Response in root development of Arabidopsis thaliana to leaf extract of Moringa oleifera
The root growth response is mediated by hormones that participate in all stages of plant growth and development. A good root system guarantees anchoring and absorption of nutrients for the metabolic processes required in the developmental stages. Cytokinins (CK) and auxins (AIA) are hormones that pl...
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2017
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6131 |
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| author | Ortiz-Rojas, Luz Yineth Suárez-Botello, Jean Carlos Chaves-Bedoya, Giovanni |
| author_facet | Ortiz-Rojas, Luz Yineth Suárez-Botello, Jean Carlos Chaves-Bedoya, Giovanni |
| author_sort | Ortiz-Rojas, Luz Yineth |
| collection | OJS |
| description | The root growth response is mediated by hormones that participate in all stages of plant growth and development. A good root system guarantees anchoring and absorption of nutrients for the metabolic processes required in the developmental stages. Cytokinins (CK) and auxins (AIA) are hormones that play an important role in the development of the root system. Moringa oleifera has cytokinins, so the application of their extracts can have an effect on the development of the root system. In order to understand the root response to the Moringa oleiferaextract, we used an in vitro system with Arabidopsis thaliana and two experiment conditions. (A) Seeds germinated directly in MS medium with application of the Moringa oleifera extract at different dilutions, which determined how the primary root (PR) growth was affected. (B) Plants were germinated and grown for 8 days in the MS medium, without lateral roots (LR), and transferred to media with different dilutions of the extract. The results showed that (A) the best dilution of Moringa oleifera base extract (KOH 0.05 M) for the germination stimulation was seen in the dilutions of 1 mL, 1.5 mL and 2.0 mL, as well as the treatment with trans Zeathine riboside (ZR) with a concentration of 60 μM, and (B) the basic extract of Moringa oleifera in a ratio of 1.5: 0.5 (extract: KOH 0.05 M) was the best treatment for the development of the primary root (PR) and lateral roots (LR). Further studies are needed to determine the validity of these results in field. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-6131 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | spa |
| publishDate | 2017 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-61312020-08-03T23:58:34Z Response in root development of Arabidopsis thaliana to leaf extract of Moringa oleifera Respuesta en el desarrollo radicular de Arabidopsis thaliana al extracto foliar de Moringa oleifera Ortiz-Rojas, Luz Yineth Suárez-Botello, Jean Carlos Chaves-Bedoya, Giovanni Root architecture Cytokinin Primary root Lateral roots Arquitectura radicular Citoquininas Raíz primaria Raíces laterales The root growth response is mediated by hormones that participate in all stages of plant growth and development. A good root system guarantees anchoring and absorption of nutrients for the metabolic processes required in the developmental stages. Cytokinins (CK) and auxins (AIA) are hormones that play an important role in the development of the root system. Moringa oleifera has cytokinins, so the application of their extracts can have an effect on the development of the root system. In order to understand the root response to the Moringa oleiferaextract, we used an in vitro system with Arabidopsis thaliana and two experiment conditions. (A) Seeds germinated directly in MS medium with application of the Moringa oleifera extract at different dilutions, which determined how the primary root (PR) growth was affected. (B) Plants were germinated and grown for 8 days in the MS medium, without lateral roots (LR), and transferred to media with different dilutions of the extract. The results showed that (A) the best dilution of Moringa oleifera base extract (KOH 0.05 M) for the germination stimulation was seen in the dilutions of 1 mL, 1.5 mL and 2.0 mL, as well as the treatment with trans Zeathine riboside (ZR) with a concentration of 60 μM, and (B) the basic extract of Moringa oleifera in a ratio of 1.5: 0.5 (extract: KOH 0.05 M) was the best treatment for the development of the primary root (PR) and lateral roots (LR). Further