preparados con levaduras sobre la producción de metano ruminal |
| title_full | In vitro effect of yeast preparations on ruminal methane production |
| title_fullStr | In vitro effect of yeast preparations on ruminal methane production |
| title_full_unstemmed | In vitro effect of yeast preparations on ruminal methane production |
| title_short | In vitro effect of yeast preparations on ruminal methane production |
| title_sort | in vitro effect of yeast preparations on ruminal methane production |
| topic | cellulolytic yeast methanogenesis methanogenic ruminal. Celulolítico Levadura Metanogénesis Metanógeno Ruminal. |
| topic_facet | cellulolytic yeast methanogenesis methanogenic ruminal. Celulolítico Levadura Metanogénesis Metanógeno Ruminal. |
| url | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/3836 |
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