Genetic diversity of Moringa oleifera Lam. in the northeast of Colombia using RAMs markers

Moringa oleifera Lam., also known as Moringa pterygosperma Gaertn., is a fast growing tree, indigenous to Himalaya that has been used as food or as medicine, recognized for its bioactive compounds. In Colombia, moringa has been promoted primarily because of its medicinal properties. However, despite...

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Main Authors:	Chaves-Bedoya, Giovanni, Galvis-Perez, Zaida Lorena, Ortiz-Rojas, Luz Yineth
Format:	Online
Language:	spa
Published:	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018
Subjects:	Germoplasm polymorphism genetic resources plant breeding. SB317.5-319.864 SB183-317 SB107-109 Germoplasma polimorfismo recursos genéticos mejoramiento vegetal.
Online Access:	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7343
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_version_	1801706275810050048
author	Chaves-Bedoya, Giovanni Galvis-Perez, Zaida Lorena Ortiz-Rojas, Luz Yineth
author_facet	Chaves-Bedoya, Giovanni Galvis-Perez, Zaida Lorena Ortiz-Rojas, Luz Yineth
author_sort	Chaves-Bedoya, Giovanni
collection	OJS
description	Moringa oleifera Lam., also known as Moringa pterygosperma Gaertn., is a fast growing tree, indigenous to Himalaya that has been used as food or as medicine, recognized for its bioactive compounds. In Colombia, moringa has been promoted primarily because of its medicinal properties. However, despite the importance of this phytogenetic resource, there are no studies on its genetic variability. In this study, we analyzed 45 accessions of moringa from four departments in the northeast of Colombia. The DICE and Nei-Li coefficients, at a level of similarity of 0.75, differentiated the population into four genetic groups. The expected heterozygosity was 0.13 for the oligo CT and 0.29 for the oligos CGA and GT. The percentage of polymorphic loci ranged from 31 to 100% for the primers CT and CA, respectively. With the UPGMA grouping, we revealed that the Moringa accessions from Villa del Rosario in Norte de Santander are genetically isolated. The genetic diversity of the studied Moringa accessions was low. This knowledge on the genetic variability of moringa provides a new contribution to Colombian growers that can be used in future breeding programs.
format	Online
id	oai:oai.revistas.uptc.edu.co:article-7343
institution	Revista Colombiana de Ciencias Hortícolas
language	spa
publishDate	2018
publisher	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
record_format	ojs
spelling	oai:oai.revistas.uptc.edu.co:article-73432020-08-03T23:57:41Z Genetic diversity of Moringa oleifera Lam. in the northeast of Colombia using RAMs markers Diversidad genética de Moringa oleifera Lam. en el nororiente colombiano utilizando marcadores RAMs Chaves-Bedoya, Giovanni Galvis-Perez, Zaida Lorena Ortiz-Rojas, Luz Yineth Germoplasm polymorphism genetic resources plant breeding. SB317.5-319.864 SB183-317 SB107-109 Germoplasma polimorfismo recursos genéticos mejoramiento vegetal. SB317.5-319.864 SB183-317 SB107-109 Moringa oleifera Lam., also known as Moringa pterygosperma Gaertn., is a fast growing tree, indigenous to Himalaya that has been used as food or as medicine, recognized for its bioactive compounds. In Colombia, moringa has been promoted primarily because of its medicinal properties. However, despite the importance of this phytogenetic resource, there are no studies on its genetic variability. In this study, we analyzed 45 accessions of moringa from four departments in the northeast of Colombia. The DICE and Nei-Li coefficients, at a level of similarity of 0.75, differentiated the population into four genetic groups. The expected heterozygosity was 0.13 for the oligo CT and 0.29 for the oligos CGA and GT. The percentage of polymorphic loci ranged from 31 to 100% for the primers CT and CA, respectively. With the UPGMA grouping, we revealed that the Moringa accessions from Villa del Rosario