Diazotrophic bacteria in the growth of micropropagated ornamental pineapple
Ornamental pineapple is a hardy plant with significant landscaping value. Tissue culture of plants is viable for producing plants with a high phytosanitary quality. However, one of the difficulties with this cultivar is the acclimatization process, which is slow and can cause losses. The objective o...
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| Format: | Online |
| Language: | eng |
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Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC
2019
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| Online Access: | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/8034 |
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| author | Silva, Adriano Bortolotti Florentino, Ligiane Aparecida Pereira, Dalvana de Sousa Landgraf, Paulo Roberto Correa Alves, Ana Carolina Rodrigues Santos-Filho, Plinio Rodrigues dos |
| author_facet | Silva, Adriano Bortolotti Florentino, Ligiane Aparecida Pereira, Dalvana de Sousa Landgraf, Paulo Roberto Correa Alves, Ana Carolina Rodrigues Santos-Filho, Plinio Rodrigues dos |
| author_sort | Silva, Adriano Bortolotti |
| collection | OJS |
| description | Ornamental pineapple is a hardy plant with significant landscaping value. Tissue culture of plants is viable for producing plants with a high phytosanitary quality. However, one of the difficulties with this cultivar is the acclimatization process, which is slow and can cause losses. The objective of the present study was to verify the potential of inoculation with diazotrophic bacteria for in vitro and ex vivo growth of ornamental pineapple. A group of diazotrophic bacterial strains selected at the Universidade José do Rosário Vellano (UNIFENAS) was prioritized in this study, and the treatments included bacterial strains UNIFENAS (100-13, 100-60, 100-68, 100-153, 100-167 and 100-198). These strains were evaluated in terms of their capacity to produce indole 3-acetic acid. Subsequently, plants were cultivated in a medium composed of MS medium salts (1/4), adding 1 mL of the bacterial strain. In the control treatment, the plants were maintained in 2 mL of MS medium. 7 days after inoculation, the plants were transplanted into the MS, where they were maintained for 30 days. After in vitro cultivation, the plants were transferred to pots containing commercial Plantmax® substrate and maintained under these conditions for 60 days. The diazotrophic bacteria were able to synthesize auxins, and their inoculation promoted greater growth in vitro and ex vitro in the plants. In the acclimatization phase, the plants inoculated with UNIFENAS strains (100-60, 100-68 and 100-153) promoted a higher shoot growth, chlorophyll content and nitrate reductase enzyme activity. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-8034 |
| institution | Revista Colombiana de Ciencias Hortícolas |
| language | eng |
| publishDate | 2019 |
| publisher | Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-80342020-08-04T02:23:43Z Diazotrophic bacteria in the growth of micropropagated ornamental pineapple Bacterias diazotróficas en el crecimiento de la piña ornamental micropropagada Silva, Adriano Bortolotti Florentino, Ligiane Aparecida Pereira, Dalvana de Sousa Landgraf, Paulo Roberto Correa Alves, Ana Carolina Rodrigues Santos-Filho, Plinio Rodrigues dos Ananas In vitro Bacterial strains Acclimatization Pigments Nitrate reductase activity Propagation in vitro Ananas In vitro Cepas bacterianas Aclimatación Pigmentos Actividad nitrato reductasa Propagación in vitro Ornamental pineapple is a hardy plant with significant landscaping value. Tissue culture of plants is viable for producing plants with a high phytosanitary quality. However, one of the difficulties with this cultivar is the acclimatization process, which is slow and can cause losses. The objective of the present study was to verify the potential of inoculation with diazotrophic bacteria for in vitro and ex vivo growth of ornamental pineapple. A group of diazotrophic bacterial strains selected at the Universidade José do Rosário Vellano (UNIFENAS) was prioritized in this study, and the treatments included bacterial strains UNIFENAS (100-13, 100-60, 100-68, 100-153, 100-167 and 100-198). These strains were evaluated in terms of their capacity to produce indole 3-acetic acid. Subsequently, plants were cultivated in a medium composed of MS medium salts (1/4), adding 1 mL of the bacterial strain. In the control treatment, the plants were maintained in 2 mL of MS medium. 