Inoculation of indole-3-acetic acid producing bacteria in lettuce (Lactuca sativa L.) plants
Microorganisms are of great importance for the development of plants through the production of various compounds, such as indole-3-acetic acid (IAA), synthesized by bacteria. However, research on the effect of inoculation of these bacteria on plants is still lacking. The objective of this study was...
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| Format: | Online |
| Language: | por |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2017
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5780 |
| _version_ | 1801706270129913856 |
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| author | Florentino, Ligiane A. Silva, Adriano B. Landgraf, Paulo R. C. Souza, Flávia R. C. |
| author_facet | Florentino, Ligiane A. Silva, Adriano B. Landgraf, Paulo R. C. Souza, Flávia R. C. |
| author_sort | Florentino, Ligiane A. |
| collection | OJS |
| description | Microorganisms are of great importance for the development of plants through the production of various compounds, such as indole-3-acetic acid (IAA), synthesized by bacteria. However, research on the effect of inoculation of these bacteria on plants is still lacking. The objective of this study was to evaluate the in vitro production of IAA by diazotrophic bacteria grown in the presence or absence of tryptophan (Trp) and the effect of inoculation of these strains on lettuce seeds (Lactuca sativa L.). Initially, a completely randomized design experiment (DIC) was installed in a 19 × 2 factorial scheme, with 19 bacterial strains grown in media containing or not containing Trp, with four replications. It has been observed that Trp contributes to a higher production of IAA by most strains. Subsequently, six of these strains were selected for inoculation in lettuce seeds in two experiments: the first in gerbox and the other in trays containing commercial substrate. DIC was used in a 8 × 2 factorial scheme, with seven bacterial strains and one control without inoculation and the presence or absence of Trp in the culture medium, with four replications. Inoculation with most of the strains contributed to an increase in the seed germination rate, root length and shoot dry matter weight; however, the excess of IAA produced by some strains when grown in the presence of Trp may interfere with the development of lettuce seedlings. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-5780 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | por |
| publishDate | 2017 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-57802020-08-03T23:58:55Z Inoculation of indole-3-acetic acid producing bacteria in lettuce (Lactuca sativa L.) plants Inoculação de bactérias produtoras de ácido 3-indol acético em plantas de alface (Lactuca sativa L.) Florentino, Ligiane A. Silva, Adriano B. Landgraf, Paulo R. C. Souza, Flávia R. C. Phyto-hormones plant Growth promoting bacteria Tryptophan Sustainability Agriculture Fito-hormônios Bactérias promotoras do crescimento vegetal Triptofano Sustentabilidade Agriculture Agricultura Microorganisms are of great importance for the development of plants through the production of various compounds, such as indole-3-acetic acid (IAA), synthesized by bacteria. However, research on the effect of inoculation of these bacteria on plants is still lacking. The objective of this study was to evaluate the in vitro production of IAA by diazotrophic bacteria grown in the presence or absence of tryptophan (Trp) and the effect of inoculation of these strains on lettuce seeds (Lactuca sativa L.). Initially, a completely randomized design experiment (DIC) was installed in a 19 × 2 factorial scheme, with 19 bacterial strains grown in media containing or not containing Trp, with four replications. It has been observed that Trp contributes to a higher production of IAA by most strains. Subsequently, six of these strains were selected for inoculation in lettuce seeds in two experiments: the first in gerbox and the other in trays containing commercial substrate. DIC was used in a 8 × 2 factorial scheme, with seven bacterial strains and one control without inoculation and the presence or absence of Trp in the culture medium, with four replications. Inoculation with most of the strains contributed to an increase in the seed germination rate, root length and shoot dry matter weight; however, the excess of IAA produced by some strains when grown in the presence of Trp may interfere with the development of lettuce seedlings. Os microrganismos exercem grande importância para o desenvolvimento dos vegetais por meio da produção de diversos compostos, como o ácido 3-indol acético (AIA), sintetizado por bactérias. No entanto, ainda são incipientes as pesquisas relatando o efeito da inoculação destas bactérias nas plantas. Dessa forma, o objetivo desse estudo foi avaliar a produção in vitro de AIA por bactérias diazotróficas cultivadas na presença ou ausência do triptofano (Trp) e o efeito da inoculação destas estirpes em sementes de alface (Lactuca sativa L.). Inicialmente, foi instalado um experimento em delineamento inteiramente casualizado (DIC) em esquema fatorial 19 × 2, sendo 19 estirpes bacterianas cultivadas em meios contendo ou não o Trp, com quatro repetições. Foi observado que o Trp contribui para a maior produção de AIA pela maioria das estirpes. Posteriormente, seis dessas estirpes foram selecionadas para inoculação em sementes de alface em dois experimentos, o primeiro em gerbox e o outro em bandejas contendo substrato comercial. Foi utilizado o DIC em esquema fatorial 8 × 2, sendo sete estirpes bacterianas e um controle sem inoculação e presença ou ausência do Trp no meio de cultivo, com quatro repetições. A inoculação com a maioria das estirpes contribuiu para o aumento da taxa de germinação das sementes, comprimento radicular e peso da matéria seca da parte aérea, no entanto, o excesso de AIA produzido por algumas estirpes quando cultivadas na presença de Trp pode interferir no desenvolvimento das mudas de alface. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2017-06-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5780 10.17584/rcch.2017v11i1.5780 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No. 1 (2017); 89-96 Revista Colombiana de Ciencias Hortícolas; Vol. 11 Núm. 1 (2017); 89-96 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No 1 (2017); 89-96 Revista Colombiana de Ciencias Hortícolas; V. 11 N. 1 (2017); 89-96 Revista Colombiana de Ciencias Hortícolas; v. 11 n. 1 (2017); 89-96 2422-3719 2011-2173 por https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5780/pdf |
| spellingShingle | Phyto-hormones plant Growth promoting bacteria Tryptophan Sustainability Agriculture Fito-hormônios Bactérias promotoras do crescimento vegetal Triptofano Sustentabilidade Agriculture Agricultura Florentino, Ligiane A. Silva, Adriano B. Landgraf, Paulo R. C. Souza, Flávia R. C. Inoculation of indole-3-acetic acid producing bacteria in lettuce (Lactuca sativa L.) plants |
| title | Inoculation of indole-3-acetic acid producing bacteria in lettuce (Lactuca sativa L.) plants |
| title_alt | Inoculação de bactérias produtoras de ácido 3-indol acético em plantas de alface (Lactuca sativa L.) |
| title_full | Inoculation of indole-3-acetic acid producing bacteria in lettuce (Lactuca sativa L.) plants |
| title_fullStr | Inoculation of indole-3-acetic acid producing bacteria in lettuce (Lactuca sativa L.) plants |
| title_full_unstemmed | Inoculation of indole-3-acetic acid producing bacteria in lettuce (Lactuca sativa L.) plants |
| title_short | Inoculation of indole-3-acetic acid producing bacteria in lettuce (Lactuca sativa L.) plants |
| title_sort | inoculation of indole 3 acetic acid producing bacteria in lettuce lactuca sativa l plants |
| topic | Phyto-hormones plant Growth promoting bacteria Tryptophan Sustainability Agriculture Fito-hormônios Bactérias promotoras do crescimento vegetal Triptofano Sustentabilidade Agriculture Agricultura |
| topic_facet | Phyto-hormones plant Growth promoting bacteria Tryptophan Sustainability Agriculture Fito-hormônios Bactérias promotoras do crescimento vegetal Triptofano Sustentabilidade Agriculture Agricultura |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/5780 |
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