Chlorophyll concentration estimation using non-destructive methods in grapes (Vitis vinifera L.) cv. Riesling Becker

Pigment analysis is a laboratory procedure and is not an immediate measurement to be performed in situ. The SPAD 502 and CCM-200 are equipments used for non-destructive, fast, and indirect estimates of chlorophyll contents. The concentration of photosynthetic pigments is related to the foliar concen...

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Main Authors: Castañeda, Cristian Santiago, Almanza-Merchán, Pedro José, Pinzón, Elberth Hernando, Cely, German Eduardo, Serrano, Pablo Antonio
Format: Online
Language:spa
Published: Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018
Subjects:
Online Access:https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7566
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author Castañeda, Cristian Santiago
Almanza-Merchán, Pedro José
Pinzón, Elberth Hernando
Cely, German Eduardo
Serrano, Pablo Antonio
author_facet Castañeda, Cristian Santiago
Almanza-Merchán, Pedro José
Pinzón, Elberth Hernando
Cely, German Eduardo
Serrano, Pablo Antonio
author_sort Castañeda, Cristian Santiago
collection OJS
description Pigment analysis is a laboratory procedure and is not an immediate measurement to be performed in situ. The SPAD 502 and CCM-200 are equipments used for non-destructive, fast, and indirect estimates of chlorophyll contents. The concentration of photosynthetic pigments is related to the foliar concentration of nitrogen, so indirectly, it is possible to observe the deficiency or excess of this element, providing a technical basis to suggest the proper management of the crop in order to potentiate the photosynthetic efficiency, quality and yield of a vineyard. This research sought to compare measurements between the portable chlorophyll meter SPAD-502 vs. CCM-200 in the Riesling Becker grape variety under cold tropical climate conditions to determine the behavior of chlorophyll during the growth of leaves 4, 5 and 6, from flowering to harvest. The relationship between the measurements with the SPAD-502 and the CCM-200 were fit to a second degree polynomial determined with the equation for the Chlorophyll Concentration Index: CCI = 0.014SPAD2-0.2396SPAD + 5.8021. With a value of R2=0.9343 (n=96; P≤0.0001), which indicates that there was a high correlation between the portable equipment used in the present study to determine chlorophyll with a non-destructive method. The evaluations carried out with the equipment obtained fast and reliable measurements, with the choice of the moment of measurement according to the phenological stage of the plant being important because of the high variability in chlorophyll contents that are presented in relation to the phylotaxy.
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spelling oai:oai.revistas.uptc.edu.co:article-75662020-08-03T23:57:22Z Chlorophyll concentration estimation using non-destructive methods in grapes (Vitis vinifera L.) cv. Riesling Becker Estimación de la concentración de clorofila mediante métodos no destructivos en vid (Vitis vinifera L.) cv. Riesling Becker Castañeda, Cristian Santiago Almanza-Merchán, Pedro José Pinzón, Elberth Hernando Cely, German Eduardo Serrano, Pablo Antonio Pigments Portable meter CCM-200 SPAD 502 Non-destructive methods Tropical vineyards Diagnostic indicators Fruits grapes Chlorophyll Pigmentos Medidor portátil CCM-200 SPAD 502 Métodos no destructivos Viñedos tropicales Indicadores diagnósticos Frutales uvas clorofila Pigments Compteur portable CCM-200 SPAD 502 Méthodes non destructives Vignobles tropicaux Indicateurs de diagnostic Pigmenti Misuratore portatile CCM-200 SPAD 502 Metodi non distruttivi Vigneti tropicali Indicatori diagnostici Pigmentos Medidor portátil CCM-200 SPAD 502 Métodos não destrutivos Vinhas tropicais Indicadores de diagnóstico Pigment analysis is a laboratory procedure and is not an immediate measurement to be performed in situ. The SPAD 502 and CCM-200 are equipments used for non-destructive, fast, and indirect estimates of chlorophyll contents. The concentration of photosynthetic pigments is related to the foliar concentration of nitrogen, so indirectly, it is possible to observe the deficiency or excess of this element, providing a technical basis to suggest the proper management of the crop in order to potentiate the photosynthetic efficiency, quality and yield of a vineyard. This research sought to compare measurements between the portable chlorophyll meter SPAD-502 vs. CCM-200 in the Riesling Becker grape variety under cold tropical climate conditions to determine the behavior of chlorophyll during the growth of leaves 4, 5 and 6, from flowering to harvest. The relationship between the measurements with the SPAD-502 and the CCM-200 were fit to a second degree polynomial determined with the equation for the Chlorophyll Concentration Index: CCI = 0.014SPAD2-0.2396SPAD + 5.8021. With a value of R2=0.9343 (n=96; P≤0.0001), which indicates that there was a high correlation between the portable equipment used in the present study to determine chlorophyll with a non-destructive method. The evaluations carried out with the equipment obtained fast and reliable measurements, with the choice of the moment of measurement according to the phenological stage of the plant being important because of the high variability in chlorophyll contents that are presented in relation to the phylotaxy. El