Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro
Rhizoctonia solani is a fungus that affects the production of tubers and vigor of potato plants, Solanum tuberosum L. The environmental consequences generated by its control have driven the search for less contaminating alternatives, and essential oils have great potential. The wild shrub Lippia ori...
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| Format: | Online |
| Language: | spa |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2018
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7801 |
| _version_ | 1801706281201827840 |
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| author | Álvarez-Sánchez, David Hurtado-Benavides, Andrés Chaves-Morillo, Diana Andrade-Díaz, Danita |
| author_facet | Álvarez-Sánchez, David Hurtado-Benavides, Andrés Chaves-Morillo, Diana Andrade-Díaz, Danita |
| author_sort | Álvarez-Sánchez, David |
| collection | OJS |
| description | Rhizoctonia solani is a fungus that affects the production of tubers and vigor of potato plants, Solanum tuberosum L. The environmental consequences generated by its control have driven the search for less contaminating alternatives, and essential oils have great potential. The wild shrub Lippia origanoides H.B.K. (Verbenaceae) has been studied because of its attractive fungicidal properties in its essential extracts; therefore, the objective of this research was to evaluate the biocidal activity of L. origanoides essential oil on R. solani, in vitro. The oil extraction was carried out with steam dragging, the chemical composition was determined with Gas Chromatography-Mass Spectrometry (GC-MS), and, finally, the biocidal activity of the oil was evaluated with the dilution technique in the culture medium, which was complemented with the bioautography technique, which identified thymol as the major compound of the oil (60.7%), responsible for the fungus inhibition. The concentration 110 μL mL-1 limited the growth of R. solani by affecting the structures, thereby demonstrating the biocidal effect of this essential oil and its potential antifungal agent. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-7801 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | spa |
| publishDate | 2018 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-78012020-08-03T23:56:24Z Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro Actividad biocida del aceite esencial de Lippia origanoides H.B.K. (Verbenaceae) sobre Rhizoctonia solani: in vitro Álvarez-Sánchez, David Hurtado-Benavides, Andrés Chaves-Morillo, Diana Andrade-Díaz, Danita Oregano Thymol Solanum tuberosum Thin-layer chromatography Essential oils crop protection Orégano Timol Solanum tuberosum Cromatografía en capa fina Aceites esenciales protección de cultivos Rhizoctonia solani is a fungus that affects the production of tubers and vigor of potato plants, Solanum tuberosum L. The environmental consequences generated by its control have driven the search for less contaminating alternatives, and essential oils have great potential. The wild shrub Lippia origanoides H.B.K. (Verbenaceae) has been studied because of its attractive fungicidal properties in its essential extracts; therefore, the objective of this research was to evaluate the biocidal activity of L. origanoides essential oil on R. solani, in vitro. The oil extraction was carried out with steam dragging, the chemical composition was determined with Gas Chromatography-Mass Spectrometry (GC-MS), and, finally, the biocidal activity of the oil was evaluated with the dilution technique in the culture medium, which was complemented with the bioautography technique, which identified thymol as the major compound of the oil (60.7%), responsible for the fungus inhibition. The concentration 110 μL mL-1 limited the growth of R. solani by affecting the structures, thereby demonstrating the biocidal effect of this essential oil and its potential antifungal agent. Rhizoctonia solani es un hongo que afecta la producción de tubérculos y vigor de las plantas de papa Solanum tuberosum L. Las consecuencias ambientales que genera su control han impulsado la búsqueda de alternativas menos contaminantes, en donde los aceites esenciales tienen gran potencial. El arbusto silvestre Lippia origanoides H.B.K. (Verbenaceae) ha sido estudiado por sus propiedades fungicidas en su extracto esencial, por ello, el objetivo de esta investigación fue evaluar la actividad biocida del aceite esencial de L. origanoides sobre R. solani en condiciones in vitro. La extracción del aceite se realizó mediante arrastre con vapor. La composición química se determinó por cromatografía de gases-espectrometría de masas (GC-MS) y la actividad biocida del aceite se evaluó mediante la técnica de dilución en medio de cultivo, complementando los resultados con la técnica de bioautografía, la cual permitió identificar al timol como el compuesto mayoritario del aceite (60,7%) y responsable de la inhibición del hongo. La concentración 110 µL mL-1 limitó el crecimiento de R. solani al afectar sus estructuras, con ello se demostró el efecto biocida del aceite esencial y el potencial agente antifúngico natural. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto Texte Testo Text application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7801 10.17584/rcch.2018v12i3.7801 Revista Colombiana de Ciencias Hortícolas; Vol. 12 No. 3 (2018); 668-676 Revista Colombiana de Ciencias Hortícolas; Vol. 12 Núm. 3 (2018); 668-676 Revista Colombiana de Ciencias Hortícolas; Vol. 12 No 3 (2018); 668-676 Revista Colombiana de Ciencias Hortícolas; V. 12 N. 3 (2018); 668-676 Revista Colombiana de Ciencias Hortícolas; v. 12 n. 3 (2018); 668-676 2422-3719 2011-2173 spa https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7801/8556 Colombia; Nariño Colombia; Nariño Colombia; Nariño Colombia; Nariño Colombia; Nariño Copyright (c) 2018 Revista Colombiana de Ciencias Hortícolas |
| spellingShingle | Oregano Thymol Solanum tuberosum Thin-layer chromatography Essential oils crop protection Orégano Timol Solanum tuberosum Cromatografía en capa fina Aceites esenciales protección de cultivos Álvarez-Sánchez, David Hurtado-Benavides, Andrés Chaves-Morillo, Diana Andrade-Díaz, Danita Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro |
| title | Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro |
| title_alt | Actividad biocida del aceite esencial de Lippia origanoides H.B.K. (Verbenaceae) sobre Rhizoctonia solani: in vitro |
| title_full | Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro |
| title_fullStr | Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro |
| title_full_unstemmed | Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro |
| title_short | Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro |
| title_sort | biocidal activity of lippia origanoides h b k verbenaceae essential oil on rhizoctonia solani in vitro |
| topic | Oregano Thymol Solanum tuberosum Thin-layer chromatography Essential oils crop protection Orégano Timol Solanum tuberosum Cromatografía en capa fina Aceites esenciales protección de cultivos |
| topic_facet | Oregano Thymol Solanum tuberosum Thin-layer chromatography Essential oils crop protection Orégano Timol Solanum tuberosum Cromatografía en capa fina Aceites esenciales protección de cultivos |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7801 |
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