Bos Taurus cattle sperm integrity effect by cryopreserved semen manpulation
This study evaluated the semen thawing and application effect in 33 bovine cattle farms in the Colombian Magdalena Medio region and studied in vitro, the injury effect found on the integrity of sperm membranes. The information was collected in farms by a specific paper form, The in vitro study was c...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2016
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4802 |
| _version_ | 1801706467759226880 |
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| author | Villa-Duque, Norberto Amaya-Torres, Claudia Marcela García-Rojas, Darwin Nieto-Omeara, Natalia Terán-Acuña, Natalia |
| author_facet | Villa-Duque, Norberto Amaya-Torres, Claudia Marcela García-Rojas, Darwin Nieto-Omeara, Natalia Terán-Acuña, Natalia |
| author_sort | Villa-Duque, Norberto |
| collection | OJS |
| description | This study evaluated the semen thawing and application effect in 33 bovine cattle farms in the Colombian Magdalena Medio region and studied in vitro, the injury effect found on the integrity of sperm membranes. The information was collected in farms by a specific paper form, The in vitro study was carried out in the Reproductive Animal Biotechnology laboratory at the Instituto Universitario de la Paz (Barrancabermeja, Santander, Colombia). The study consisted of exposing commercial 0.5 ml straws of bull Holstein and Brown Swiss to the conventional technique, and three modifications thereof (injuries) by a randomized design. None of the farms evaluated applied correctly the practice of artificial insemination. Evident errors were: Time excess for extraction of the straw, thawing in the axillary region and incorrect combination of time and temperature. The results showed significant difference (P <0.05) by the race effect for the integrity and strength of the plasma membrane, also for the integrity ofthe membranes by treatment effect, when the straw was thawed at body temperature in the axillary region and for the integrity of the acrosomal membrane when extraction straw was performed incorrectly. Holstein semen evidences a slight tendency to be more resistant than the Brown Swiss breed. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-4802 |
| institution | Revista Ciencia y Agricultura |
| language | spa |
| publishDate | 2016 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-48022018-11-21T00:10:13Z Bos Taurus cattle sperm integrity effect by cryopreserved semen manpulation Efecto de la manipulación del semen criopreservado de bovinos Bos Taurus sobre la integridad espermática Villa-Duque, Norberto Amaya-Torres, Claudia Marcela García-Rojas, Darwin Nieto-Omeara, Natalia Terán-Acuña, Natalia acrosome aitificial insemination genotype plasma reproductive biotechnology. acrosoma biotecnología reproductiva genotipo inseminación artificial plasmática This study evaluated the semen thawing and application effect in 33 bovine cattle farms in the Colombian Magdalena Medio region and studied in vitro, the injury effect found on the integrity of sperm membranes. The information was collected in farms by a specific paper form, The in vitro study was carried out in the Reproductive Animal Biotechnology laboratory at the Instituto Universitario de la Paz (Barrancabermeja, Santander, Colombia). The study consisted of exposing commercial 0.5 ml straws of bull Holstein and Brown Swiss to the conventional technique, and three modifications thereof (injuries) by a randomized design. None of the farms evaluated applied correctly the practice of artificial insemination. Evident errors were: Time excess for extraction of the straw, thawing in the axillary region and incorrect combination of time and temperature. The results showed significant difference (P <0.05) by the race effect for the integrity and strength of the plasma membrane, also for the integrity ofthe membranes by treatment effect, when the straw was thawed at body temperature in the axillary region and for the integrity of the acrosomal membrane when extraction straw was performed incorrectly. Holstein semen evidences a slight tendency to be more resistant than the Brown Swiss breed. En el estudio se evaluó el efecto de descongelar y aplicar semen de bovinos Bos Taurus en 33 ganaderías del Magdalena Medio colombiano, y se estudió in vitro el efecto de la injuria encontrada sobre la integridad de las membranas espermáticas. La información en fincas se recopiló mediante formulario específico, mientras que el estudio in vitro se ejecutó en el laboratorio de Biotecnología Reproductiva Animal del Instituto Universitario de la Paz (Barrancabermeja, Santander). El estudio consistió en someter pajillas comerciales de 0.5 ml de toros Holstein y Pardo Suizo a la técnica convencional y a tres modificaciones de esta (injurias) mediante un diseño randomizado. Ninguna de las fincas evaluadas aplicó correctamente la práctica de la inseminación artificial, errores notorios fueron: exceso de tiempo durante la extracción de la pajilla, descongelación en la región axilar y no combinación correcta entre tiempo y temperatura. Los resultados evidenciaron diferencia significativa (P&lt,0.05) por efecto de la raza para la integridad y resistencia de las membranas espermáticas, para la integridad de las membranas por efecto de los tratamientos cuando la pajilla se descongelo a temperatura corporal en la región axilar y para la integridad de la membrana acrosomal cuando la extracción de la pajilla se realizó en forma incorrecta. El semen de la raza Holstein evidencia una ligera tendencia a ser más resistente que el de la raza Pardo Suizo. Universidad Pedagógica y Tecnológica de Colombia 2016-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion investigation application/pdf https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4802 10.19053/01228420.4802 Ciencia y Agricultura; Vol. 13 No. 1 (2016); 9-18 Ciencia y Agricultura; Vol. 13 Núm. 1 (2016); 9-18 2539-0899 spa https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4802/3868 Copyright (c) 2016 CIENCIA Y AGRICULTURA |
| spellingShingle | acrosome aitificial insemination genotype plasma reproductive biotechnology. acrosoma biotecnología reproductiva genotipo inseminación artificial plasmática Villa-Duque, Norberto Amaya-Torres, Claudia Marcela García-Rojas, Darwin Nieto-Omeara, Natalia Terán-Acuña, Natalia Bos Taurus cattle sperm integrity effect by cryopreserved semen manpulation |
| title | Bos Taurus cattle sperm integrity effect by cryopreserved semen manpulation |
| title_alt | Efecto de la manipulación del semen criopreservado de bovinos Bos Taurus sobre la integridad espermática |
| title_full | Bos Taurus cattle sperm integrity effect by cryopreserved semen manpulation |
| title_fullStr | Bos Taurus cattle sperm integrity effect by cryopreserved semen manpulation |
| title_full_unstemmed | Bos Taurus cattle sperm integrity effect by cryopreserved semen manpulation |
| title_short | Bos Taurus cattle sperm integrity effect by cryopreserved semen manpulation |
| title_sort | bos taurus cattle sperm integrity effect by cryopreserved semen manpulation |
| topic | acrosome aitificial insemination genotype plasma reproductive biotechnology. acrosoma biotecnología reproductiva genotipo inseminación artificial plasmática |
| topic_facet | acrosome aitificial insemination genotype plasma reproductive biotechnology. acrosoma biotecnología reproductiva genotipo inseminación artificial plasmática |
| url | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4802 |
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