Identification and molecular characterization of the complete genome of three viruses infecting lulo (Solanum quitoense) crops in Antioquia (Colombia)
Lulo is one the most promising crops in the Andean region of Colombia because of its unique organoleptic properties and potential for industrial processing. In Antioquia, there has been a marked increase of lulo plants exhibiting typical symptoms of viral diseases, such as interveinal yellowing, mos...
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| Format: | Online |
| Language: | spa |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2018
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7692 |
| _version_ | 1801706280155348992 |
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| author | Gallo, Yuliana Toro, Luisa Fernanda Jaramillo, Helena Gutiérrez, Pablo Andrés Marín, Mauricio |
| author_facet | Gallo, Yuliana Toro, Luisa Fernanda Jaramillo, Helena Gutiérrez, Pablo Andrés Marín, Mauricio |
| author_sort | Gallo, Yuliana |
| collection | OJS |
| description | Lulo is one the most promising crops in the Andean region of Colombia because of its unique organoleptic properties and potential for industrial processing. In Antioquia, there has been a marked increase of lulo plants exhibiting typical symptoms of viral diseases, such as interveinal yellowing, mosaics and sprout deformation. In this research, we studied the presence of viruses infecting lulo in bulked leaf samples from Eastern Antioquia. Viruses were detected using Next-generation sequencing (NGS), with confirmation by RT-PCR. The NGS resulted in a total of 10’777,822 paired-end reads, of which 40.4% corresponded to Cucumber mosaic virus (CMV), 0.09% to Potato yellow vein virus (PYVV) and 0.06% to Alstroemeria necrotic streak virus (ANSV). Complete genome sequences were obtained for each of these viruses. RT-PCR primers for CMV and ANSV detection were designed using the assembled genomes reported in this study. Future research should investigate the effect of these viruses on crop yield, plant longevity and seed quality as well as their means of transmission, host range and specific symptoms. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-7692 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | spa |
| publishDate | 2018 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-76922020-08-03T23:57:20Z Identification and molecular characterization of the complete genome of three viruses infecting lulo (Solanum quitoense) crops in Antioquia (Colombia) Identificación y caracterización molecular del genoma completo de tres virus en cultivos de lulo (Solanum quitoense) de Antioquia (Colombia) Gallo, Yuliana Toro, Luisa Fernanda Jaramillo, Helena Gutiérrez, Pablo Andrés Marín, Mauricio Crinivirus Cucumovirus RT-PCR Next-generation sequencing Tospovirus Fruits lulo virus Crinivirus Cucumovirus RT-PCR Secuenciación de nueva generación Tospovirus Frutales lulo virus Crinivirus Cucumovirus RT-PCR Séquençage nouvelle génération Tospovirus arbres fruitiers lulo virus Crinivirus Cucumovirus RT-PCR Sequenziamento di prossima generazione Tospovirus alberi da frutto Lulo virus Crinivírus Cucumovirus RT-PCR Sequenciamento de próxima geração Tospovírus árvores frutíferas lulo vírus Lulo is one the most promising crops in the Andean region of Colombia because of its unique organoleptic properties and potential for industrial processing. In Antioquia, there has been a marked increase of lulo plants exhibiting typical symptoms of viral diseases, such as interveinal yellowing, mosaics and sprout deformation. In this research, we studied the presence of viruses infecting lulo in bulked leaf samples from Eastern Antioquia. Viruses were detected using Next-generation sequencing (NGS), with confirmation by RT-PCR. The NGS resulted in a total of 10’777,822 paired-end reads, of which 40.4% corresponded to Cucumber mosaic virus (CMV), 0.09% to Potato yellow vein virus (PYVV) and 0.06% to Alstroemeria necrotic streak virus (ANSV). Complete genome sequences were obtained for each of these viruses. RT-PCR primers for CMV and ANSV detection were designed using the assembled genomes reported in this study. Future research should investigate the effect of these viruses on crop yield, plant longevity and seed quality as well as their means of transmission, host range and specific symptoms. El lulo es uno de los renglones agrícolas más promisorios para la región andina de Colombia, gracias a sus excelentes características organolépticas y a su potencial para el procesamiento industrial. En Antioquia, se ha observado en los últimos años la presencia de plantas con síntomas de enfermedades virales, que incluyen amarillamientos intervenales, mosaicos y deformación de brotes. En este trabajo, se evaluó dicha situación en un grupo (bulk) de muestras foliares de plantas sintomáticas de lulo obtenidas en municipios del Oriente Antioqueño, utilizando la metodología molecular de secuenciación de nueva generación (NGS), y la confirmación posterior por RT-PCR. Los análisis bioinformáticos