7 days after inoculation, the plants were transplanted into the MS, where they were maintained for 30 days. After in vitro cultivation, the plants were transferred to pots containing commercial Plantmax® substrate and maintained under these conditions for 60 days. The diazotrophic bacteria were able to synthesize auxins, and their inoculation promoted greater growth in vitro and ex vitro in the plants. In the acclimatization phase, the plants inoculated with UNIFENAS strains (100-60, 100-68 and 100-153) promoted a higher shoot growth, chlorophyll content and nitrate reductase enzyme activity. La piña ornamental es una planta rústica y de alto valor paisajístico. El cultivo de tejidos de la especie se muestra viable, produciendo plantas con alta calidad fitosanitaria. Una de las dificultades de ese cultivo es el proceso de aclimatación, que ocurre lentamente y puede causar pérdidas. El presente estudio tuvo como objetivo verificar el potencial de inoculación de las bacterias diazotróficas en el crecimiento in vitro y ex vitro de la planta de la piña ornamental. Un grupo de cepas de bacterias diazotróficas seleccionadas en la Universidade José do Rosário Vellano (UNIFENAS) fueron usadas en el estudio y las cepas bacterianas UNIFENAS 100-13, 100-60, 100-68, 100-153, 100-167 y 100-198, constituyeron los tratamientos. Se evaluaron las cepas con relación a la capacidad de producir ácido indol-3-acético. Posteriormente, las plantas fueron cultivadas en medio MS (1/4) y 1 mL de la cepa bacteriana. En el tratamiento control se mantuvieron las plantas con 2 mL de medio MS. Después de 7 días de la inoculación, las plantas fueron trasplantadas a MS, donde permanecieron por un período de 30 días. Después del cultivo in vitro, las plantas fueron transferidas a materas con el substrato comercial Plantmax®, donde se mantuvieron por 60 días en estas condiciones. Las bacterias son capaces de sintetizar auxinas y su inoculación promueve mayor crecimiento de las plantas in vitro y ex vitro. En la fase de aclimatación, las plantas inoculadas con cepas UNIFENAS 100-60, 100-68 y 100-153, promovieron un mayor crecimiento de brotes, un mayor contenido de clorofila y una actividad de la enzima nitrato reductasa. Sociedad Colombiana de Ciencias Hortícolas-SCCH and Universidad Pedagógica y Tecnológica de Colombia-UPTC 2019-05-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Text Texto Texte Testo Texto application/pdf https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/8034 10.17584/rcch.2019v13i2.8034 Revista Colombiana de Ciencias Hortícolas; Vol. 13 No. 2 (2019); 269-278 Revista Colombiana de Ciencias Hortícolas; Vol. 13 Núm. 2 (2019); 269-278 Revista Colombiana de Ciencias Hortícolas; Vol. 13 No 2 (2019); 269-278 Revista Colombiana de Ciencias Hortícolas; V. 13 N. 2 (2019); 269-278 Revista Colombiana de Ciencias Hortícolas; v. 13 n. 2 (2019); 269-278 2422-3719 2011-2173 eng https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/8034/8771 Brazil; MG; Alfenas Brazil; MG; Alfenas Brazil; MG; Alfenas Brazil; MG; Alfenas Brazil; MG; Alfenas Copyright (c) 2019 Revista Colombiana de Ciencias Hortícolas |
| spellingShingle | Ananas In vitro Bacterial strains Acclimatization Pigments Nitrate reductase activity Propagation in vitro Ananas In vitro Cepas bacterianas Aclimatación Pigmentos Actividad nitrato reductasa Propagación in vitro Silva, Adriano Bortolotti Florentino, Ligiane Aparecida Pereira, Dalvana de Sousa Landgraf, Paulo Roberto Correa Alves, Ana Carolina Rodrigues Santos-Filho, Plinio Rodrigues dos Diazotrophic bacteria in the growth of micropropagated ornamental pineapple |
| title | Diazotrophic bacteria in the growth of micropropagated ornamental pineapple |
| title_alt | Bacterias diazotróficas en el crecimiento de la piña ornamental micropropagada |
| title_full | Diazotrophic bacteria in the growth of micropropagated ornamental pineapple |
| title_fullStr | Diazotrophic bacteria in the growth of micropropagated ornamental pineapple |
| title_full_unstemmed | Diazotrophic bacteria in the growth of micropropagated ornamental pineapple |
| title_short | Diazotrophic bacteria in the growth of micropropagated ornamental pineapple |
| title_sort | diazotrophic bacteria in the growth of micropropagated ornamental pineapple |
| topic | Ananas In vitro Bacterial strains Acclimatization Pigments Nitrate reductase activity Propagation in vitro Ananas In vitro Cepas bacterianas Aclimatación Pigmentos Actividad nitrato reductasa Propagación in vitro |
| topic_facet | Ananas In vitro Bacterial strains Acclimatization Pigments Nitrate reductase activity Propagation in vitro Ananas In vitro Cepas bacterianas Aclimatación Pigmentos Actividad nitrato reductasa Propagación in vitro |
| url | https://revistas.uptc.edu.co/index.php/ciencias_horticolas/article/view/8034 |
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