Synthesis, antibacterial activity and interaction of DNA with lanthanide-β-cyclodextrin inclusion complexes
In this work, lanthanide complexes were synthesized starting from the corresponding La (III), Ce (III), Sm (III) and Yb (III) chlorides and cinnamate ligands which present bidentate coordination between the carboxyl group of the ligand and the lanthanide metal. These compounds were used as...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2018
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/7365 |
| _version_ | 1801706340837490688 |
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| author | Polo Cerón, Dorian Mayor-Rivera, Angélica María Aragón-Muriel, Alberto |
| author_facet | Polo Cerón, Dorian Mayor-Rivera, Angélica María Aragón-Muriel, Alberto |
| author_sort | Polo Cerón, Dorian |
| collection | OJS |
| description | In this work, lanthanide complexes were synthesized starting from the corresponding La (III), Ce (III), Sm (III) and Yb (III) chlorides and cinnamate ligands which present bidentate coordination between the carboxyl group of the ligand and the lanthanide metal. These compounds were used as hosts of β-cyclodextrin to obtain new inclusion complexes by a co-precipitation method using N,N-dimethylformamide as solvent. The inclusion products were characterized by IR-ATR spectroscopy, Raman, UV-vis, 1H and 13C NMR, XRD, TGA-DSC, elemental analysis and EDTA complexometry. Antibacterial activity tests were performed using six ATTC strains (S. aureus ATCC 25923, S. aureus ATCC 29213, E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. Typhimurium ATCC 14028 and
K. pneumoniae ATCC BAA-2146) by the microdilution method with Mueller-Hinton broth. The results of the biological activity for the lanthanide complexes showed the synergistic effect between the lanthanide cation and the cinnamate ligand. For the inclusion complexes, a decrease of the minimum inhibitory concentration (MIC) was observed with respect to the initial lanthanide complexes. The results obtained with the bovine thymus DNA and the plasmid pBR322 DNA allow to propose an electrostatic interaction between the evaluated complexes and the molécular structure of the DNA. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-7365 |
| institution | Revista Ciencia en Desarrollo |
| language | spa |
| publishDate | 2018 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-73652022-06-15T17:03:21Z Synthesis, antibacterial activity and interaction of DNA with lanthanide-β-cyclodextrin inclusion complexes Síntesis, actividad antibacteriana e interacción del ADN con complejos de inclusión entre compuestos lantánidos y β-ciclodextrina Polo Cerón, Dorian Mayor-Rivera, Angélica María Aragón-Muriel, Alberto actividad antibacteriana complejos de inclusión complejos lantánidos interacción con ADN antibacterial activity, inclusion complexes, interaction with DNA, lanthanide complexes In this work, lanthanide complexes were synthesized starting from the corresponding La (III), Ce (III), Sm (III) and Yb (III) chlorides and cinnamate ligands which present bidentate coordination between the carboxyl group of the ligand and the lanthanide metal. These compounds were used as hosts of β-cyclodextrin to obtain new inclusion complexes by a co-precipitation method using N,N-dimethylformamide as solvent. The inclusion products were characterized by IR-ATR spectroscopy, Raman, UV-vis, 1H and 13C NMR, XRD, TGA-DSC, elemental analysis and EDTA complexometry. Antibacterial activity tests were performed using six ATTC strains (S. aureus ATCC 25923, S. aureus ATCC 29213, E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. Typhimurium ATCC 14028 and K. pneumoniae ATCC BAA-2146) by the microdilution method with Mueller-Hinton broth. The results of the biological activity for the lanthanide complexes showed the synergistic effect between the lanthanide cation and the cinnamate ligand. For the inclusion complexes, a decrease of the minimum inhibitory concentration (MIC) was