Cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium cepa L.)

(NaClO) sodium hypochlorite is a highly toxic chemical compound widely used because of its disinfectant properties. One of the most commonly used plants to detect different types of genetic changes caused by chemical substances is Allium cepa L., in tests for environmental monitori...

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Detalhes bibliográficos
Principais autores: Causil V., Luis Alfonso, Coronado G., José Luis, Verbel M., Luis Fernando, Vega J., María Fernanda, Donado E., Keiner Antonio, Pacheco G., Carluis
Formato: Online
Idioma:spa
Publicado em: Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2017
Assuntos:
Acesso em linha:https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5662
Descrição
Resumo:(NaClO) sodium hypochlorite is a highly toxic chemical compound widely used because of its disinfectant properties. One of the most commonly used plants to detect different types of genetic changes caused by chemical substances is Allium cepa L., in tests for environmental monitoring. The objective of this research was to evaluate the cytotoxic effect of sodium hypochlorite (NaClO) in apical cells of onion roots (Allium strain L.). Five sodium hypochlorite solutions were prepared at different concentrations, in addition to a control solution, with three replications for each treatment; for 3 days, the growth of the roots was measured; the apices were cut submerging them into hydrochloric acid, subsequently dyed with Aceto-Orcein and were observed under a microscope; the mitotic index (MI) was calculated and the cell anomalies were observed (chromosomal). An analysis of variance (ANOVA) and multiple range tests using the Least Significant Difference method (LSD) were performed. The results showed that there was not a statistically significant difference between the treatments (P