Quantitative PCR for detection of Citrus tristeza virus in Colombia

Real-time quantitative PCR (qRT-PCR) was applied using SYBR Green for the specific detection of Citrus tristeza virus (CTV) in Colombian. Genomic RNA (gRNA) was amplified using primers designed from conserved sequences in the open reading frames (ORF’s) 1b and 2. We obtained a 186 bp product with ne...

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Main Authors: Solano-Luna, Luis Miguel, Chavarro-Mesa, Edisson, Ángel-Diaz, Jorge Evelio
格式: Online
语言:spa
出版: Universidad Pedagógica y Tecnológica de Colombia 2018
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在线阅读:https://revistas.uptc.edu.co/index.php/ciencia_agricultura/article/view/7789