Genetic diversity of Moniliophthora roreri using Amplified Fragment Length Polymorphism (AFLPs)
Cacao cultivation is affected by the Moniliophthora roreri phytopathogen, which causes large production losses in Norte de Santander. This study shows the results obtained using the AFLPs of M. roreri. The kit for analysis system II was standardized and applied to 56 samples of M. roreri, isolated f...
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Format: | Online |
Language: | spa |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2018
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Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7342 |
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author | Suárez-Contreras, Liliana Yanet |
author_facet | Suárez-Contreras, Liliana Yanet |
author_sort | Suárez-Contreras, Liliana Yanet |
collection | OJS |
description | Cacao cultivation is affected by the Moniliophthora roreri phytopathogen, which causes large production losses in Norte de Santander. This study shows the results obtained using the AFLPs of M. roreri. The kit for analysis system II was standardized and applied to 56 samples of M. roreri, isolated from nine municipalities. The combination with the highest number of polymorphisms was E-ACC/M-CAA. With 90 bands and a smaller number of alleles (6), the E-ACG/M-CAA combination yielded 90 bands and a larger number of alleles (23); the E-AAC/M-CAA combination had 94 bands and alleles (12). The data analysis was carried out according to the dendrogram of similarity or genetic distances of Nei, obtaining five groups with a high variability between samples, from 6.5 to 58%. By combining the morphological and molecular information, the level of genetic diversity in Moniliophthora roreri was determined. |
format | Online |
id | oai:oai.revistas.uptc.edu.co:article-7342 |
institution | Revista Colombiana de Ciencias Hortícolas |
language | spa |
publishDate | 2018 |
publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
record_format | ojs |
spelling | oai:oai.revistas.uptc.edu.co:article-73422020-08-03T23:57:37Z Genetic diversity of Moniliophthora roreri using Amplified Fragment Length Polymorphism (AFLPs) Diversidad genética de Moniliophthora roreri mediante Polimorfismo de Longitud de Fragmentos Amplificados (AFLPs) Suárez-Contreras, Liliana Yanet Molecular marker genetic similarity moniliasis phytopathogen molecular characterization. SB317.5-319.864 SB183-317 SB621-795 Marcador molecular similaridad genética moniliasis fitopatógeno caracterización molecular. SB317.5-319.864 SB183-317 SB621-795 Cacao cultivation is affected by the Moniliophthora roreri phytopathogen, which causes large production losses in Norte de Santander. This study shows the results obtained using the AFLPs of M. roreri. The kit for analysis system II was standardized and applied to 56 samples of M. roreri, isolated from nine municipalities. The combination with the highest number of polymorphisms was E-ACC/M-CAA. With 90 bands and a smaller number of alleles (6), the E-ACG/M-CAA combination yielded 90 bands and a larger number of alleles (23); the E-AAC/M-CAA combination had 94 bands and alleles (12). The data analysis was carried out according to the dendrogram of similarity or genetic distances of Nei, obtaining five groups with a high variability between samples, from 6.5 to 58%. By combining the morphological and molecular information, the level of genetic diversity in Moniliophthora roreri was determined. El cultivo de cacao, está afectado por el fitopatógeno Moniliophthora roreri, que causa gran pérdida en la producción en Norte de Santander. Este estudio muestra los resultados obtenidos utilizando AFLPs de M. roreri. Con el kit para sistema de análisis II, estandarizado y aplicado a 56 muestras de M. roreri, aisladas de nueve municipios. La combinación que presentó mayor número de polimorfismos fue E-ACC/ M-CAA, con 100 bandas y un número menor de alelos (6), con la combinación E-ACG/ M-CAA se obtuvieron 90 bandas y un número de alelos mayor (23), y con la combinación E-AAC/ M-CAA se presentaron 94 bandas y alelos (12). El análisis de datos se realizó según el dendrograma de similitud o distancias genéticas de Nei, obteniéndose cinco grupos con una alta variabilidad entre las muestras, del 6,5 al 58%. Al combinar la información morfológica y molecular se determinó el nivel de diversidad genética de Moniliophthora roreri. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018-01-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Experimental Experimental application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7342 10.17584/rcch.2017v11i2.7342 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No. 2 (2017); 425-434 Revista Colombiana de Ciencias Hortícolas; Vol. 11 Núm. 2 (2017); 425-434 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No 2 (2017); 425-434 Revista Colombiana de Ciencias Hortícolas; V. 11 N. 2 (2017); 425-434 Revista Colombiana de Ciencias Hortícolas; v. 11 n. 2 (2017); 425-434 2422-3719 2011-2173 spa https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7342/pdf |
spellingShingle | Molecular marker genetic similarity moniliasis phytopathogen molecular characterization. SB317.5-319.864 SB183-317 SB621-795 Marcador molecular similaridad genética moniliasis fitopatógeno caracterización molecular. SB317.5-319.864 SB183-317 SB621-795 Suárez-Contreras, Liliana Yanet Genetic diversity of Moniliophthora roreri using Amplified Fragment Length Polymorphism (AFLPs) |
title | Genetic diversity of Moniliophthora roreri using Amplified Fragment Length Polymorphism (AFLPs) |
title_alt | Diversidad genética de Moniliophthora roreri mediante Polimorfismo de Longitud de Fragmentos Amplificados (AFLPs) |
title_full | Genetic diversity of Moniliophthora roreri using Amplified Fragment Length Polymorphism (AFLPs) |
title_fullStr | Genetic diversity of Moniliophthora roreri using Amplified Fragment Length Polymorphism (AFLPs) |
title_full_unstemmed | Genetic diversity of Moniliophthora roreri using Amplified Fragment Length Polymorphism (AFLPs) |
title_short | Genetic diversity of Moniliophthora roreri using Amplified Fragment Length Polymorphism (AFLPs) |
title_sort | genetic diversity of moniliophthora roreri using amplified fragment length polymorphism aflps |
topic | Molecular marker genetic similarity moniliasis phytopathogen molecular characterization. SB317.5-319.864 SB183-317 SB621-795 Marcador molecular similaridad genética moniliasis fitopatógeno caracterización molecular. SB317.5-319.864 SB183-317 SB621-795 |
topic_facet | Molecular marker genetic similarity moniliasis phytopathogen molecular characterization. SB317.5-319.864 SB183-317 SB621-795 Marcador molecular similaridad genética moniliasis fitopatógeno caracterización molecular. SB317.5-319.864 SB183-317 SB621-795 |
url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7342 |
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