In vitro effect of the hydroalcoholic and ethanolic extracts of papaya seeds (Carica papaya) against Haemonchus contortus
Among the main health problems for sheep production are gastrointestinal parasites, of which the most important nematode is Haemonchus contortus. Current control strategies focus on the effectiveness, along with the low impact on the ecosystem. The study of the integral control of parasites allows t...
| Main Authors: | , , , , , , |
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| Format: | Online |
| Language: | spa |
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Universidad Pedagógica y Tecnológica de Colombia
2018
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| Subjects: | |
| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7756 |
| _version_ | 1801706473338699776 |
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| author | Marroquín-Tun, María de los Ángeles Higuera-Piedrahita, Rosa Isabel López-Arellano, María Eugenia López-Arellano, Raquel de la Cruz-Cruz, Héctor Alejandro Silva-Mendoza, Rocío Cuéllar-Ordaz, Jorge Alfredo |
| author_facet | Marroquín-Tun, María de los Ángeles Higuera-Piedrahita, Rosa Isabel López-Arellano, María Eugenia López-Arellano, Raquel de la Cruz-Cruz, Héctor Alejandro Silva-Mendoza, Rocío Cuéllar-Ordaz, Jorge Alfredo |
| author_sort | Marroquín-Tun, María de los Ángeles |
| collection | OJS |
| description | Among the main health problems for sheep production are gastrointestinal parasites, of which the most important nematode is Haemonchus contortus. Current control strategies focus on the effectiveness, along with the low impact on the ecosystem. The study of the integral control of parasites allows the investigation of alternatives with probable anthelmintic effect, such as the use of papaya (Carica papaya) seeds. This study aimed at evaluating the in vitro effect of hydroalcoholic and ethanolic extracts of papaya seeds on eggs and larvae of H. contortus. The study was carried out with the equipment and facilities of Laboratory 3 of the Multidisciplinary Research Unit at the Facultad de Estudios Superiores Cuautitlán, UNAM. The extracts were prepared through maceration in solvent. A sheep was inoculated with 5000 L3 of H. contortus, a strain isolated and maintained in the facilities. Fecal samples were collected, and eggs were recovered for the hatch inhibition assay. The eggs and L3 were pretreated with hydroalcoholic and ethanolic extract of papaya seed (2.5 mg/ml), distilled water, ethanol 90 %, and levamisole (7.5 mg/ml). The abomasal explants technique was used to evaluate the effect of the extracts on L3 and its association to the abomasal mucosa. An ANOVA was performed for repeated samples using the Statgraphics program. The ethanolic extract of the papaya seed inhibited 92 % of hatching, and the hydroalcoholic extract 50 % (p <0.05). In the case of the explants, the ethanolic extract showed 10 % of the larvae not associated with the abomasal tissue, followed by the hydroalcoholic extract with 60 % of non-associated larvae (p<0.05). In conclusion, the ethanolic extract has an effect on eggs, while the hydroalcoholic extract inhibits the association to the abomasal tissue. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-7756 |
| institution | Revista Ciencia y Agricultura |
| language | spa |
| publishDate | 2018 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-77562022-06-16T15:19:50Z In vitro effect of the hydroalcoholic and ethanolic extracts of papaya seeds (Carica papaya) against Haemonchus contortus Efecto in vitro de los extractos hidroalcohólico y etanólico de semilla de papaya (Carica papaya) en Haemonchus contortus Marroquín-Tun, María de los Ángeles Higuera-Piedrahita, Rosa Isabel López-Arellano, María Eugenia López-Arellano, Raquel de la Cruz-Cruz, Héctor Alejandro Silva-Mendoza, Rocío Cuéllar-Ordaz, Jorge Alfredo Carica papaya ethanolic extract hydro alcoholic extract Haemonchus contortus seeds Carica papaya extracto etanólico extracto hidroalcohólico Haemonchus contortus Among the main health problems for sheep production are gastrointestinal parasites, of which the most important nematode is Haemonchus contortus. Current control strategies focus on the effectiveness, along with the low impact on the ecosystem. The study of the integral control of parasites allows the investigation of alternatives with probable anthelmintic effect, such as the use of papaya (Carica papaya) seeds. This study aimed at evaluating the in vitro effect of hydroalcoholic and ethanolic extracts of papaya seeds on eggs and larvae of H. contortus. The study was carried out with the equipment and facilities of Laboratory 3 of the Multidisciplinary Research Unit at the Facultad de Estudios Superiores Cuautitlán, UNAM. The extracts were prepared through maceration in solvent. A sheep was inoculated with 5000 L3 of H. contortus, a strain isolated and maintained in the facilities. Fecal samples were collected, and eggs were recovered for the hatch inhibition assay. The eggs and L3 were pretreated with hydroalcoholic and ethanolic extract