de las secuencias obtenidas (10.777.822 de reads) indicaron la presencia de tres virus de RNA en el transcriptoma evaluado; el Cucumber mosaic virus (CMV) se encontró en mayores niveles de infección (40,4% del total de reads), mientras que los otros virus correspondieron al Potato yellow vein virus (PYVV) (0,09%) y Alstroemeria necrotic streak virus (ANSV) (0,06%), siendo posible obtener sus genomas completos. La ocurrencia de los tres virus fue confirmada en plantas individuales de lulo mediante pruebas de RT-PCR/secuenciación Sanger con cebadores específicos diseñados a partir de los datos de NGS (ANSV y CMV), o con cebadores reportados previamente (PYVV). En el futuro será necesario evaluar los efectos de estos virus sobre los rendimientos, longevidad de plantas y calidad de semilla en los cultivos de lulo en el país, así como sus métodos de transmisión, rango de hospedantes y sintomatología específica. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto Texte Testo Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7692 10.17584/rcch.2018v12i2.7692 Revista Colombiana de Ciencias Hortícolas; Vol. 12 No. 2 (2018); 281-292 Revista Colombiana de Ciencias Hortícolas; Vol. 12 Núm. 2 (2018); 281-292 Revista Colombiana de Ciencias Hortícolas; Vol. 12 No 2 (2018); 281-292 Revista Colombiana de Ciencias Hortícolas; V. 12 N. 2 (2018); 281-292 Revista Colombiana de Ciencias Hortícolas; v. 12 n. 2 (2018); 281-292 2422-3719 2011-2173 spa https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7692/7045 Colombia; Antioquia; municipios: Marinilla, Sonsón y San Vicente Ferrer; Abril a Junio de 2017 Colombia; Antioquia; municipios: Marinilla, Sonsón y San Vicente Ferrer; Abril a Junio de 2017 Colombia; Antioquia; municipios: Marinilla, Sonsón y San Vicente Ferrer; Abril a Junio de 2017 Colombia; Antioquia; municipios: Marinilla, Sonsón y San Vicente Ferrer; Abril a Junio de 2017 Colombia; Antioquia; municipios: Marinilla, Sonsón y San Vicente Ferrer; Abril a Junio de 2017 |
| spellingShingle | Crinivirus Cucumovirus RT-PCR Next-generation sequencing Tospovirus Fruits lulo virus Crinivirus Cucumovirus RT-PCR Secuenciación de nueva generación Tospovirus Frutales lulo virus Crinivirus Cucumovirus RT-PCR Séquençage nouvelle génération Tospovirus arbres fruitiers lulo virus Crinivirus Cucumovirus RT-PCR Sequenziamento di prossima generazione Tospovirus alberi da frutto Lulo virus Crinivírus Cucumovirus RT-PCR Sequenciamento de próxima geração Tospovírus árvores frutíferas lulo vírus Gallo, Yuliana Toro, Luisa Fernanda Jaramillo, Helena Gutiérrez, Pablo Andrés Marín, Mauricio Identification and molecular characterization of the complete genome of three viruses infecting lulo (Solanum quitoense) crops in Antioquia (Colombia) |
| title | Identification and molecular characterization of the complete genome of three viruses infecting lulo (Solanum quitoense) crops in Antioquia (Colombia) |
| title_alt | Identificación y caracterización molecular del genoma completo de tres virus en cultivos de lulo (Solanum quitoense) de Antioquia (Colombia) |
| title_full | Identification and molecular characterization of the complete genome of three viruses infecting lulo (Solanum quitoense) crops in Antioquia (Colombia) |
| title_fullStr | Identification and molecular characterization of the complete genome of three viruses infecting lulo (Solanum quitoense) crops in Antioquia (Colombia) |
| title_full_unstemmed | Identification and molecular characterization of the complete genome of three viruses infecting lulo (Solanum quitoense) crops in Antioquia (Colombia) |
| title_short | Identification and molecular characterization of the complete genome of three viruses infecting lulo (Solanum quitoense) crops in Antioquia (Colombia) |
| title_sort | identification and molecular characterization of the complete genome of three viruses infecting lulo solanum quitoense crops in antioquia colombia |
| topic | Crinivirus Cucumovirus RT-PCR Next-generation sequencing Tospovirus Fruits lulo virus Crinivirus Cucumovirus RT-PCR Secuenciación de nueva generación Tospovirus Frutales lulo virus Crinivirus Cucumovirus RT-PCR Séquençage nouvelle génération Tospovirus arbres fruitiers lulo virus Crinivirus Cucumovirus RT-PCR Sequenziamento di prossima generazione Tospovirus alberi da frutto Lulo virus Crinivírus Cucumovirus RT-PCR Sequenciamento de próxima geração Tospovírus árvores frutíferas lulo vírus |
| topic_facet | Crinivirus Cucumovirus RT-PCR Next-generation sequencing Tospovirus Fruits lulo virus Crinivirus Cucumovirus RT-PCR Secuenciación de nueva generación Tospovirus Frutales lulo virus Crinivirus Cucumovirus RT-PCR Séquençage nouvelle génération Tospovirus arbres fruitiers lulo virus Crinivirus Cucumovirus RT-PCR Sequenziamento di prossima generazione Tospovirus alberi da frutto Lulo virus Crinivírus Cucumovirus RT-PCR Sequenciamento de próxima geração Tospovírus árvores frutíferas lulo vírus |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7692 |
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