Evaluation of calcium carbonate (CaCO3) inclusion in solid-state kinetic fermentation of Solanum tuberosum postharvest waste

The objective of this study was to evaluate the pH behavior with CaCO3 inclusions in solid state fermentation of Solanum tuberosum postharvest waste inoculated with a microbial preparation. A supplement was obtained that can be used in animal feed with the following condit...

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Main Authors: Borras, Luis Miguel, Rodríguez, Carlos Eduardo, Rodríguez, Ángela
Format: Online
Language:spa
Published: Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2017
Subjects:
Online Access:https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6145
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author Borras, Luis Miguel
Rodríguez, Carlos Eduardo
Rodríguez, Ángela
author_facet Borras, Luis Miguel
Rodríguez, Carlos Eduardo
Rodríguez, Ángela
author_sort Borras, Luis Miguel
collection OJS
description The objective of this study was to evaluate the pH behavior with CaCO3 inclusions in solid state fermentation of Solanum tuberosum postharvest waste inoculated with a microbial preparation. A supplement was obtained that can be used in animal feed with the following conditions: 89% S. tuberosum, 5% fibrous material (wheat bran); 2% molasses, 1% urea; 2% of the microbial preparation (developed in the research), 0.5% premixed mineral and 0.5% of sodium sulphate. Four treatments were evaluated with 0, 0.25, 0.50 and 0.75% inclusion of CaCO3 to the above mixture at different temperatures (20 and 25ºC), and incubated at different fermentation times (24 and 48 hours). Analysis of variance was carried out according to a completely randomized design with a factorial arrangement (2×2×4) where the factors were two temperatures 20 and 25ºC, two fermentation times 24 and 48 hours and four inclusion levels of calcium carbonate and three replications. A Duncan test was applied for P≤0.05 when necessary. The INFOSTAT statistical package, version 2012, was used for the analysis. The influence of calcium carbonate on pH and MS (P<0.0001) was observed in the study. The pH increased as the percentage of CaCO3 addition supplied for both incubation temperatures increased for 48 hours, according to the values obtained in the fermentation kinetics, with the exception of 25ºC in the highest inclusion level (0.75%). For the DM, an increase in the different inclusion levels of calcium carbonate was observed at a temperature of 20ºC during fermentation although the highest value reached 39.66%. The two protein indicators showed differences for all levels of carbonate inclusion with respect to time and temperatures. However, the crude protein levels were maintained in comparison with those in the microbial preparation, obtaining the highest value with the inclusion of 0.50% CaCO3 at 20ºC, with a marked difference with protein tenors at 25ºC, which were always below the fermentation percentage at 20ºC. The best inclusion of CaCO3 in the fermentation of the postharvest waste of Solanum tuberosum with the microbial preparation was 0.50% at 20ºC, which resulted in an adequate growth of the BAL microorganisms, as reflected in the protein increase.
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spelling oai:oai.revistas.uptc.edu.co:article-61452020-08-03T23:58:39Z Evaluation of calcium carbonate (CaCO3) inclusion in solid-state kinetic fermentation of Solanum tuberosum postharvest waste Inclusión de carbonato de calcio (CaCO3) en la cinética de fermentación en estado sólido de residuos de poscosecha de Solanum tuberosum Borras, Luis Miguel Rodríguez, Carlos Eduardo Rodríguez, Ángela pH Microbiological analysis Lactic acid Bromatology Agriculture pH Análisis microbiológico Ácido láctico Bromatología Agricultura The objective of this study was to evaluate the pH behavior with CaCO3 inclusions in solid state fermentation of Solanum tuberosum postharvest waste inoculated with a microbial preparation. A supplement was obtained that can be used in animal feed with the following conditions: 89% S. tuberosum, 5% fibrous material (wheat bran); 2% molasses, 1% urea; 2% of the microbial preparation (developed in the research), 0.5% premixed mineral and 0.5% of sodium sulphate. Four treatments were evaluated with 0, 0.25, 0.50 and 0.75% inclusion of CaCO3 to the above mixture at different temperatures (20 and 25ºC), and incubated at different fermentation times (24 and 48 hours). Analysis of variance was carried out according to a completely randomized design with a factorial arrangement (2×2×4) where the factors were two temperatures 20 and 25ºC, two fermentation times 24 and 48 hours and four inclusion levels of calcium carbonate and three replications. A Duncan test was applied for P≤0.05 when necessary. The INFOSTAT statistical package, version 2012, was used for the analysis. The influence of calcium carbonate on pH and MS (P<0.0001) was observed in the study. The pH increased as the percentage of CaCO3 addition supplied for both incubation temperatures increased for 48 hours, according to the values obtained in the fermentation kinetics, with the exception of 25ºC in the highest inclusion level (0.75%). For the DM, an increase in the different inclusion levels of calcium carbonate was observed at a temperature of 20ºC during fermentation although the highest value reached 39.66%. The two protein indicators