Effect evaluation of artemrsinins from ethanolic extract of Artemisia cina against L3 of Haemonchus contortus on a abomasal explants technique
The new approach of integrated nematode control has driven to look for other alternatives that consider steadily and sustainable the environment. The aim of the present study was to obtain different ethanolic fracctions from Artemisia cina plant, using artemisinins, and to evaluate the nematicide as...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2016
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4810 |
| _version_ | 1801706468784734208 |
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| author | Higuera-Piedrahita, Rosa Isabel López-Arellano, María Eugenia López-Arellano, Raquel Cuenca-Verde, César Cuéllar-Ordaz, Jorge Alfredo |
| author_facet | Higuera-Piedrahita, Rosa Isabel López-Arellano, María Eugenia López-Arellano, Raquel Cuenca-Verde, César Cuéllar-Ordaz, Jorge Alfredo |
| author_sort | Higuera-Piedrahita, Rosa Isabel |
| collection | OJS |
| description | The new approach of integrated nematode control has driven to look for other alternatives that consider steadily and sustainable the environment. The aim of the present study was to obtain different ethanolic fracctions from Artemisia cina plant, using artemisinins, and to evaluate the nematicide association capacity effect with infective larvae (L3) and abomasal explant. The artemisinins from A. cina were obtained by thin layer chromatography and were evaluated on unsheathed L3 of Haemonchus contortus. The abomasal technique explants were performed for triplicate and compared as follows: three control with levamisole (7.5 mg/ml), commercial artemisinin (1 mg/ml), and distillate water, and six A. cina fracctions at 1 mg/ml each one: Ac3k, Ac3b, Ac3a, Ac3h, Ac3i and Ac4b. No significant differences between fractions of A. cina and commercial artemisinin (p>0.05) were obtained. These findings led us to conclude that there was no association capacity effect of fractions of A. cina on unsheathed L3 of H. contortus, to the abomasal tissue. It is important to continue with more studies to determine the parasitic phase in which the artemisinin viability is susceptible to be affected. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-4810 |
| institution | Revista Ciencia y Agricultura |
| language | spa |
| publishDate | 2016 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-48102021-07-12T05:04:44Z Effect evaluation of artemrsinins from ethanolic extract of Artemisia cina against L3 of Haemonchus contortus on a abomasal explants technique Evaluación del efecto de las artemisininas provenientes del extracto etanólico de Artemisia cina sobre L3 de Haemonchus contortus en una técnica de explantes abomasales Higuera-Piedrahita, Rosa Isabel López-Arellano, María Eugenia López-Arellano, Raquel Cuenca-Verde, César Cuéllar-Ordaz, Jorge Alfredo abomasal explants artemisia cina aitemisinin ethanolic extract haemonchus contortus. Artemisia cina artemisinina extracto etanólico explantes abomasales Haemonchus contortus The new approach of integrated nematode control has driven to look for other alternatives that consider steadily and sustainable the environment. The aim of the present study was to obtain different ethanolic fracctions from Artemisia cina plant, using artemisinins, and to evaluate the nematicide association capacity effect with infective larvae (L3) and abomasal explant. The artemisinins from A. cina were obtained by thin layer chromatography and were evaluated on unsheathed L3 of Haemonchus contortus. The abomasal technique explants were performed for triplicate and compared as follows: three control with levamisole (7.5 mg/ml), commercial artemisinin (1 mg/ml), and distillate water, and six A. cina fracctions at 1 mg/ml each one: Ac3k, Ac3b, Ac3a, Ac3h, Ac3i and Ac4b. No significant differences between fractions of A. cina and commercial artemisinin (p>0.05) were obtained. These findings led us to conclude that there was no association capacity effect of fractions of A. cina on unsheathed L3 of H. contortus, to the abomasal tissue. It is important to continue with more studies to determine the parasitic phase in which the artemisinin viability is susceptible to be affected. El nuevo enfoque de control integrado de parásitos obliga a buscar alternativas que consideren el medioambiente, es decir, sostenibles y sustentables. El objetivo de este trabajo fue fraccionar el extracto etanólico de la planta Artemisia cina, obtener artemisininas y conocer el efecto de estas sobre la capacidad de asociación de las L3 de Haemonchus contortus en explantes abomasales. El extracto etanólico se fraccionó por medio de la metodología establecida para Artemisia japónica, y se identificaron las artemisininas por medio de cromatografía en capa fina, teniendo como referencia artemisininas comerciales. Las artemisininas se utilizaron sobre L3 desenvainada, se realizó la técnica de explantes abomasales por triplicado y se compararon así: levamisol (7.5 mg/ml), artemisinina comercial (1 mg/ml), agua y seis diferentes fracciones de A. cina a dosis de 1 mg/ml: Ac3k, Ac3b, Ac3a, Ac3h, Ac3i, Ac4b. No se obtuvieron diferencias significativas entre las fracciones de A. cina y artemisinina comercial (p&gt,0.05). Se concluye que no existió efecto de las artemisininas obtenidas del extracto etanólico de A. cina sobre la capacidad de asociación de las L3 de H. contortus al tejido abomasal. Es importante continuar con más estudios de la artemisinina para determinar sobre qué fase del parásito afecta su viabilidad. Universidad Pedagógica y Tecnológica de Colombia 2016-01-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion investigation application/pdf https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4810 10.19053/01228420.4810 Ciencia y Agricultura; Vol. 13 No. 1 (2016); 107-116 Ciencia y Agricultura; Vol. 13 Núm. 1 (2016); 107-116 2539-0899 spa https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4810/3876 Copyright (c) 2016 CIENCIA Y AGRICULTURA |
| spellingShingle | abomasal explants artemisia cina aitemisinin ethanolic extract haemonchus contortus. Artemisia cina artemisinina extracto etanólico explantes abomasales Haemonchus contortus Higuera-Piedrahita, Rosa Isabel López-Arellano, María Eugenia López-Arellano, Raquel Cuenca-Verde, César Cuéllar-Ordaz, Jorge Alfredo Effect evaluation of artemrsinins from ethanolic extract of Artemisia cina against L3 of Haemonchus contortus on a abomasal explants technique |
| title | Effect evaluation of artemrsinins from ethanolic extract of Artemisia cina against L3 of Haemonchus contortus on a abomasal explants technique |
| title_alt | Evaluación del efecto de las artemisininas provenientes del extracto etanólico de Artemisia cina sobre L3 de Haemonchus contortus en una técnica de explantes abomasales |
| title_full | Effect evaluation of artemrsinins from ethanolic extract of Artemisia cina against L3 of Haemonchus contortus on a abomasal explants technique |
| title_fullStr | Effect evaluation of artemrsinins from ethanolic extract of Artemisia cina against L3 of Haemonchus contortus on a abomasal explants technique |
| title_full_unstemmed | Effect evaluation of artemrsinins from ethanolic extract of Artemisia cina against L3 of Haemonchus contortus on a abomasal explants technique |
| title_short | Effect evaluation of artemrsinins from ethanolic extract of Artemisia cina against L3 of Haemonchus contortus on a abomasal explants technique |
| title_sort | effect evaluation of artemrsinins from ethanolic extract of artemisia cina against l3 of haemonchus contortus on a abomasal explants technique |
| topic | abomasal explants artemisia cina aitemisinin ethanolic extract haemonchus contortus. Artemisia cina artemisinina extracto etanólico explantes abomasales Haemonchus contortus |
| topic_facet | abomasal explants artemisia cina aitemisinin ethanolic extract haemonchus contortus. Artemisia cina artemisinina extracto etanólico explantes abomasales Haemonchus contortus |
| url | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/4810 |
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