Lactobacillus spp. effect evaluation in the small intestine development in broiler chickens

The probiotic Lactobacillus spp. type, are considered promising alternatives to replace the use of antibiotics as growth promoters (APC) in poultry farms, due to their influence in the gastrointestinal tract (GIT) poseclosion development and in their microbial ecology. Thereof in this study the morp...

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Main Authors: Rodríguez-González, Sandra Paola, Moreno-Figueredo, Giovanni
Format: Online
Language:spa
Published: Universidad Pedagógica y Tecnológica de Colombia 2016
Subjects:
Online Access:https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4805
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author Rodríguez-González, Sandra Paola
Moreno-Figueredo, Giovanni
author_facet Rodríguez-González, Sandra Paola
Moreno-Figueredo, Giovanni
author_sort Rodríguez-González, Sandra Paola
collection OJS
description The probiotic Lactobacillus spp. type, are considered promising alternatives to replace the use of antibiotics as growth promoters (APC) in poultry farms, due to their influence in the gastrointestinal tract (GIT) poseclosion development and in their microbial ecology. Thereof in this study the morphometry of the small intestine in broilers to provide with 0.5 mL/L of a commercial product based on Lactobacillus spp. were evaluated. 200 male chickens a day old, from the Cobb Avian 48 line were used, distributed under a completely randomized design, in two groups, called control and experiment. Both groups were kept in similar conditions of feeding, management and health care.At 7, 15 and 35 days old sacrifice and necropsy was performed. Segments from duodenum, jejunum and ileum were dissected for processing with the hematoxylin-eosin (H-E) coloration technique. The villi morphometric changes in length, width and number were assessed using a trinocular microscope with digital camera Moticam®. Data were analyzed using SPSS version 19 program. Comparisons of averages were conducted through descriptive statistics and ANOVA (p < 0.05) test. Significant differences (p <0.05l were estimated in favor ofthe poseclosion development experiment group ofthe jejunum and ileum segments.
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spelling oai:oai.revistas.uptc.edu.co:article-48052018-11-21T00:10:00Z Lactobacillus spp. effect evaluation in the small intestine development in broiler chickens Evaluación del efecto de Lactobacillus spp. en el desarrollo del intestino delgado en pollos de engorde Rodríguez-González, Sandra Paola Moreno-Figueredo, Giovanni broilers chicken intestine development lactobacillus promoter villi. desarrollo intestino Lactobacillus pollos de engorde promotor vellosidad (DeCS) The probiotic Lactobacillus spp. type, are considered promising alternatives to replace the use of antibiotics as growth promoters (APC) in poultry farms, due to their influence in the gastrointestinal tract (GIT) poseclosion development and in their microbial ecology. Thereof in this study the morphometry of the small intestine in broilers to provide with 0.5 mL/L of a commercial product based on Lactobacillus spp. were evaluated. 200 male chickens a day old, from the Cobb Avian 48 line were used, distributed under a completely randomized design, in two groups, called control and experiment. Both groups were kept in similar conditions of feeding, management and health care.At 7, 15 and 35 days old sacrifice and necropsy was performed. Segments from duodenum, jejunum and ileum were dissected for processing with the hematoxylin-eosin (H-E) coloration technique. The villi morphometric changes in length, width and number were assessed using a trinocular microscope with digital camera Moticam®. Data were analyzed using SPSS version 19 program. Comparisons of averages were conducted through descriptive statistics and ANOVA (p < 0.05) test. Significant differences (p <0.05l were estimated in favor ofthe poseclosion development experiment group ofthe jejunum and ileum segments. Los probióticos del tipo Lactobacillus spp. son considerados alternativas promisorias para reemplazar los antibióticos como promotores de crecimiento (APC) en las explotaciones avícolas, ya que influyen en el desarrollo poseclosión del tracto gastrointestinal (TGI) y en su ecología microbiana. En este estudio se evaluó la morfometría del intestino delgado en pollos de engorde al suministrar 0.5mL/L de un producto comercial a base de Lactobacillus spp. Se utilizaron 200 pollos machos de un día de edad, de la línea Avian Cobb 48, los cuales se distribuyeron, con un diseño completamente al azar, en dos grupos, denominados control y experimento, ambos grupos fueron mantenidos en condiciones similares de alimentación, manejo y sanidad. A los días 7, 15 y 35 de edad se realizó el sacrificio y la necropsia, se disectaron segmentos de duodeno, yeyuno e íleon para su procesamiento con la técnica de coloración de Hematoxilina-Eosina (HE). Los cambios morfométricos de longitud, ancho y cantidad de vellosidades fueron evaluados utilizando un microscopio trinocular con cámara digital Moticam®. Los datos se analizaron usando el programa SPSS versión 19, se realizaron comparaciones de los promedios a través de estadística descriptiva y una prueba ANOVA (p&lt,0,05), se observaron diferencias estimadas significativas (p&lt,0,05) a favor del grupo experimento en el desarrollo poseclosión de los segmentos yeyuno e íleon. Universidad Pedagógica y Tecnológica de Colombia 2016-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion investigation application/pdf https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4805 10.19053/01228420.4805 Ciencia y Agricultura; Vol. 13 No. 1 (2016); 49-58 Ciencia y Agricultura; Vol. 13 Núm. 1 (2016); 49-58 2539-0899 spa https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4805/3871 Copyright (c) 2016 CIENCIA Y AGRICULTURA
spellingShingle broilers chicken
intestine development
lactobacillus
promoter
villi.
desarrollo intestino
Lactobacillus
pollos de engorde
promotor
vellosidad (DeCS)
Rodríguez-González, Sandra Paola
Moreno-Figueredo, Giovanni
Lactobacillus spp. effect evaluation in the small intestine development in broiler chickens
title Lactobacillus spp. effect evaluation in the small intestine development in broiler chickens
title_alt Evaluación del efecto de Lactobacillus spp. en el desarrollo del intestino delgado en pollos de engorde
title_full Lactobacillus spp. effect evaluation in the small intestine development in broiler chickens
title_fullStr Lactobacillus spp. effect evaluation in the small intestine development in broiler chickens
title_full_unstemmed Lactobacillus spp. effect evaluation in the small intestine development in broiler chickens
title_short Lactobacillus spp. effect evaluation in the small intestine development in broiler chickens
title_sort lactobacillus spp effect evaluation in the small intestine development in broiler chickens
topic broilers chicken
intestine development
lactobacillus
promoter
villi.
desarrollo intestino
Lactobacillus
pollos de engorde
promotor
vellosidad (DeCS)
topic_facet broilers chicken
intestine development
lactobacillus
promoter
villi.
desarrollo intestino
Lactobacillus
pollos de engorde
promotor
vellosidad (DeCS)
url https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4805
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AT morenofigueredogiovanni lactobacillussppeffectevaluationinthesmallintestinedevelopmentinbroilerchickens
AT rodriguezgonzalezsandrapaola evaluaciondelefectodelactobacillussppeneldesarrollodelintestinodelgadoenpollosdeengorde
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