Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen
The present investigation focuses on the evaluation of the enzymatic hydrolysis method assisted by ultrasound in obtaining hydrolyzed collagen from the use of the residue (wet white) from leather tanned with glutaraldehyde, in order to minimize the generation of environmental impacts that have occur...
| Main Authors: | , , , , , |
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| Format: | Online |
| Language: | spa |
| Published: |
Universidad Pedagógica y Tecnológica de Colombia
2019
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| Subjects: | |
| Online Access: | https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654 |
| _version_ | 1801706096831758336 |
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| author | Almonacid-Jiménez, Leidy Yaneth Vallejo-Rodríguez, Juan Sebastián Agudelo-Valencia, Rafael Nikolay Hernández-Fernández, Javier Adolfo Ortiz-Medina, Óscar Leonardo Ovalle-González, Diana Paola |
| author_facet | Almonacid-Jiménez, Leidy Yaneth Vallejo-Rodríguez, Juan Sebastián Agudelo-Valencia, Rafael Nikolay Hernández-Fernández, Javier Adolfo Ortiz-Medina, Óscar Leonardo Ovalle-González, Diana Paola |
| author_sort | Almonacid-Jiménez, Leidy Yaneth |
| collection | OJS |
| description | The present investigation focuses on the evaluation of the enzymatic hydrolysis method assisted by ultrasound in obtaining hydrolyzed collagen from the use of the residue (wet white) from leather tanned with glutaraldehyde, in order to minimize the generation of environmental impacts that have occurred for such material. The effect of pH of the solution, enzyme dose, intensity of applied ultrasound, and temperature of the mixture were evaluated, keeping constant the initial mass of wet white, volume of water and reaction time. The experimental results indicate that for pH 9 doses of enzyme Tan G Plus 0.00012 kg, temperature of 313.15 K and intensity of ultrasound of 40 % it is possible to degrade the material and denature the protein, obtaining a hydrolyzed collagen weight concentration of 3.013 kg / m3, for each kilogram of material used, which represents a 3.0% mass recovery of wet white. It is observed that the most representative variables for the analyzed process are the pH and the temperature, giving a higher performance to the process allowing get a higher amount of hydrolyzed protein. |
| format | Online |
| id | oai:oai.revistas.uptc.edu.co:article-9654 |
| institution | Revista Facultad de Ingeniería |
| language | spa |
| publishDate | 2019 |
| publisher | Universidad Pedagógica y Tecnológica de Colombia |
| record_format | ojs |
| spelling | oai:oai.revistas.uptc.edu.co:article-96542021-07-13T02:25:37Z Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen Evaluación de la hidrólisis enzimática de wet white asistida con ultrasonido para obtener colágeno hidrolizado Almonacid-Jiménez, Leidy Yaneth Vallejo-Rodríguez, Juan Sebastián Agudelo-Valencia, Rafael Nikolay Hernández-Fernández, Javier Adolfo Ortiz-Medina, Óscar Leonardo Ovalle-González, Diana Paola collagen environmental impacts enzymatic hydrolysis ultrasound wet white colágeno hidrólisis enzimática impactos ambientales ultrasonido wet white The present investigation focuses on the evaluation of the enzymatic hydrolysis method assisted by ultrasound in obtaining hydrolyzed collagen from the use of the residue (wet white) from leather tanned with glutaraldehyde, in order to minimize the generation of environmental impacts that have occurred for such material. The effect of pH of the solution, enzyme dose, intensity of applied ultrasound, and temperature of the mixture were evaluated, keeping constant the initial mass of wet white, volume of water and reaction time. The experimental results indicate that for pH 9 doses of enzyme Tan G Plus 0.00012 kg, temperature of 313.15 K and intensity of ultrasound of 40 % it is possible to degrade the material and denature the protein, obtaining a hydrolyzed collagen weight concentration of 3.013 kg / m3, for each kilogram of material used, which represents a 3.0% mass recovery of wet white. It is observed that the most representative variables for the analyzed process are the pH and the temperature, giving a higher performance to the process allowing get a higher amount of hydrolyzed protein. La presente investigación se centra en la evaluación del método de hidrólisis enzimática asistida por ultrasonido en la obtención de colágeno hidrolizado a partir del aprovechamiento del residuo (wet white) proveniente del cuero curtido con glutaraldehído, con la finalidad de minimizar la generación de impactos ambientales por dicho material. Se evaluó el efecto del pH de la solución, dosis de enzima, intensidad de ultrasonido aplicado y temperatura de la mezcla, manteniendo constantes la masa inicial de wet white, el volumen de agua y el tiempo de reacción. Los resultados experimentales señalan que para pH 9 dosis de enzima Tan G Plus 0.00012 kg, temperatura de 313.15 K e intensidad de ultrasonido del 40 % se logra degradar el material y desnaturalizar la proteína, obteniendo una concentración en peso de colágeno hidrolizado de 3.013 kg/m3, por cada kilogramo de material empleado, el cual representa una recuperación del 3.0 % en masa de wet white. Se observa que las variables más representativas para el proceso analizado son el pH y la temperatura, que dan un mayor rendimiento al proceso y permiten obtener mayor cantidad de proteína hidrolizada. Universidad Pedagógica y Tecnológica de Colombia 2019-07-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion research investigación application/pdf application/xml https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654 10.19053/01211129.v28.n52.2019.9654 Revista Facultad de Ingeniería; Vol. 28 No. 52 (2019); 59-75 Revista Facultad de Ingeniería; Vol. 28 Núm. 52 (2019); 59-75 2357-5328 0121-1129 spa https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654/8016 https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654/8249 N.A. N.A. |
| spellingShingle | collagen environmental impacts enzymatic hydrolysis ultrasound wet white colágeno hidrólisis enzimática impactos ambientales ultrasonido wet white Almonacid-Jiménez, Leidy Yaneth Vallejo-Rodríguez, Juan Sebastián Agudelo-Valencia, Rafael Nikolay Hernández-Fernández, Javier Adolfo Ortiz-Medina, Óscar Leonardo Ovalle-González, Diana Paola Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen |
| title | Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen |
| title_alt | Evaluación de la hidrólisis enzimática de wet white asistida con ultrasonido para obtener colágeno hidrolizado |
| title_full | Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen |
| title_fullStr | Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen |
| title_full_unstemmed | Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen |
| title_short | Evaluation of the Enzymatic Hydrolysis of Wet White Assisted with Ultrasound to Obtain Hydrolyzed Collagen |
| title_sort | evaluation of the enzymatic hydrolysis of wet white assisted with ultrasound to obtain hydrolyzed collagen |
| topic | collagen environmental impacts enzymatic hydrolysis ultrasound wet white colágeno hidrólisis enzimática impactos ambientales ultrasonido wet white |
| topic_facet | collagen environmental impacts enzymatic hydrolysis ultrasound wet white colágeno hidrólisis enzimática impactos ambientales ultrasonido wet white |
| url | https://revistas.uptc.edu.co/index.php/ingenieria/article/view/9654 |
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