Comparison in vitro of cytocompatibility between fibroin and polypropylene biomaterials
This study evaluates the cell behavior of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture much fibroin material such as polypropylene, as the number of cells / sample was performed by the metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-d...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | eng |
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Universidad Pedagógica y Tecnológica de Colombia
2017
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| Online Access: | https://revistas.uptc.edu.co/index.php/ingenieria/article/view/6056 |
| _version_ | 1801706078216388608 |
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| author | Arboleda-Carvajal, Alejandro González, Julián Franco-Arias, Manuel Hernando Valladares-Torres, Liliana |
| author_facet | Arboleda-Carvajal, Alejandro González, Julián Franco-Arias, Manuel Hernando Valladares-Torres, Liliana |
| author_sort | Arboleda-Carvajal, Alejandro |
| collection | OJS |
| description | This study evaluates the cell behavior of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture much fibroin material such as polypropylene, as the number of cells / sample was performed by the metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol Bromide, MTT assay, using direct and indirect evidence of cytotoxicity. For direct and indirect testing of cytotoxicity in fibroin and polypropylene material, a statistical difference was found in the average number of live cells for fibroin sample regardless of the type of test (p<0.005). By the use of in vitro methods, it is shown that fibroin material has better cell behavior in terms of viability, compared with polypropylene. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-6056 |
| institution | Revista Facultad de Ingeniería |
| language | eng |
| publishDate | 2017 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-60562022-06-15T16:17:40Z Comparison in vitro of cytocompatibility between fibroin and polypropylene biomaterials Comparación in vitro de la citocompatibilidad entre los biomateriales fibroína y polipropileno Arboleda-Carvajal, Alejandro González, Julián Franco-Arias, Manuel Hernando Valladares-Torres, Liliana biomaterials cell enlargement fibroin propylene polymers silk Biomateriales Crecimiento celular Fibroína Polímeros Polipropileno Seda This study evaluates the cell behavior of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture much fibroin material such as polypropylene, as the number of cells / sample was performed by the metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol Bromide, MTT assay, using direct and indirect evidence of cytotoxicity. For direct and indirect testing of cytotoxicity in fibroin and polypropylene material, a statistical difference was found in the average number of live cells for fibroin sample regardless of the type of test (p<0.005). By the use of in vitro methods, it is shown that fibroin material has better cell behavior in terms of viability, compared with polypropylene. Este artículo evalúa el crecimiento de células Hela in vitro en presencia de fibroína y polipropileno. Con el objetivo de determinar la proliferación celular en presencia de estos dos biomateriales, se obtuvo el número de células/muestra por medio de la prueba de reducción metabólica del bromuro de 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol (MTT), utilizando evidencia de citotoxicidad directa e indirecta. Las pruebas directas e indirectas de citotoxicidad de fibroína y polipropileno mostraron una diferencia estadísticamente significativa en el promedio de células vivas para fibroína (p<0.005), sin importar el tipo de test. Los métodos in vitro utilizados en este estudio permitieron observar que la fibroína tiene un mejor comportamiento celular, en términos de viabilidad, comparada con el polipropileno. Universidad Pedagógica y Tecnológica de Colombia 2017-05-02 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion investigation investigación application/pdf application/xml https://revistas.uptc.edu.co/index.php/ingenieria/article/view/6056 10.19053/01211129.v26.n45.2017.6056 Revista Facultad de Ingeniería; Vol. 26 No. 45 (2017); 97-107 Revista Facultad de Ingeniería; Vol. 26 Núm. 45 (2017); 97-107 2357-5328 0121-1129 eng https://revistas.uptc.edu.co/index.php/ingenieria/article/view/6056/5589 https://revistas.uptc.edu.co/index.php/ingenieria/article/view/6056/6410 |
| spellingShingle | biomaterials cell enlargement fibroin propylene polymers silk Biomateriales Crecimiento celular Fibroína Polímeros Polipropileno Seda Arboleda-Carvajal, Alejandro González, Julián Franco-Arias, Manuel Hernando Valladares-Torres, Liliana Comparison in vitro of cytocompatibility between fibroin and polypropylene biomaterials |
| title | Comparison in vitro of cytocompatibility between fibroin and polypropylene biomaterials |
| title_alt | Comparación in vitro de la citocompatibilidad entre los biomateriales fibroína y polipropileno |
| title_full | Comparison in vitro of cytocompatibility between fibroin and polypropylene biomaterials |
| title_fullStr | Comparison in vitro of cytocompatibility between fibroin and polypropylene biomaterials |
| title_full_unstemmed | Comparison in vitro of cytocompatibility between fibroin and polypropylene biomaterials |
| title_short | Comparison in vitro of cytocompatibility between fibroin and polypropylene biomaterials |
| title_sort | comparison in vitro of cytocompatibility between fibroin and polypropylene biomaterials |
| topic | biomaterials cell enlargement fibroin propylene polymers silk Biomateriales Crecimiento celular Fibroína Polímeros Polipropileno Seda |
| topic_facet | biomaterials cell enlargement fibroin propylene polymers silk Biomateriales Crecimiento celular Fibroína Polímeros Polipropileno Seda |
| url | https://revistas.uptc.edu.co/index.php/ingenieria/article/view/6056 |
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