Quantitative PCR for detection of Citrus tristeza virus in Colombia
Real-time quantitative PCR (qRT-PCR) was applied using SYBR Green for the specific detection of Citrus tristeza virus (CTV) in Colombian. Genomic RNA (gRNA) was amplified using primers designed from conserved sequences in the open reading frames (ORF’s) 1b and 2. We obtained a 186 bp product with ne...
| Main Authors: | , , |
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| Format: | Online |
| Language: | spa |
| Published: |
Universidad Pedagógica y Tecnológica de Colombia
2018
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| Subjects: | |
| Online Access: | https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7789 |
