Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro

Rhizoctonia solani is a fungus that affects the production of tubers and vigor of potato plants, Solanum tuberosum L. The environmental consequences generated by its control have driven the search for less contaminating alternatives, and essential oils have great potential. The wild shrub Lippia ori...

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Main Authors:	Álvarez-Sánchez, David, Hurtado-Benavides, Andrés, Chaves-Morillo, Diana, Andrade-Díaz, Danita
Format:	Online
Language:	spa
Published:	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018
Subjects:	Oregano Thymol Solanum tuberosum Thin-layer chromatography Essential oils crop protection Orégano Timol Cromatografía en capa fina Aceites esenciales protección de cultivos
Online Access:	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7801
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author	Álvarez-Sánchez, David Hurtado-Benavides, Andrés Chaves-Morillo, Diana Andrade-Díaz, Danita
author_facet	Álvarez-Sánchez, David Hurtado-Benavides, Andrés Chaves-Morillo, Diana Andrade-Díaz, Danita
author_sort	Álvarez-Sánchez, David
collection	OJS
description	Rhizoctonia solani is a fungus that affects the production of tubers and vigor of potato plants, Solanum tuberosum L. The environmental consequences generated by its control have driven the search for less contaminating alternatives, and essential oils have great potential. The wild shrub Lippia origanoides H.B.K. (Verbenaceae) has been studied because of its attractive fungicidal properties in its essential extracts; therefore, the objective of this research was to evaluate the biocidal activity of L. origanoides essential oil on R. solani, in vitro. The oil extraction was carried out with steam dragging, the chemical composition was determined with Gas Chromatography-Mass Spectrometry (GC-MS), and, finally, the biocidal activity of the oil was evaluated with the dilution technique in the culture medium, which was complemented with the bioautography technique, which identified thymol as the major compound of the oil (60.7%), responsible for the fungus inhibition. The concentration 110 μL mL-1 limited the growth of R. solani by affecting the structures, thereby demonstrating the biocidal effect of this essential oil and its potential antifungal agent.
format	Online
id	oai:oai.revistas.uptc.edu.co:article-7801
institution	Revista Colombiana de Ciencias Hortícolas
language	spa
publishDate	2018
publisher	Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
record_format	ojs
spelling	oai:oai.revistas.uptc.edu.co:article-78012020-08-03T23:56:24Z Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro Actividad biocida del aceite esencial de Lippia origanoides H.B.K. (Verbenaceae) sobre Rhizoctonia solani: in vitro Álvarez-Sánchez, David Hurtado-Benavides, Andrés Chaves-Morillo, Diana Andrade-Díaz, Danita Oregano Thymol Solanum tuberosum Thin-layer chromatography Essential oils crop protection Orégano Timol Solanum tuberosum Cromatografía en capa fina Aceites esenciales protección de cultivos Rhizoctonia solani is a fungus that affects the production of tubers and vigor of potato plants, Solanum tuberosum L. The environmental consequences generated by its control have driven the search for less contaminating alternatives, and essential oils have great potential. The wild shrub Lippia origanoides H.B.K. (Verbenaceae) has been studied because of its attractive fungicidal properties in its essential extracts; therefore, the objective of this research was to evaluate the biocidal activity of L. origanoides essential oil on R. solani, in vitro. The oil extraction was carried out with steam dragging, the chemical composition was determined with Gas Chromatography-Mass Spectrometry (GC-MS), and, finally, the biocidal activity of the oil was evaluated with the dilution technique in the culture medium, which was complemented with the bioautography technique, which identified thymol as the major compound of the oil (60.7%), responsible for the fungus inhibition. The concentration 110 μL mL-1 limited the growth of R. solani by affecting the structures, thereby demonstrating the biocidal effect of this essential oil and its potential antifungal agent. Rhizoctonia solani es un hongo que afecta la producción de tubérculos y vigor de las plantas de papa Solanum tuberosum L. Las consecuencias ambientales que genera su control han impulsado la búsqueda de alternativas menos contaminantes, en donde los aceites esenciales tienen gran potencial. El arbusto silvestre Lippia origanoides H.B.K. (Verbenaceae) ha sido estudiado por sus propiedades fungicidas en su extracto esencial, por ello, el objetivo de esta investigación fue evaluar la actividad biocida del aceite esencial de L. origanoides sobre R. solani en condiciones in vitro. La extracción del aceite se realizó mediante arrastre con vapor. La composición química se determinó por cromatografía de gases-espectrometría de masas (GC-MS) y la actividad biocida del aceite se evaluó mediante la técnica de dilución en medio de cultivo, complementando los resultados con la técnica de bioautografía, la cual permitió identificar al timol como el compuesto mayoritario del aceite (60,7%) y responsable de la inhibición del hongo. La concentración 110 µL mL-1 limitó el crecimiento de R. solani al afectar sus estructuras, con ello se demostró el efecto biocida del aceite esencial y el potencial agente antifúngico natural. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2018-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto Texte Testo Text application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7801 10.17584/rcch.2018v12i3.7801 Revista Colombiana de Ciencias Hortícolas; Vol. 12 No. 3 (2018); 668-676 Revista Colombiana de Ciencias Hortícolas; Vol. 12 Núm. 3 (2018); 668-676 Revista Colombiana de Ciencias Hortícolas; Vol. 12 No 3 (2018); 668-676 Revista Colombiana de Ciencias Hortícolas; V. 12 N. 3 (2018); 668-676 Revista Colombiana de Ciencias Hortícolas; v. 12 n. 3 (2018); 668-676 2422-3719 2011-2173 spa https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7801/8556 Colombia; Nariño Colombia; Nariño Colombia; Nariño Colombia; Nariño Colombia; Nariño Copyright (c) 2018 Revista Colombiana de Ciencias Hortícolas
spellingShingle	Oregano Thymol Solanum tuberosum Thin-layer chromatography Essential oils crop protection Orégano Timol Solanum tuberosum Cromatografía en capa fina Aceites esenciales protección de cultivos Álvarez-Sánchez, David Hurtado-Benavides, Andrés Chaves-Morillo, Diana Andrade-Díaz, Danita Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro
title	Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro
title_alt	Actividad biocida del aceite esencial de Lippia origanoides H.B.K. (Verbenaceae) sobre Rhizoctonia solani: in vitro
title_full	Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro
title_fullStr	Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro
title_full_unstemmed	Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro
title_short	Biocidal activity of Lippia origanoides H.B.K. (Verbenaceae) essential oil on Rhizoctonia solani: in vitro
title_sort	biocidal activity of lippia origanoides h b k verbenaceae essential oil on rhizoctonia solani in vitro
topic	Oregano Thymol Solanum tuberosum Thin-layer chromatography Essential oils crop protection Orégano Timol Solanum tuberosum Cromatografía en capa fina Aceites esenciales protección de cultivos
topic_facet	Oregano Thymol Solanum tuberosum Thin-layer chromatography Essential oils crop protection Orégano Timol Solanum tuberosum Cromatografía en capa fina Aceites esenciales protección de cultivos
url	https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/7801
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