Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L.
This study aimed at isolating diazotrophic bacteria with promoting activity of vegetal growth, associated to carrot culture. Samples were taken at 30, 60 and 115 days in a farm located in a rural area of the municipality of Manizales (Caldas). The diazotrophic bacteria were isolated in semi-solid cu...
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2018
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7753 |
| _version_ | 1801706472954920960 |
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| author | Gaviria-Giraldo, Jennifer Restrepo-Franco, Gloria María Galeano-Vanegas, Narmer Fernando Hernández-Rodríguez, Annia |
| author_facet | Gaviria-Giraldo, Jennifer Restrepo-Franco, Gloria María Galeano-Vanegas, Narmer Fernando Hernández-Rodríguez, Annia |
| author_sort | Gaviria-Giraldo, Jennifer |
| collection | OJS |
| description | This study aimed at isolating diazotrophic bacteria with promoting activity of vegetal growth, associated to carrot culture. Samples were taken at 30, 60 and 115 days in a farm located in a rural area of the municipality of Manizales (Caldas). The diazotrophic bacteria were isolated in semi-solid culture media free of nitrogen. We described the macro and microscopic features of the obtained isolations, and identified them biochemically and molecularly. In addition, we evaluated indole compounds, nitrogenase activity and solubilization of phosphates. Gluconacetobacter diazotrophicus ATCC 49037 was used as a standard strain. We recovered 20 isolates associated to rhizosphere and 12 to rhizoplane. The molecular identification showed the presence of five genera: Rhizobium, Achromobacter, Bacillus, Enterobacter, and Stenotrophomonas. The production of indole compounds presented concentrations between 9.73 and 112.8 μg/mL. The standard strain showed a higher activity, with an indolic compound production of 172.5 μg/mL. Regarding nitrogenase activity, the isolates GIBI411, 394 and 399 had an activity greater or similar to the standard strain. The most efficient isolates in the solubilization of tricalcium phosphate were GIBI378 and 385. The solubilization of aluminum phosphate was assessed by the acid production index, with the isolates GIBI378, 391, 387 and 388 presenting the best behavior. The isolates found are potential candidates for the development of novel biotechnological processes to produce new alternative biofertilizers, considering the significant plant-growth promotion properties determined in this work. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-7753 |
| institution | Revista Ciencia y Agricultura |
| language | spa |
| publishDate | 2018 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-77532022-06-16T15:19:56Z Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L. Bacterias diazotróficas con actividad promotora del crecimiento vegetal en Daucus carota L. Gaviria-Giraldo, Jennifer Restrepo-Franco, Gloria María Galeano-Vanegas, Narmer Fernando Hernández-Rodríguez, Annia carrot nitrogen fixation nitrogenase activity phosphate solubilization actividad nitrogenasa fijación de nitrógeno solubilización de fosfato zanahoria This study aimed at isolating diazotrophic bacteria with promoting activity of vegetal growth, associated to carrot culture. Samples were taken at 30, 60 and 115 days in a farm located in a rural area of the municipality of Manizales (Caldas). The diazotrophic bacteria were isolated in semi-solid culture media free of nitrogen. We described the macro and microscopic features of the obtained isolations, and identified them biochemically and molecularly. In addition, we evaluated indole compounds, nitrogenase activity and solubilization of phosphates. Gluconacetobacter diazotrophicus ATCC 49037 was used as a standard strain. We recovered 20 isolates associated to rhizosphere and 12 to rhizoplane. The molecular identification showed the presence of five genera: Rhizobium, Achromobacter, Bacillus, Enterobacter, and Stenotrophomonas. The production of indole compounds presented concentrations between 9.73 and 112.8 μg/mL. The standard strain showed a higher activity, with an indolic compound production of 172.5 μg/mL. Regarding nitrogenase activity, the isolates GIBI411, 394 and 399 had an activity greater or similar to the standard strain. The most efficient isolates in the solubilization of tricalcium phosphate were GIBI378 and 385. The solubilization of aluminum phosphate was assessed by the acid production index, with the isolates GIBI378, 391, 387 and 388 presenting the best behavior. The isolates found are potential candidates for the development of novel biotechnological processes to produce new alternative biofertilizers, considering the significant plant-growth promotion properties determined in this work. Este trabajo se propuso aislar bacterias diazotróficas con actividad promotora del crecimiento vegetal, asociadas al cultivo de zanahoria. Se realizaron 3 muestreos, a los 30, 60 y 115 días, en una granja ubicada en una zona rural del municipio de Manizales (Caldas). El aislamiento de las bacterias diazotróficas se efectuó en medios de cultivo semisólidos libres de nitrógeno. A los aislamientos obtenidos se les realizó descripción macro y microscópica, identificación bioquímica y molecular. Se evaluaron características como determinación de compuestos indólicos, actividad nitrogenasa y solubilización de fosfatos. Como cepa patrón se empleó Gluconacetobacter diazotrophicus ATCC 49037. Se recuperaron 20 aislamientos asociados a la rizosfera y 12 al rizoplano. La identificación molecular mostró cinco géneros presentes: Rhizobium, Achromobacter, Bacillus, Enterobacter y Stenotrophomonas. La producción de compuestos indólicos presentó concentraciones entre 9,73 y 112,8 µg/mL. La cepa patrón presentó una actividad mayor, con una producción de compuestos indólicos de 172,5 µg/mL. En la actividad nitrogenasa los aislamientos GIBI411, 394 y 399 tuvieron una actividad mayor o similar a la cepa patrón. Los aislamientos más eficientes en la solubilización de fosfato tricálcico fueron GIBI378 y 385. La solubilización de fosfato de aluminio se valoró por el índice de producción de ácido, siendo los aislamientos GIBI378, 391, 387 y 388 los de mejor comportamiento en esta variable. Los aislamientos encontrados son candidatos potenciales para desarrollar nuevos procesos biotecnológicos para la producción de nuevos biofertilizantes alternativos, considerando las importantes propiedades de promoción del crecimiento vegetal determinadas en este trabajo. Universidad Pedagógica y Tecnológica de Colombia 2018-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion research investigación application/pdf https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7753 10.19053/01228420.v15.n1.2018.7753 Ciencia y Agricultura; Vol. 15 No. 1 (2018); 19-27 Ciencia y Agricultura; Vol. 15 Núm. 1 (2018); 19-27 2539-0899 spa https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7753/6235 |
| spellingShingle | carrot nitrogen fixation nitrogenase activity phosphate solubilization actividad nitrogenasa fijación de nitrógeno solubilización de fosfato zanahoria Gaviria-Giraldo, Jennifer Restrepo-Franco, Gloria María Galeano-Vanegas, Narmer Fernando Hernández-Rodríguez, Annia Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L. |
| title | Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L. |
| title_alt | Bacterias diazotróficas con actividad promotora del crecimiento vegetal en Daucus carota L. |
| title_full | Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L. |
| title_fullStr | Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L. |
| title_full_unstemmed | Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L. |
| title_short | Diazotrophic bacteria with activity promoting vegetable growth in Daucus carota L. |
| title_sort | diazotrophic bacteria with activity promoting vegetable growth in daucus carota l |
| topic | carrot nitrogen fixation nitrogenase activity phosphate solubilization actividad nitrogenasa fijación de nitrógeno solubilización de fosfato zanahoria |
| topic_facet | carrot nitrogen fixation nitrogenase activity phosphate solubilization actividad nitrogenasa fijación de nitrógeno solubilización de fosfato zanahoria |
| url | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7753 |
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