Quantitative PCR for detection of Citrus tristeza virus in Colombia

Quantitative PCR for detection of Citrus tristeza virus in Colombia

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Real-time quantitative PCR (qRT-PCR) was applied using SYBR Green for the specific detection of Citrus tristeza virus (CTV) in Colombian. Genomic RNA (gRNA) was amplified using primers designed from conserved sequences in the open reading frames (ORF’s) 1b and 2. We obtained a 186 bp product with ne...

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Bibliographische Detailangaben
Hauptverfasser: Solano-Luna, Luis Miguel, Chavarro-Mesa, Edisson, Ángel-Diaz, Jorge Evelio
Format: Online
Sprache:spa
Veröffentlicht: Universidad Pedagógica y Tecnológica de Colombia 2018
Schlagworte:
absolute quantification
citrus diseases
Closterovirus
detection of plant pathogens
real-Time PCR SYBR Green
cuantificación absoluta
detección de patógenos vegetales
enfermedades de los cítricos
PCR en tiempo real SYBR Green
Online Zugang:https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7789

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