in Norte de Santander are genetically isolated. The genetic diversity of the studied Moringa accessions was low. This knowledge on the genetic variability of moringa provides a new contribution to Colombian growers that can be used in future breeding programs. Moringa oleifera, Lam., también conocida como Moringa pterygosperma Gaertn., es un árbol endógeno de la región del Himalaya, de crecimiento rápido que puede alcanzar los 12 m de alto. Moringa es empleado como alimento o como medicina, reconocido por sus compuestos bioactivos. En Colombia, moringa se ha promovido principalmente por sus propiedades medicinales. Sin embargo, a pesar de la importancia de este recurso fitogenético, no existen estudios de su variabilidad genética en el país. En esta investigación, empleando marcadores moleculares microsatelites amplificados al azar (RAMs), se analizaron 45 accesiones de moringa procedentes de 4 departamentos del Nor Oriente de Colombia. Los coeficientes de Dice y Nei-Li a un nivel de similitud de 0,75 diferenciaron la población en 4 grupos genéticos. La heterocigocidad estimada fue de 0,13 para el cebador CT y de 0,29 para los cebadores CGA y GT. El porcentaje de loci polimórfico osciló entre 31 y 100% para los cebadores CT y CA, respectivamente. Por la agrupación UPGMA, se identificó que las accesiones de Villa del Rosario en Norte de Santander están genéticamente aislados. Se encontró que la diversidad genética de las accesiones de Moringa estudiadas es baja. El conocimiento de la variabilidad genética de moringa en las regiones de estudio, proporciona un nuevo aporte a los cultivadores colombianos que puede ser empleado en futuros programas de mejoramiento. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018-01-06 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Experimental Experimental application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7343 10.17584/rcch.2017v11i2.7343 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No. 2 (2017); 408-415 Revista Colombiana de Ciencias Hortícolas; Vol. 11 Núm. 2 (2017); 408-415 Revista Colombiana de Ciencias Hortícolas; Vol. 11 No 2 (2017); 408-415 Revista Colombiana de Ciencias Hortícolas; V. 11 N. 2 (2017); 408-415 Revista Colombiana de Ciencias Hortícolas; v. 11 n. 2 (2017); 408-415 2422-3719 2011-2173 spa https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7343/pdf
spellingShingle	Germoplasm polymorphism genetic resources plant breeding. SB317.5-319.864 SB183-317 SB107-109 Germoplasma polimorfismo recursos genéticos mejoramiento vegetal. SB317.5-319.864 SB183-317 SB107-109 Chaves-Bedoya, Giovanni Galvis-Perez, Zaida Lorena Ortiz-Rojas, Luz Yineth Genetic diversity of Moringa oleifera Lam. in the northeast of Colombia using RAMs markers
title	Genetic diversity of Moringa oleifera Lam. in the northeast of Colombia using RAMs markers
title_alt	Diversidad genética de Moringa oleifera Lam. en el nororiente colombiano utilizando marcadores RAMs
title_full	Genetic diversity of Moringa oleifera Lam. in the northeast of Colombia using RAMs markers
title_fullStr	Genetic diversity of Moringa oleifera Lam. in the northeast of Colombia using RAMs markers
title_full_unstemmed	Genetic diversity of Moringa oleifera Lam. in the northeast of Colombia using RAMs markers
title_short	Genetic diversity of Moringa oleifera Lam. in the northeast of Colombia using RAMs markers
title_sort	genetic diversity of moringa oleifera lam in the northeast of colombia using rams markers
topic	Germoplasm polymorphism genetic resources plant breeding. SB317.5-319.864 SB183-317 SB107-109 Germoplasma polimorfismo recursos genéticos mejoramiento vegetal. SB317.5-319.864 SB183-317 SB107-109
topic_facet	Germoplasm polymorphism genetic resources plant breeding. SB317.5-319.864 SB183-317 SB107-109 Germoplasma polimorfismo recursos genéticos mejoramiento vegetal. SB317.5-319.864 SB183-317 SB107-109
url	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7343
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