of papaya seed (2.5 mg/ml), distilled water, ethanol 90 %, and levamisole (7.5 mg/ml). The abomasal explants technique was used to evaluate the effect of the extracts on L3 and its association to the abomasal mucosa. An ANOVA was performed for repeated samples using the Statgraphics program. The ethanolic extract of the papaya seed inhibited 92 % of hatching, and the hydroalcoholic extract 50 % (p <0.05). In the case of the explants, the ethanolic extract showed 10 % of the larvae not associated with the abomasal tissue, followed by the hydroalcoholic extract with 60 % of non-associated larvae (p<0.05). In conclusion, the ethanolic extract has an effect on eggs, while the hydroalcoholic extract inhibits the association to the abomasal tissue. Entre los principales problemas sanitarios para la producción ovina están los parásitos gastroentéricos; de ellos, el más importante es Haemonchus contortus. Las estrategias actuales de control enfocan la eficacia, junto con el bajo o nulo impacto al ecosistema; el estudio del control integral de parásitos permite la investigación de alternativas con probable efecto antihelmíntico, entre ellas el uso de semilla de papaya (Carica papaya). El objetivo de este trabajo fue evaluar el efecto in vitro de los extractos hidroalcohólico y etanólico de la semilla de papaya (C. papaya) sobre huevos y larvas de H. contortus. El trabajo se llevó a cabo con el equipo e instalaciones del Laboratorio 3 de la Unidad de Investigación Multidisciplinaria en la Facultad de Estudios Superiores Cuautitlán, UNAM. Se elaboraron los extractos por medio de maceración en solvente. Se inoculó un ovino con 5000 larvas de tercer estadio (L3) de H. contortus, cepa aislada y mantenida en dichas instalaciones. Se tomaron muestras de materia fecal y se recuperaron huevos para el ensayo de inhibición de la eclosión. Los huevos y las L3 fueron pretratados con extracto hidroalcohólico y etanólico de semilla de papaya (2.5 mg/ml), agua destilada, etanol 90 % y levamisol (7.5 mg/ml). Se efectuó la técnica de explantes abomasales, evaluando el efecto de los extractos sobre las L3 y su asociación a la mucosa abomasal. Se realizó un ANOVA para muestras repetidas por medio del programa Statgraphics. El extracto etanólico de semilla de papaya mostró el 92 % de inhibición de la eclosión; el extracto hidroalcohólico mostró 50 % (p<0.05). En el caso de los explantes, el extracto etanólico mostró el 10 % de larvas no asociadas al tejido abomasal, seguido por el extracto hidroalcohólico, con el 60 % de larvas no asociadas (p<0.05). Se concluye que el extracto etanólico tiene efecto sobre huevos, mientras que el extracto hidroalcohólico inhibe la asociación al tejido abomasal. Universidad Pedagógica y Tecnológica de Colombia 2018-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion research investigación application/pdf https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7756 10.19053/01228420.v15.n1.2018.7756 Ciencia y Agricultura; Vol. 15 No. 1 (2018); 53-59 Ciencia y Agricultura; Vol. 15 Núm. 1 (2018); 53-59 2539-0899 spa https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7756/6238 |
| spellingShingle | Carica papaya ethanolic extract hydro alcoholic extract Haemonchus contortus seeds Carica papaya extracto etanólico extracto hidroalcohólico Haemonchus contortus Marroquín-Tun, María de los Ángeles Higuera-Piedrahita, Rosa Isabel López-Arellano, María Eugenia López-Arellano, Raquel de la Cruz-Cruz, Héctor Alejandro Silva-Mendoza, Rocío Cuéllar-Ordaz, Jorge Alfredo In vitro effect of the hydroalcoholic and ethanolic extracts of papaya seeds (Carica papaya) against Haemonchus contortus |
| title | In vitro effect of the hydroalcoholic and ethanolic extracts of papaya seeds (Carica papaya) against Haemonchus contortus |
| title_alt | Efecto in vitro de los extractos hidroalcohólico y etanólico de semilla de papaya (Carica papaya) en Haemonchus contortus |
| title_full | In vitro effect of the hydroalcoholic and ethanolic extracts of papaya seeds (Carica papaya) against Haemonchus contortus |
| title_fullStr | In vitro effect of the hydroalcoholic and ethanolic extracts of papaya seeds (Carica papaya) against Haemonchus contortus |
| title_full_unstemmed | In vitro effect of the hydroalcoholic and ethanolic extracts of papaya seeds (Carica papaya) against Haemonchus contortus |
| title_short | In vitro effect of the hydroalcoholic and ethanolic extracts of papaya seeds (Carica papaya) against Haemonchus contortus |
| title_sort | in vitro effect of the hydroalcoholic and ethanolic extracts of papaya seeds carica papaya against haemonchus contortus |
| topic | Carica papaya ethanolic extract hydro alcoholic extract Haemonchus contortus seeds Carica papaya extracto etanólico extracto hidroalcohólico Haemonchus contortus |
| topic_facet | Carica papaya ethanolic extract hydro alcoholic extract Haemonchus contortus seeds Carica papaya extracto etanólico extracto hidroalcohólico Haemonchus contortus |
| url | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7756 |
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