Propagation and in vitro tuberization of two varieties of potato
This study was conducted in the Laboratorio de Cultivo de Tejidos Vegetales of the Universidad Pedagógica y Tecnológica de Colombia with the purpose of acquiring knowledge on the behavior under in vitro conditions as to evaluate a methodology for the micropropagation of plantlets a...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2018
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/7132 |
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| author | Araque Barrera, Eyda Johanna Bohórquez Quintero, María de los Angeles Pacheco Díaz, José Estiben Correa Mora, Lenny Yojana Urquijo Ruiz, Johan Sebastian Castañeda Garzón, Sandra Liliana Pacheco Maldonado, José Constantino |
| author_facet | Araque Barrera, Eyda Johanna Bohórquez Quintero, María de los Angeles Pacheco Díaz, José Estiben Correa Mora, Lenny Yojana Urquijo Ruiz, Johan Sebastian Castañeda Garzón, Sandra Liliana Pacheco Maldonado, José Constantino |
| author_sort | Araque Barrera, Eyda Johanna |
| collection | OJS |
| description | This study was conducted in the Laboratorio de Cultivo de Tejidos Vegetales of the Universidad Pedagógica
y Tecnológica de Colombia with the purpose of acquiring knowledge on the behavior under in vitro conditions as to evaluate a methodology for the micropropagation of plantlets and the production of microtubers from two varieties of potato ( Solanum tuberosum L. ssp andigena) Diacol Capiro and Parda Pastusa. The nodal segments provided by the ICA were disinfected with 10 % NaOCl, Then cultured for multiplication in MS medium and MS+NAA (0.02 mg/L). For the rooting process, NAA or IBA was added into the culture medium or applied in 30 seconds pulses. Subsequently, micropropagated uninodal segments were cultured in tuberization medium, showing an increase in production when supplemented with BA (2.5 mg/L) and with 8 % saccharose in the two varieties. The protocols developed in this study allow a large number of micropropagated plantlets and production of microtubers of Diacol Capiro and Parda Pastusa; the plantlets and microtubers developed can be cultured under controlled conditions for the production of pre-basic seeds. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-7132 |
| institution | Revista Ciencia en Desarrollo |
| language | spa |
| publishDate | 2018 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-71322022-06-15T17:04:56Z Propagation and in vitro tuberization of two varieties of potato Propagación y Tuberización In Vitro de dos variedades de Papa Araque Barrera, Eyda Johanna Bohórquez Quintero, María de los Angeles Pacheco Díaz, José Estiben Correa Mora, Lenny Yojana Urquijo Ruiz, Johan Sebastian Castañeda Garzón, Sandra Liliana Pacheco Maldonado, José Constantino Diacol Capiro microtuberización Parda Pastusa propagación Solanum tuberosum Diacol Capiro microtuberization Parda Pastusa propagation Solanum tuberosum This study was conducted in the Laboratorio de Cultivo de Tejidos Vegetales of the Universidad Pedagógica y Tecnológica de Colombia with the purpose of acquiring knowledge on the behavior under in vitro conditions as to evaluate a methodology for the micropropagation of plantlets and the production of microtubers from two varieties of potato ( Solanum tuberosum L. ssp andigena) Diacol Capiro and Parda Pastusa. The nodal segments provided by the ICA were disinfected with 10 % NaOCl, Then cultured for multiplication in MS medium and MS+NAA (0.02 mg/L). For the rooting process, NAA or IBA was added into the culture medium or applied in 30 seconds pulses. Subsequently, micropropagated uninodal segments were cultured in tuberization medium, showing an increase in production when supplemented with BA (2.5 mg/L) and with 8 % saccharose in the two varieties. The protocols developed in this study allow a large number of micropropagated plantlets and production of microtubers of Diacol Capiro and Parda Pastusa; the plantlets and microtubers developed can be cultured under controlled conditions for the production of pre-basic seeds. La investigación se realizó en el Laboratorio de Cultivo de Tejidos Vegetales de la Universidad Pedagógica y Tecnológica de Colombia con el propósito de conocer el comportamiento in vitro y evaluar una metodología para propagación de plántulas y producción de microtubérculos de dos variedades de papa (Solanum tuberosum L. ssp andígena) Diacol Capiro y Parda Pastusa, a partir de segmentos nodales obtenidos de tubérculos suministrados por el ICA, desinfectados con NaOCl al 10 %, multiplicados en medio MS y MS+ANA (0.02 mg L-1) y enraizados con ANA o AIB adicionados al medio de cultivo o aplicados en pulsos de 30 segundos. Posteriormente, segmentos uninodales micropropagados se cultivaron en medio para tuberización, observándose una mayor producción en MS suplementado con 2.5 mg L-1 de BA y 8 % de sacarosa en las dos variedades. Los protocolos desarrollados en el presente estudio permitieron la obtención masiva de plántulas y la producción de microtubérculos de Diacol Capiro y Parda Pastusa; las plántulas y microtubérculos desarrollados pueden ser cultivados bajo condiciones controladas para la producción de semilla pre-básica. Universidad Pedagógica y Tecnológica de Colombia 2018-02-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Investigación application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/7132 10.19053/01217488.v9.n1.2018.7132 Ciencia En Desarrollo; Vol. 9 No. 1 (2018): Vol 9, Núm. 1 (2018): Enero - Junio; 21-31 Ciencia en Desarrollo; Vol. 9 Núm. 1 (2018): Vol 9, Núm. 1 (2018): Enero - Junio; 21-31 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/7132/pdf Derechos de autor 2018 CIENCIA EN DESARROLLO |
| spellingShingle | Diacol Capiro microtuberización Parda Pastusa propagación Solanum tuberosum Diacol Capiro microtuberization Parda Pastusa propagation Solanum tuberosum Araque Barrera, Eyda Johanna Bohórquez Quintero, María de los Angeles Pacheco Díaz, José Estiben Correa Mora, Lenny Yojana Urquijo Ruiz, Johan Sebastian Castañeda Garzón, Sandra Liliana Pacheco Maldonado, José Constantino Propagation and in vitro tuberization of two varieties of potato |
| title | Propagation and in vitro tuberization of two varieties of potato |
| title_alt | Propagación y Tuberización In Vitro de dos variedades de Papa |
| title_full | Propagation and in vitro tuberization of two varieties of potato |
| title_fullStr | Propagation and in vitro tuberization of two varieties of potato |
| title_full_unstemmed | Propagation and in vitro tuberization of two varieties of potato |
| title_short | Propagation and in vitro tuberization of two varieties of potato |
| title_sort | propagation and in vitro tuberization of two varieties of potato |
| topic | Diacol Capiro microtuberización Parda Pastusa propagación Solanum tuberosum Diacol Capiro microtuberization Parda Pastusa propagation Solanum tuberosum |
| topic_facet | Diacol Capiro microtuberización Parda Pastusa propagación Solanum tuberosum Diacol Capiro microtuberization Parda Pastusa propagation Solanum tuberosum |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/7132 |
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