observed with respect to the initial lanthanide complexes. The results obtained with the bovine thymus DNA and the plasmid pBR322 DNA allow to propose an electrostatic interaction between the evaluated complexes and the molécular structure of the DNA. En este trabajo se han sintetizado complejos de lantánidos a partir de los derivados cloruros de La(III), Ce(III), Sm (III) e Yb(III) con ligandos cinamato, presentando coordinación bidentada entre el grupo carboxilo del ligando y el metal lantánido. Estos compuestos se utilizaron como huéspedes de la β-ciclodextrina con el fin de obtener complejos de inclusión mediante el método de co-precipitación, utilizando N,N-dimetilformamida como disolvente. Los productos de inclusión obtenidos fueron caracterizados mediante espectroscopia IR-ATR, Raman, UV-vis, RMN 1H, RMN 13C, DRX, TGA-DSC, análisis elemental y complexometría con EDTA. Se realizaron pruebas de actividad antibacteriana empleando 6 cepas ATTC (S. aureus ATCC 25923, S. aureus ATCC 29213, E. coli ATCC 25922, P. aeruginosa ATCC 27853, S. Typhimurium ATCC 14028 y K. pneumoniae ATCC BAA-2146) mediante el método de microdilución con caldo Mueller-Hinton; los resultados de actividad biológica para los complejos lantánidos permitieron evidenciar el efecto sinérgico entre el catión lantánido y el ligando cinamato. Igualmente, para los complejos de inclusión se observó una disminución de la concentración mínima inhibitoria (CMI) respecto a los complejos lantánidos iniciales. Los resultados obtenidos con el ADN de timo de ternera y el ADN plasmídico pBR322 permiten proponer una interacción electrostática entre los complejos evaluados y la estructura molecular del ADN. Universidad Pedagógica y Tecnológica de Colombia 2018-07-04 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/7365 10.19053/01217488.v9.n2.2018.7365 Ciencia En Desarrollo; Vol. 9 No. 2 (2018): Vol 9, Núm. 2 (2018): Vol 9, Núm 2(2018): Julio- Diciembre; 99-117 Ciencia en Desarrollo; Vol. 9 Núm. 2 (2018): Vol 9, Núm. 2 (2018): Vol 9, Núm 2(2018): Julio- Diciembre; 99-117 2462-7658 0121-7488 spa https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/7365/7263 Derechos de autor 2018 CIENCIA EN DESARROLLO |
| spellingShingle | actividad antibacteriana complejos de inclusión complejos lantánidos interacción con ADN antibacterial activity, inclusion complexes, interaction with DNA, lanthanide complexes Polo Cerón, Dorian Mayor-Rivera, Angélica María Aragón-Muriel, Alberto Synthesis, antibacterial activity and interaction of DNA with lanthanide-β-cyclodextrin inclusion complexes |
| title | Synthesis, antibacterial activity and interaction of DNA with lanthanide-β-cyclodextrin inclusion complexes |
| title_alt | Síntesis, actividad antibacteriana e interacción del ADN con complejos de inclusión entre compuestos lantánidos y β-ciclodextrina |
| title_full | Synthesis, antibacterial activity and interaction of DNA with lanthanide-β-cyclodextrin inclusion complexes |
| title_fullStr | Synthesis, antibacterial activity and interaction of DNA with lanthanide-β-cyclodextrin inclusion complexes |
| title_full_unstemmed | Synthesis, antibacterial activity and interaction of DNA with lanthanide-β-cyclodextrin inclusion complexes |
| title_short | Synthesis, antibacterial activity and interaction of DNA with lanthanide-β-cyclodextrin inclusion complexes |
| title_sort | synthesis antibacterial activity and interaction of dna with lanthanide β cyclodextrin inclusion complexes |
| topic | actividad antibacteriana complejos de inclusión complejos lantánidos interacción con ADN antibacterial activity, inclusion complexes, interaction with DNA, lanthanide complexes |
| topic_facet | actividad antibacteriana complejos de inclusión complejos lantánidos interacción con ADN antibacterial activity, inclusion complexes, interaction with DNA, lanthanide complexes |
| url | https://revistas.uptc.edu.co/index.php/ciencia_en_desarrollo/article/view/7365 |
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