studies are needed to determine the validity of these results in field. La respuesta del crecimiento de las raíces se encuentra mediado por fitorreguladores que participan en todas las etapas de crecimiento y desarrollo de la planta. Un adecuado sistema radicular garantiza el anclaje y absorción de nutrientes para los procesos metabólicos requeridos en sus etapas fenológicas. Las citoquininas (CK) y auxinas (AIA), son fitorreguladores que desempeñan un papel importante en el desarrollo del sistema radicular. Se ha reportado que las hojas de Moringa oleifera son particularmente ricas en zeatina, por lo que la aplicación de sus extractos puede tener efecto en el desarrollo radicular. Para entender de manera detallada la respuesta de las raíces al extracto de Moringa oleifera, se utilizó un sistema in vitro con Arabidopsis thaliana usando dos condiciones experimentales: (A) semillas germinadas directamente en medio MS con aplicación del extracto de Moringa oleifera a diferentes diluciones y (B) plantas germinadas y crecidas por 8 días en medio MS, sin raíces laterales (RL), transferidas a medios con diferentes diluciones del extracto. Los resultados obtenidos sugieren que (A) la mejor dilución del extracto básico (KOH 0,05 M) de Moringa oleifera para la estimulación de la germinación se presentó en las diluciones de 1 mL, 1,5 mL y 2,0 mL, al igual que el tratamiento con trans zeatina ribósido (ZR) con una concentración 60 μM, (B) el extracto básico de Moringa oleifera en una relación 1.5:0.5 (extracto:KOH 0,05 M), fue el mejor tratamiento para el desarrollo de la raíz principal (RP) y raíces laterales (RL). Se necesitan estudios adicionales para determinar la validez de estos resultados en campo. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2017-06-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6131 10.17584/rcch.2017v11i1.6131 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No. 1 (2017); 193-199 Revista Colombiana de Ciencias Hortícolas; Vol. 11 Núm. 1 (2017); 193-199 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No 1 (2017); 193-199 Revista Colombiana de Ciencias Hortícolas; V. 11 N. 1 (2017); 193-199 Revista Colombiana de Ciencias Hortícolas; v. 11 n. 1 (2017); 193-199 2422-3719 2011-2173 spa https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6131/pdf |
| spellingShingle | Root architecture Cytokinin Primary root Lateral roots Arquitectura radicular Citoquininas Raíz primaria Raíces laterales Ortiz-Rojas, Luz Yineth Suárez-Botello, Jean Carlos Chaves-Bedoya, Giovanni Response in root development of Arabidopsis thaliana to leaf extract of Moringa oleifera |
| title | Response in root development of Arabidopsis thaliana to leaf extract of Moringa oleifera |
| title_alt | Respuesta en el desarrollo radicular de Arabidopsis thaliana al extracto foliar de Moringa oleifera |
| title_full | Response in root development of Arabidopsis thaliana to leaf extract of Moringa oleifera |
| title_fullStr | Response in root development of Arabidopsis thaliana to leaf extract of Moringa oleifera |
| title_full_unstemmed | Response in root development of Arabidopsis thaliana to leaf extract of Moringa oleifera |
| title_short | Response in root development of Arabidopsis thaliana to leaf extract of Moringa oleifera |
| title_sort | response in root development of arabidopsis thaliana to leaf extract of moringa oleifera |
| topic | Root architecture Cytokinin Primary root Lateral roots Arquitectura radicular Citoquininas Raíz primaria Raíces laterales |
| topic_facet | Root architecture Cytokinin Primary root Lateral roots Arquitectura radicular Citoquininas Raíz primaria Raíces laterales |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6131 |
| work_keys_str_mv | AT ortizrojasluzyineth responseinrootdevelopmentofarabidopsisthalianatoleafextractofmoringaoleifera AT suarezbotellojeancarlos responseinrootdevelopmentofarabidopsisthalianatoleafextractofmoringaoleifera AT chavesbedoyagiovanni responseinrootdevelopmentofarabidopsisthalianatoleafextractofmoringaoleifera AT ortizrojasluzyineth respuestaeneldesarrolloradiculardearabidopsisthalianaalextractofoliardemoringaoleifera AT suarezbotellojeancarlos respuestaeneldesarrolloradiculardearabidopsisthalianaalextractofoliardemoringaoleifera AT chavesbedoyagiovanni respuestaeneldesarrolloradiculardearabidopsisthalianaalextractofoliardemoringaoleifera |