showed differences for all levels of carbonate inclusion with respect to time and temperatures. However, the crude protein levels were maintained in comparison with those in the microbial preparation, obtaining the highest value with the inclusion of 0.50% CaCO3 at 20ºC, with a marked difference with protein tenors at 25ºC, which were always below the fermentation percentage at 20ºC. The best inclusion of CaCO3 in the fermentation of the postharvest waste of Solanum tuberosum with the microbial preparation was 0.50% at 20ºC, which resulted in an adequate growth of the BAL microorganisms, as reflected in the protein increase. El objetivo del trabajo es evaluar el comportamiento del pH por medio de inclusiones de CaCO3 dentro de la fermentación en estado sólido de residuos de poscosecha de Solanum tuberosum inoculado con un preparado microbial; se obtuvo un suplemento que puede ser utilizado en la alimentación animal con las siguientes condiciones: 89% de S. tuberosum, 5% material fibroso (salvado de trigo); 2% de melaza, 1% de urea; 2% del preparado microbiano (desarrollado en la investigación), 0,5% premezcla mineral y 0,5% de sulfato de sodio. Se evaluaron cuatro tratamientos con 0; 0,25; 0,50 y 0,75% de inclusión del CaCO3 a la mezcla anterior a diferentes temperaturas (20 y 25ºC), e incubadas a diferentes tiempos de fermentación (24 y 48 horas). Se realizó análisis de varianza según diseño completamente aleatorizado con arreglo factorial (2×2×4) donde los factores fueron dos temperaturas 20 y 25ºC, dos tiempos de fermentación 24 y 48 horas y cuatro niveles de inclusión de carbonato de calcio y tres repeticiones. Se aplicó dócima de Duncan para P≤0,05 en los casos necesarios. Para los análisis se utilizó el paquete estadístico INFOSTAT, versión 2012. Se muestra en el estudio la influencia del carbonato de calcio, en el pH y la MS (P<0,0001). El pH se incrementa a medida que aumenta el porcentaje de adición del CaCo3suministrado para ambas temperaturas de incubación en 48 horas, según los valores obtenidos en la cinética de fermentación, exceptuando a los 25ºC en el nivel más alto de inclusión (0,75%), Con respecto a la MS se observa un incremento en los distintos niveles de inclusión del carbonato de calcio a una temperatura de 20ºC durante la fermentación, aunque el mayor valor alcanzado de 39,66%. Los dos indicadores de proteína se observan diferencias en todos los niveles de inclusión de carbonato con respecto al tiempo y a las temperaturas estudiadas. Sin embargo, se mantienen los niveles de proteína bruta en comparación con los del preparado microbial obteniendo el valor más alto con la inclusión del 0,50% de CaCO3 a los 20ºC, presentando una diferencia marcada con los tenores de proteína a los 25ºC los cuales siempre estuvieron porcentualmente por debajo de la fermentación a los 20ºC. Se concluye que la mejor inclusión del CaCO3 en la fermentación de los residuales poscosecha de Solanum tuberosumcon el preparado microbial fue de 0,50% a los 20ºC, el cual permitió un crecimiento adecuado de los microorganismos BAL reflejado en el incremento de la proteína verdadera. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2017-06-13 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6145 10.17584/rcch.2017v11i1.6145 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No. 1 (2017); 143-150 Revista Colombiana de Ciencias Hortícolas; Vol. 11 Núm. 1 (2017); 143-150 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No 1 (2017); 143-150 Revista Colombiana de Ciencias Hortícolas; V. 11 N. 1 (2017); 143-150 Revista Colombiana de Ciencias Hortícolas; v. 11 n. 1 (2017); 143-150 2422-3719 2011-2173 spa https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6145/pdf
spellingShingle pH
Microbiological analysis
Lactic acid
Bromatology
Agriculture
pH
Análisis microbiológico
Ácido láctico
Bromatología
Agricultura
Borras, Luis Miguel
Rodríguez, Carlos Eduardo
Rodríguez, Ángela
Evaluation of calcium carbonate (CaCO3) inclusion in solid-state kinetic fermentation of Solanum tuberosum postharvest waste
title Evaluation of calcium carbonate (CaCO3) inclusion in solid-state kinetic fermentation of Solanum tuberosum postharvest waste
title_alt Inclusión de carbonato de calcio (CaCO3) en la cinética de fermentación en estado sólido de residuos de poscosecha de Solanum tuberosum
title_full Evaluation of calcium carbonate (CaCO3) inclusion in solid-state kinetic fermentation of Solanum tuberosum postharvest waste
title_fullStr Evaluation of calcium carbonate (CaCO3) inclusion in solid-state kinetic fermentation of Solanum tuberosum postharvest waste
title_full_unstemmed Evaluation of calcium carbonate (CaCO3) inclusion in solid-state kinetic fermentation of Solanum tuberosum postharvest waste
title_short Evaluation of calcium carbonate (CaCO3) inclusion in solid-state kinetic fermentation of Solanum tuberosum postharvest waste
title_sort evaluation of calcium carbonate caco3 inclusion in solid state kinetic fermentation of solanum tuberosum postharvest waste
topic pH
Microbiological analysis
Lactic acid
Bromatology
Agriculture
pH
Análisis microbiológico
Ácido láctico
Bromatología
Agricultura
topic_facet pH
Microbiological analysis
Lactic acid
Bromatology
Agriculture
pH
Análisis microbiológico
Ácido láctico
Bromatología
Agricultura
url https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6145
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