LED lights in the in vitro multiplication of three banana cultivars
Light emitting diodes (LEDs) are considered the most promising new source of light for the in vitro cultivation of plants because they have a long list of benefits and positively affect the growth of explants. The objective of this study was to evaluate the use of LEDs as an alternative light source...
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| Format: | Online |
| Language: | por |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2018
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6666 |
| _version_ | 1801706273098432512 |
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| author | Gomes da Rocha, Paulo Sérgio Pedroso de Oliveira, Roberto Bueno Scivittaro, Walkyria Mosele, Sergio Henrique |
| author_facet | Gomes da Rocha, Paulo Sérgio Pedroso de Oliveira, Roberto Bueno Scivittaro, Walkyria Mosele, Sergio Henrique |
| author_sort | Gomes da Rocha, Paulo Sérgio |
| collection | OJS |
| description | Light emitting diodes (LEDs) are considered the most promising new source of light for the in vitro cultivation of plants because they have a long list of benefits and positively affect the growth of explants. The objective of this study was to evaluate the use of LEDs as an alternative light source, using fluorescent lamps in the multiplication of three banana cultivars. The Grande Naine, FHIA 18 and Prata Anã cultivars were placed in an MS (Murashige and Skoog) medium with 30 g L-1 sucrose, 100 mg L-1 myo-inositol, 7 g L-1 agar and 6 mg L-1 BAP; three successive subcultures were carried out for 30 days under five light sources (blue-EDEB LEDs 3LA1 470 nm, green-TEDS LEDs 3LA1 530 nm, red-EDER LEDs 3LA3 630 nm, Growlux fluorescent lamps and white fluorescent lamps), with a 16 hour photoperiod, temperature of 25±2°C and light intensity of 20 µmol m-2 s-1. The design was completely randomized with a 5×3 factorial (light source × cultivar), with five repetitions. For all three cultivars, it was observed that the number of shoots and leaves was not influenced by the type of light. The highest number of formed shoots was obtained with the cultivar Grande Naine (2.6 buds/explant), with no difference between cv. FHIA 18 and Prata Anã (1.4 and 1.3 shoots/explant). There was a significant difference between the cultivars and light source for the length of budding; the longest length was obtained with Prata Anã, grown under green LEDs. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-6666 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | por |
| publishDate | 2018 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-66662020-08-03T23:58:16Z LED lights in the in vitro multiplication of three banana cultivars Uso de LEDs na multiplicação in vitro de três cultivares de bananeira Gomes da Rocha, Paulo Sérgio Pedroso de Oliveira, Roberto Bueno Scivittaro, Walkyria Mosele, Sergio Henrique Tissue culture light emitting diodes explant light quality Musa. SB317.5-319.864 SB354-402 SB119-124 Cultura de tecidos diodos emissores de luz explante qualidade da luz Musa. SB317.5-319.864 SB354-402 SB119-124 Light emitting diodes (LEDs) are considered the most promising new source of light for the in vitro cultivation of plants because they have a long list of benefits and positively affect the growth of explants. The objective of this study was to evaluate the use of LEDs as an alternative light source, using fluorescent lamps in the multiplication of three banana cultivars. The Grande Naine, FHIA 18 and Prata Anã cultivars were placed in an MS (Murashige and Skoog) medium with 30 g L-1 sucrose, 100 mg L-1 myo-inositol, 7 g L-1 agar and 6 mg L-1 BAP; three successive subcultures were carried out for 30 days under five light sources (blue-EDEB LEDs 3LA1 470 nm, green-TEDS LEDs 3LA1 530 nm, red-EDER LEDs 3LA3 630 nm, Growlux fluorescent lamps and white fluorescent lamps), with a 16 hour photoperiod, temperature of 25±2°C and light intensity of 20 µmol m-2 s-1. The design was completely randomized with a 5×3 factorial (light source × cultivar), with five repetitions. For all three cultivars, it was observed that the number of shoots and leaves was not