análisis de pigmentos en plantas es un procedimiento de laboratorio y comúnmente no es una medida inmediata que se realice in situ. El SPAD 502 y CCM-200 son equipos para estimar de modo indirecto, no destructivo y rápido el contenido de clorofila. La concentración de pigmentos fotosintéticos se relaciona con la concentración foliar de nitrógeno, por lo que de forma indirecta se puede conocer la deficiencia o exceso del elemento, puede servir como fundamento técnico para sugerir el manejo adecuado del cultivo, en busca de potencializar la eficiencia fotosintética, calidad y el rendimiento de un viñedo. La investigación buscó comparar las mediciones entre el medidor portátil de clorofila SPAD-502 y CCM-200, en la variedad de uva Riesling Becker, bajo condiciones de clima frío tropical, y con ello determinar el comportamiento de clorofila durante el crecimiento de las hojas 4, 5 y 6, desde floración hasta vendimia. La relación entre las mediciones con el SPAD- 502 y el CCM-200 se ajustaron a un polinomio de segundo grado determinado por la ecuación, para el Índice de Concentración de Clorofila, como ICC = 0,014SPAD2-0,2396SPAD+5,8021. Con un valor de R2=0,9343 (n=96; P≤0,0001), el cual indica que existe una alta correlación entre los equipos portátiles empleados en el presente estudio para determinar la clorofila de forma no destructiva. Las evaluaciones realizadas con los equipos permitieron obtener medidas rápidas y confiables. Siendo importante la elección del momento de medición de acuerdo con el estadio fenológico de la planta debido a la alta variabilidad en los contenidos de clorofila que se presentan en relación a la filotaxia. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7566 10.17584/rcch.2018v12i2.7566 Revista Colombiana de Ciencias Hortícolas; Vol. 12 No. 2 (2018); 329-337 Revista Colombiana de Ciencias Hortícolas; Vol. 12 Núm. 2 (2018); 329-337 Revista Colombiana de Ciencias Hortícolas; Vol. 12 No 2 (2018); 329-337 Revista Colombiana de Ciencias Hortícolas; V. 12 N. 2 (2018); 329-337 Revista Colombiana de Ciencias Hortícolas; v. 12 n. 2 (2018); 329-337 2422-3719 2011-2173 spa https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7566/7067 Colombia-Boyaca; 2015-2017 Colombia-Boyaca; 2015-2017 Colombia-Boyaca; 2015-2017 Colombia-Boyaca; 2015-2017 Colombia-Boyaca; 2015-2017
spellingShingle Pigments
Portable meter
CCM-200
SPAD 502
Non-destructive methods
Tropical vineyards
Diagnostic indicators
Fruits
grapes
Chlorophyll
Pigmentos
Medidor portátil
CCM-200
SPAD 502
Métodos no destructivos
Viñedos tropicales
Indicadores diagnósticos
Frutales
uvas
clorofila
Pigments
Compteur portable
CCM-200
SPAD 502
Méthodes non destructives
Vignobles tropicaux
Indicateurs de diagnostic
Pigmenti
Misuratore portatile
CCM-200
SPAD 502
Metodi non distruttivi
Vigneti tropicali
Indicatori diagnostici
Pigmentos
Medidor portátil
CCM-200
SPAD 502
Métodos não destrutivos
Vinhas tropicais
Indicadores de diagnóstico
Castañeda, Cristian Santiago
Almanza-Merchán, Pedro José
Pinzón, Elberth Hernando
Cely, German Eduardo
Serrano, Pablo Antonio
Chlorophyll concentration estimation using non-destructive methods in grapes (Vitis vinifera L.) cv. Riesling Becker
title Chlorophyll concentration estimation using non-destructive methods in grapes (Vitis vinifera L.) cv. Riesling Becker
title_alt Estimación de la concentración de clorofila mediante métodos no destructivos en vid (Vitis vinifera L.) cv. Riesling Becker
title_full Chlorophyll concentration estimation using non-destructive methods in grapes (Vitis vinifera L.) cv. Riesling Becker
title_fullStr Chlorophyll concentration estimation using non-destructive methods in grapes (Vitis vinifera L.) cv. Riesling Becker
title_full_unstemmed Chlorophyll concentration estimation using non-destructive methods in grapes (Vitis vinifera L.) cv. Riesling Becker
title_short Chlorophyll concentration estimation using non-destructive methods in grapes (Vitis vinifera L.) cv. Riesling Becker
title_sort chlorophyll concentration estimation using non destructive methods in grapes vitis vinifera l cv riesling becker
topic Pigments
Portable meter
CCM-200
SPAD 502
Non-destructive methods
Tropical vineyards
Diagnostic indicators
Fruits
grapes
Chlorophyll
Pigmentos
Medidor portátil
CCM-200
SPAD 502
Métodos no destructivos
Viñedos tropicales
Indicadores diagnósticos
Frutales
uvas
clorofila
Pigments
Compteur portable
CCM-200
SPAD 502
Méthodes non destructives
Vignobles tropicaux
Indicateurs de diagnostic
Pigmenti
Misuratore portatile
CCM-200
SPAD 502
Metodi non distruttivi
Vigneti tropicali
Indicatori diagnostici
Pigmentos
Medidor portátil
CCM-200
SPAD 502
Métodos não destrutivos
Vinhas tropicais
Indicadores de diagnóstico
topic_facet Pigments
Portable meter
CCM-200
SPAD 502
Non-destructive methods
Tropical vineyards
Diagnostic indicators
Fruits
grapes
Chlorophyll
Pigmentos
Medidor portátil
CCM-200
SPAD 502
Métodos no destructivos
Viñedos tropicales
Indicadores diagnósticos
Frutales
uvas
clorofila
Pigments
Compteur portable
CCM-200
SPAD 502
Méthodes non destructives
Vignobles tropicaux
Indicateurs de diagnostic
Pigmenti
Misuratore portatile
CCM-200
SPAD 502
Metodi non distruttivi
Vigneti tropicali
Indicatori diagnostici
Pigmentos
Medidor portátil
CCM-200
SPAD 502
Métodos não destrutivos
Vinhas tropicais
Indicadores de diagnóstico
url https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7566
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