Quantitative PCR for detection of Citrus tristeza virus in Colombia

Real-time quantitative PCR (qRT-PCR) was applied using SYBR Green for the specific detection of Citrus tristeza virus (CTV) in Colombian. Genomic RNA (gRNA) was amplified using primers designed from conserved sequences in the open reading frames (ORF’s) 1b and 2. We obtained a 186 bp product with ne...

Полное описание

Библиографические подробности
Главные авторы: Solano-Luna, Luis Miguel, Chavarro-Mesa, Edisson, Ángel-Diaz, Jorge Evelio
Формат: Online
Язык:spa
Опубликовано: Universidad Pedagógica y Tecnológica de Colombia 2018
Предметы:
Online-ссылка:https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7789

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