influenced by the type of light. The highest number of formed shoots was obtained with the cultivar Grande Naine (2.6 buds/explant), with no difference between cv. FHIA 18 and Prata Anã (1.4 and 1.3 shoots/explant). There was a significant difference between the cultivars and light source for the length of budding; the longest length was obtained with Prata Anã, grown under green LEDs. Os diodos emissores de luz (LEDs) são considerados a mais nova e promissora fonte de luz para o cultivo in vitro de plantas, por apresentarem uma longa lista de benefícios e afetarem positivamente o crescimento dos explantes. O objetivo deste trabalho foi avaliar o uso dos LEDs como fonte de luz alternativa às lâmpadas fluorescentes na multiplicação de três cultivares de bananeira. A multiplicação das cultivares Grande Naine, FHIA 18 e Prata Anã foi realizada em meio MS (Murashige e Skoog) acrescido de 30 g L-1 de sacarose, 100 mg L-1 de mio-inositol, 7 g L-1 de ágar e 6 mg L-1 de BAP, por três subcultivos sucessivos de 30 dias, sob cinco fontes de luz (LEDs azuis-EDEB 3LA1 470 nm, LEDs verdes-EDET 3LA1 530 nm, LEDs vermelhos-EDER 3LA3 630 nm, lâmpadas fluorescentes Growlux e lâmpadas fluorescentes brancas), fotoperíodo de 16 horas, temperatura de 25±2ºC e intensidade luminosa de 20 µmol m-2 s-1. Utilizou-se delineamento inteiramente ao acaso, em esquema fatorial 5×3 (fonte de luz × cultivar), com cinco repetições. Para as três cultivares de bananeira multiplicadas, observou-se que o número de brotações e folhas não foi influenciado pelo tipo de luz. O maior número de brotações formado foi obtido com a cultivar Grande Naine (2,6 brotos/explante), não havendo diferença entre a cv. FHIA 18 e Prata Anã (1,4 e 1,3 brotos/explante). Determinou-se significância entre os fatores cultivar e fonte de luz para o comprimento da brotação, sendo o maior comprimento obtido com ‘Prata Anã’ cultivadas sob os LEDs verdes. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018-01-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Experimental Experimental application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6666 10.17584/rcch.2017v11i2.6666 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No. 2 (2017); 247-252 Revista Colombiana de Ciencias Hortícolas; Vol. 11 Núm. 2 (2017); 247-252 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No 2 (2017); 247-252 Revista Colombiana de Ciencias Hortícolas; V. 11 N. 2 (2017); 247-252 Revista Colombiana de Ciencias Hortícolas; v. 11 n. 2 (2017); 247-252 2422-3719 2011-2173 por https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6666/pdf |
| spellingShingle | Tissue culture light emitting diodes explant light quality Musa. SB317.5-319.864 SB354-402 SB119-124 Cultura de tecidos diodos emissores de luz explante qualidade da luz Musa. SB317.5-319.864 SB354-402 SB119-124 Gomes da Rocha, Paulo Sérgio Pedroso de Oliveira, Roberto Bueno Scivittaro, Walkyria Mosele, Sergio Henrique LED lights in the in vitro multiplication of three banana cultivars |
| title | LED lights in the in vitro multiplication of three banana cultivars |
| title_alt | Uso de LEDs na multiplicação in vitro de três cultivares de bananeira |
| title_full | LED lights in the in vitro multiplication of three banana cultivars |
| title_fullStr | LED lights in the in vitro multiplication of three banana cultivars |
| title_full_unstemmed | LED lights in the in vitro multiplication of three banana cultivars |
| title_short | LED lights in the in vitro multiplication of three banana cultivars |
| title_sort | led lights in the in vitro multiplication of three banana cultivars |
| topic | Tissue culture light emitting diodes explant light quality Musa. SB317.5-319.864 SB354-402 SB119-124 Cultura de tecidos diodos emissores de luz explante qualidade da luz Musa. SB317.5-319.864 SB354-402 SB119-124 |
| topic_facet | Tissue culture light emitting diodes explant light quality Musa. SB317.5-319.864 SB354-402 SB119-124 Cultura de tecidos diodos emissores de luz explante qualidade da luz Musa. SB317.5-319.864 SB354-402 SB119-124 |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/6666 |
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