Quantitative PCR for detection of Citrus tristeza virus in Colombia

Quantitative PCR for detection of Citrus tristeza virus in Colombia

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Real-time quantitative PCR (qRT-PCR) was applied using SYBR Green for the specific detection of Citrus tristeza virus (CTV) in Colombian. Genomic RNA (gRNA) was amplified using primers designed from conserved sequences in the open reading frames (ORF’s) 1b and 2. We obtained a 186 bp product with ne...

書目詳細資料
Main Authors:	Solano-Luna, Luis Miguel, Chavarro-Mesa, Edisson, Ángel-Diaz, Jorge Evelio
格式:	Online
語言:	spa
出版:	Universidad Pedagógica y Tecnológica de Colombia 2018
主題:	absolute quantification citrus diseases Closterovirus detection of plant pathogens real-Time PCR SYBR Green cuantificación absoluta detección de patógenos vegetales enfermedades de los cítricos PCR en tiempo real SYBR Green
在線閱讀:	https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7789

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